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Literature summary for 1.3.1.75 extracted from

  • Yamamoto, H.; Mizoguchi, T.; Tsukatani, Y.; Tamiaki, H.; Kurisu, G.; Fujita, Y.
    Chlorophyllide a oxidoreductase preferentially catalyzes 8-vinyl reduction over B-ring reduction of 8-vinyl chlorophyllide a in the late steps of bacteriochlorophyll biosynthesis (2020), ChemBioChem, 21, 1760-1766 .
    View publication on PubMed
Search for more literature for 1.3.1.75

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
 8-vinyl chlorophyllide a pH and temperature not specified in the publication Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,8-divinyl chlorophyllide a + NADPH + H+ Chlorobaculum tepidum preferred substrate chlorophyllide a + NADP+
-
 ?
3,8-divinyl chlorophyllide a + NADPH + H+ Chlorobaculum tepidum ATCC 49652 preferred substrate chlorophyllide a + NADP+
-
 ?
3,8-divinyl chlorophyllide a + NADPH + H+ Chlorobaculum tepidum TLS0 preferred substrate chlorophyllide a + NADP+
-
 ?
3,8-divinyl chlorophyllide a + NADPH + H+ Chlorobaculum tepidum DSM 12025 preferred substrate chlorophyllide a + NADP+
-
 ?
3,8-divinyl chlorophyllide a + NADPH + H+ Chlorobaculum tepidum NBRC 103806 preferred substrate chlorophyllide a + NADP+
-
 ?
3,8-divinyl protochlorophyllide a + NADPH + H+ Chlorobaculum tepidum
-
 protochlorophyllide a + NADP+
-
 ?
3,8-divinyl protochlorophyllide a + NADPH + H+ Chlorobaculum tepidum ATCC 49652
-
 protochlorophyllide a + NADP+
-
 ?
3,8-divinyl protochlorophyllide a + NADPH + H+ Chlorobaculum tepidum TLS0
-
 protochlorophyllide a + NADP+
-
 ?
3,8-divinyl protochlorophyllide a + NADPH + H+ Chlorobaculum tepidum DSM 12025
-
 protochlorophyllide a + NADP+
-
 ?
3,8-divinyl protochlorophyllide a + NADPH + H+ Chlorobaculum tepidum NBRC 103806
-
 protochlorophyllide a + NADP+
-
 ?
8-vinyl chlorophyllide a + NADPH + H+ Rhodobacter capsulatus preferred reaction of BciA chlorophyllide a + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Chlorobaculum tepidum Q8KDI7 Chlorobium tepidum
-
Chlorobaculum tepidum ATCC 49652 Q8KDI7 Chlorobium tepidum
-
Chlorobaculum tepidum DSM 12025 Q8KDI7 Chlorobium tepidum
-
Chlorobaculum tepidum NBRC 103806 Q8KDI7 Chlorobium tepidum
-
Chlorobaculum tepidum TLS0 Q8KDI7 Chlorobium tepidum
-
Rhodobacter capsulatus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1
-
 recombinant BciA, pH and temperature not specified in the publication Chlorobaculum tepidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,8-divinyl chlorophyllide a + NADPH + H+
-
 Chlorobaculum tepidum chlorophyllide a + NADP+
-
 ?
3,8-divinyl chlorophyllide a + NADPH + H+ preferred substrate Chlorobaculum tepidum chlorophyllide a + NADP+
-
 ?
3,8-divinyl chlorophyllide a + NADPH + H+
-
 Chlorobaculum tepidum ATCC 49652 chlorophyllide a + NADP+
-
 ?
3,8-divinyl chlorophyllide a + NADPH + H+ preferred substrate Chlorobaculum tepidum ATCC 49652 chlorophyllide a + NADP+
-
 ?
3,8-divinyl chlorophyllide a + NADPH + H+
-
 Chlorobaculum tepidum TLS0 chlorophyllide a + NADP+
-
 ?
3,8-divinyl chlorophyllide a + NADPH + H+ preferred substrate Chlorobaculum tepidum TLS0 chlorophyllide a + NADP+
-
 ?
3,8-divinyl chlorophyllide a + NADPH + H+
-
 Chlorobaculum tepidum DSM 12025 chlorophyllide a + NADP+
-
 ?
3,8-divinyl chlorophyllide a + NADPH + H+ preferred substrate Chlorobaculum tepidum DSM 12025 chlorophyllide a + NADP+
-
 ?
3,8-divinyl chlorophyllide a + NADPH + H+
-
 Chlorobaculum tepidum NBRC 103806 chlorophyllide a + NADP+
-
 ?
3,8-divinyl chlorophyllide a + NADPH + H+ preferred substrate Chlorobaculum tepidum NBRC 103806 chlorophyllide a + NADP+
-
 ?
3,8-divinyl protochlorophyllide a + NADPH + H+
-
 Chlorobaculum tepidum protochlorophyllide a + NADP+
-
 ?
3,8-divinyl protochlorophyllide a + NADPH + H+
-
 Chlorobaculum tepidum ATCC 49652 protochlorophyllide a + NADP+
-
 ?
3,8-divinyl protochlorophyllide a + NADPH + H+
-
 Chlorobaculum tepidum TLS0 protochlorophyllide a + NADP+
-
 ?
3,8-divinyl protochlorophyllide a + NADPH + H+
-
 Chlorobaculum tepidum DSM 12025 protochlorophyllide a + NADP+
-
 ?
3,8-divinyl protochlorophyllide a + NADPH + H+
-
 Chlorobaculum tepidum NBRC 103806 protochlorophyllide a + NADP+
-
 ?
8-vinyl chlorophyllide a + NADPH + H+ preferred reaction of BciA Rhodobacter capsulatus chlorophyllide a + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
8VR
-
 Chlorobaculum tepidum
BciA
-
 Rhodobacter capsulatus
BciA
-
 Chlorobaculum tepidum
C8 vinyl reductase
-
 Rhodobacter capsulatus
C8 vinyl reductase
-
 Chlorobaculum tepidum
cvrA
-
 Chlorobaculum tepidum
divinyl chlorophyllide a 8-vinyl-reductase, chloroplastic UniProt Chlorobaculum tepidum
NADPH-dependent 8VR
-
 Rhodobacter capsulatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.66
-
 8-vinyl chlorophyllide a pH and temperature not specified in the publication Rhodobacter capsulatus
0.66
-
 3,8-divinyl chlorophyllide a recombinant BciA, pH and temperature not specified in the publication Chlorobaculum tepidum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Chlorobaculum tepidum

