BRENDA - Reference to 1.3.1.75; Id = 745981

additional information

construction of mutant tepdA lacking enzymes BciA and BchU, that catalyze reduction of the C8-vinyl group and methylation at the C20 position of bacteriochlorophyll (BChl) c, respectively, in the green sulfur bacterium Chlorobaculum tepidum, the mutant accumula C8-vinyl-bacteriochlorophyllide d derivative, determined by NMR to be (31R)-8-vinyl-12-ethyl-(R[V,E])BChl d. The bciA mutant possesses mainly (31R)-8-vinyl-12-ethyl-(R[V,E])BChl c as in chlorosomal self-aggregates in addition to minor (31R)-8-vinyl-12-methyl-(R[V,M]) and (31S)-8-vinyl-12-ethyl-(S[V,E])BChls c. Reconstitution of self-aggregates of C8V-BChl species in vitro. Comparison of chlorophyllide a derivatives in tepdA strains lacking bciA and/or bciU, overview

Chlorobaculum tepidum

3,8-divinyl bacteriochlorophyllide a + NADPH + H+

Chlorobaculum tepidum

-

bacteriochlorophyllide a + NADP+

-

3,8-divinyl bacteriochlorophyllide a + NADPH + H+

Chlorobaculum tepidum TLS

-

bacteriochlorophyllide a + NADP+

-

Chlorobaculum tepidum

Q8KDI7

-

Chlorobaculum tepidum TLS

Q8KDI7

-

3,8-divinyl bacteriochlorophyllide a + NADPH + H+

-

745981

Chlorobaculum tepidum

bacteriochlorophyllide a + NADP+

-

3,8-divinyl bacteriochlorophyllide a + NADPH + H+

-

745981

Chlorobaculum tepidum TLS

bacteriochlorophyllide a + NADP+

-

NADPH

-

Chlorobaculum tepidum

NADPH

-

Chlorobaculum tepidum

additional information

construction of mutant tepdA lacking enzymes BciA and BchU, that catalyze reduction of the C8-vinyl group and methylation at the C20 position of bacteriochlorophyll (BChl) c, respectively, in the green sulfur bacterium Chlorobaculum tepidum, the mutant accumula C8-vinyl-bacteriochlorophyllide d derivative, determined by NMR to be (31R)-8-vinyl-12-ethyl-(R[V,E])BChl d. The bciA mutant possesses mainly (31R)-8-vinyl-12-ethyl-(R[V,E])BChl c as in chlorosomal self-aggregates in addition to minor (31R)-8-vinyl-12-methyl-(R[V,M]) and (31S)-8-vinyl-12-ethyl-(S[V,E])BChls c. Reconstitution of self-aggregates of C8V-BChl species in vitro. Comparison of chlorophyllide a derivatives in tepdA strains lacking bciA and/or bciU, overview

Chlorobaculum tepidum

3,8-divinyl bacteriochlorophyllide a + NADPH + H+

Chlorobaculum tepidum

-

bacteriochlorophyllide a + NADP+

-

3,8-divinyl bacteriochlorophyllide a + NADPH + H+

Chlorobaculum tepidum TLS

-

bacteriochlorophyllide a + NADP+

-

3,8-divinyl bacteriochlorophyllide a + NADPH + H+

-

745981

Chlorobaculum tepidum

bacteriochlorophyllide a + NADP+

-

3,8-divinyl bacteriochlorophyllide a + NADPH + H+

-

745981

Chlorobaculum tepidum TLS

bacteriochlorophyllide a + NADP+

-

malfunction

the bciA deletion mutant of Chlorobaculum tepidum accumulates C8V pigments. The bciA mutant possesses mainly (31R)-8-vinyl-12-ethyl-(R[V,E])BChl c as in chlorosomal self-aggregates in addition to minor (31R)-8-vinyl-12-methyl-(R[V,M]) and (31S)-8-vinyl-12-ethyl-(S[V,E])BChls c

Chlorobaculum tepidum

malfunction

the bciA deletion mutant of Chlorobaculum tepidum accumulates C8V pigments. The bciA mutant possesses mainly (31R)-8-vinyl-12-ethyl-(R[V,E])BChl c as in chlorosomal self-aggregates in addition to minor (31R)-8-vinyl-12-methyl-(R[V,M]) and (31S)-8-vinyl-12-ethyl-(S[V,E])BChls c

Chlorobaculum tepidum

745221

Chen

Two unrelated 8-vinyl reducta ...

Acaryochloris marina, Acaryochloris marina MBIC11017

J. Bacteriol.

198

1393-1400

2016

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1

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746442

Tsukatani

Rhodobacter sphaeroides mutan ...

Rhodobacter sphaeroides

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9741

2015

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J. Biol. Chem.

289

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Canniffe

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745981

Harada

Isolation and structural dete ...

Chlorobaculum tepidum, Chlorobaculum tepidum TLS

Photosyn. Res.

121

13-23

2014

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746037

Ito

Evolution of a new chlorophyl ...

Arabidopsis thaliana, Phaeodactylum tricornutum

Plant Cell Physiol.

55

593-603

2014

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746260

Tikh

A tale of two reductases exte ...

Chlorobaculum tepidum, Chlorobaculum tepidum TLS, Rhodobacter sphaeroides

PLoS ONE

9

e89734

2014

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746105

Wang

One divinyl reductase reduces ...

Arabidopsis thaliana, Cucumis sativus, Oryza sativa, Zea mays

Plant Physiol.

161

521-534

2013

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726217

Wang

Divinyl chlorophyll(ide) a can ...

Oryza sativa

Plant Physiol.

153

994-1003

2010

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739277

Nagata

The major route for chlorophyl ...

Arabidopsis thaliana

Plant Cell Physiol.

48

1803-1808

2007

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Chloroplast biogenesis 92: In ...

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1995

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Biochemistry

31

8460-8464

1992

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Chloroplast biogenesis 60. Con ...

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-

Isolation and structural determination of C8-vinyl-bacteriochlorophyll d from the bciA and bchU double mutant of the green sulfur bacterium Chlorobaculum tepidum

Data extracted from this reference: