additional information
construction of mutant tepdA lacking enzymes BciA and BchU, that catalyze reduction of the C8-vinyl group and methylation at the C20 position of bacteriochlorophyll (BChl) c, respectively, in the green sulfur bacterium Chlorobaculum tepidum, the mutant accumula C8-vinyl-bacteriochlorophyllide d derivative, determined by NMR to be (31R)-8-vinyl-12-ethyl-(R[V,E])BChl d. The bciA mutant possesses mainly (31R)-8-vinyl-12-ethyl-(R[V,E])BChl c as in chlorosomal self-aggregates in addition to minor (31R)-8-vinyl-12-methyl-(R[V,M]) and (31S)-8-vinyl-12-ethyl-(S[V,E])BChls c. Reconstitution of self-aggregates of C8V-BChl species in vitro. Comparison of chlorophyllide a derivatives in tepdA strains lacking bciA and/or bciU, overview
Chlorobaculum tepidum
3,8-divinyl bacteriochlorophyllide a + NADPH + H+
Chlorobaculum tepidum
bacteriochlorophyllide a + NADP+
?
3,8-divinyl bacteriochlorophyllide a + NADPH + H+
Chlorobaculum tepidum TLS
bacteriochlorophyllide a + NADP+
?
3,8-divinyl bacteriochlorophyllide a + NADPH + H+
745981
Chlorobaculum tepidum
bacteriochlorophyllide a + NADP+
?
3,8-divinyl bacteriochlorophyllide a + NADPH + H+
745981
Chlorobaculum tepidum TLS
bacteriochlorophyllide a + NADP+
?
additional information
construction of mutant tepdA lacking enzymes BciA and BchU, that catalyze reduction of the C8-vinyl group and methylation at the C20 position of bacteriochlorophyll (BChl) c, respectively, in the green sulfur bacterium Chlorobaculum tepidum, the mutant accumula C8-vinyl-bacteriochlorophyllide d derivative, determined by NMR to be (31R)-8-vinyl-12-ethyl-(R[V,E])BChl d. The bciA mutant possesses mainly (31R)-8-vinyl-12-ethyl-(R[V,E])BChl c as in chlorosomal self-aggregates in addition to minor (31R)-8-vinyl-12-methyl-(R[V,M]) and (31S)-8-vinyl-12-ethyl-(S[V,E])BChls c. Reconstitution of self-aggregates of C8V-BChl species in vitro. Comparison of chlorophyllide a derivatives in tepdA strains lacking bciA and/or bciU, overview
Chlorobaculum tepidum
3,8-divinyl bacteriochlorophyllide a + NADPH + H+
Chlorobaculum tepidum
bacteriochlorophyllide a + NADP+
?
3,8-divinyl bacteriochlorophyllide a + NADPH + H+
Chlorobaculum tepidum TLS
bacteriochlorophyllide a + NADP+
?
3,8-divinyl bacteriochlorophyllide a + NADPH + H+
745981
Chlorobaculum tepidum
bacteriochlorophyllide a + NADP+
?
3,8-divinyl bacteriochlorophyllide a + NADPH + H+
745981
Chlorobaculum tepidum TLS
bacteriochlorophyllide a + NADP+
?
745221
Chen
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Harada
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Ito
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Tikh
A tale of two reductases ext ...
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Wang
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Divinyl chlorophyll(ide) a can ...
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