Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of mutant tepdA lacking enzymes BciA and BchU, that catalyze reduction of the C8-vinyl group and methylation at the C20 position of bacteriochlorophyll (BChl) c, respectively, in the green sulfur bacterium Chlorobaculum tepidum, the mutant accumula C8-vinyl-bacteriochlorophyllide d derivative, determined by NMR to be (31R)-8-vinyl-12-ethyl-(R[V,E])BChl d. The bciA mutant possesses mainly (31R)-8-vinyl-12-ethyl-(R[V,E])BChl c as in chlorosomal self-aggregates in addition to minor (31R)-8-vinyl-12-methyl-(R[V,M]) and (31S)-8-vinyl-12-ethyl-(S[V,E])BChls c. Reconstitution of self-aggregates of C8V-BChl species in vitro. Comparison of chlorophyllide a derivatives in tepdA strains lacking bciA and/or bciU, overview | Chlorobaculum tepidum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,8-divinyl bacteriochlorophyllide a + NADPH + H+ | Chlorobaculum tepidum | - |
bacteriochlorophyllide a + NADP+ | - |
? | |
3,8-divinyl bacteriochlorophyllide a + NADPH + H+ | Chlorobaculum tepidum TLS | - |
bacteriochlorophyllide a + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorobaculum tepidum | Q8KDI7 | - |
- |
Chlorobaculum tepidum TLS | Q8KDI7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,8-divinyl bacteriochlorophyllide a + NADPH + H+ | - |
Chlorobaculum tepidum | bacteriochlorophyllide a + NADP+ | - |
? | |
3,8-divinyl bacteriochlorophyllide a + NADPH + H+ | - |
Chlorobaculum tepidum TLS | bacteriochlorophyllide a + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BciA | - |
Chlorobaculum tepidum |
C8V reductase | - |
Chlorobaculum tepidum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Chlorobaculum tepidum |
General Information | Comment | Organism |
---|---|---|
malfunction | the bciA deletion mutant of Chlorobaculum tepidum accumulates C8V pigments. The bciA mutant possesses mainly (31R)-8-vinyl-12-ethyl-(R[V,E])BChl c as in chlorosomal self-aggregates in addition to minor (31R)-8-vinyl-12-methyl-(R[V,M]) and (31S)-8-vinyl-12-ethyl-(S[V,E])BChls c | Chlorobaculum tepidum |