General Information

General Information Comment Organism
metabolism bacteriochlorophyll a (BChl) is an essential pigment for anoxygenic photosynthesis. In late steps of the BChl biosynthesis, the C8 vinyl group and C7=C8 double bond of 8-vinyl chlorophyllide a (8 V-Chlide) are reduced by a C8 vinyl reductase (8VR), BciA or BciB (EC 1.3.7.13), and a nitrogenase-like enzyme, chlorophyllide a oxidoreductase (COR, EC 1.3.7.15), respectively, to produce 3-vinyl-bacteriochlorphyllide a Chlorobaculum tepidum
physiological function bacteriochlorophyll a (BChl) is an essential pigment for anoxygenic photosynthesis. In late steps of the BChl biosynthesis, the C8 vinyl group and C7=C8 double bond of 8-vinyl chlorophyllide a (8 V-Chlide) are reduced by a C8 vinyl reductase (8VR), BciA or BciB (EC 1.3.7.13), and a nitrogenase-like enzyme, chlorophyllide a oxidoreductase (COR), respectively, to produce 3-vinyl-bacteriochlorphyllide a. BciB is a FAD-containing FeS protein that uses reduced ferredoxin as the reductant for the 8-vinyl reduction of 8 V-Chlide or 8 V-PChlide, while BciA utilizes NADPH. BciA is an NADPH-dependent 8VR with a preference for the 8-vinyl chlorophyllide a (8 V-Chlide) substrate compared with 8-vinyl PChlide (8 V-PChlide) Chlorobaculum tepidum