Reference to 1.3.1.75; Id = 745981 - BRENDA Enzyme Database

additional information

construction of mutant tepdA lacking enzymes BciA and BchU, that catalyze reduction of the C8-vinyl group and methylation at the C20 position of bacteriochlorophyll (BChl) c, respectively, in the green sulfur bacterium Chlorobaculum tepidum, the mutant accumula C8-vinyl-bacteriochlorophyllide d derivative, determined by NMR to be (31R)-8-vinyl-12-ethyl-(R[V,E])BChl d. The bciA mutant possesses mainly (31R)-8-vinyl-12-ethyl-(R[V,E])BChl c as in chlorosomal self-aggregates in addition to minor (31R)-8-vinyl-12-methyl-(R[V,M]) and (31S)-8-vinyl-12-ethyl-(S[V,E])BChls c. Reconstitution of self-aggregates of C8V-BChl species in vitro. Comparison of chlorophyllide a derivatives in tepdA strains lacking bciA and/or bciU, overview

Chlorobaculum tepidum

3,8-divinyl bacteriochlorophyllide a + NADPH + H+

Chlorobaculum tepidum

-

bacteriochlorophyllide a + NADP+

-

?

3,8-divinyl bacteriochlorophyllide a + NADPH + H+

Chlorobaculum tepidum TLS

-

bacteriochlorophyllide a + NADP+

-

?

Chlorobaculum tepidum

Q8KDI7

-

Chlorobaculum tepidum TLS

Q8KDI7

-

3,8-divinyl bacteriochlorophyllide a + NADPH + H+

-

745981

Chlorobaculum tepidum

bacteriochlorophyllide a + NADP+

-

?

3,8-divinyl bacteriochlorophyllide a + NADPH + H+

-

745981

Chlorobaculum tepidum TLS

bacteriochlorophyllide a + NADP+

-

?

BciA

-

Chlorobaculum tepidum

C8V reductase

-

Chlorobaculum tepidum

NADPH

-

Chlorobaculum tepidum

NADPH

-

Chlorobaculum tepidum

additional information

construction of mutant tepdA lacking enzymes BciA and BchU, that catalyze reduction of the C8-vinyl group and methylation at the C20 position of bacteriochlorophyll (BChl) c, respectively, in the green sulfur bacterium Chlorobaculum tepidum, the mutant accumula C8-vinyl-bacteriochlorophyllide d derivative, determined by NMR to be (31R)-8-vinyl-12-ethyl-(R[V,E])BChl d. The bciA mutant possesses mainly (31R)-8-vinyl-12-ethyl-(R[V,E])BChl c as in chlorosomal self-aggregates in addition to minor (31R)-8-vinyl-12-methyl-(R[V,M]) and (31S)-8-vinyl-12-ethyl-(S[V,E])BChls c. Reconstitution of self-aggregates of C8V-BChl species in vitro. Comparison of chlorophyllide a derivatives in tepdA strains lacking bciA and/or bciU, overview

Chlorobaculum tepidum

3,8-divinyl bacteriochlorophyllide a + NADPH + H+

Chlorobaculum tepidum

-

bacteriochlorophyllide a + NADP+

-

?

3,8-divinyl bacteriochlorophyllide a + NADPH + H+

Chlorobaculum tepidum TLS

-

bacteriochlorophyllide a + NADP+

-

?

3,8-divinyl bacteriochlorophyllide a + NADPH + H+

-

745981

Chlorobaculum tepidum

bacteriochlorophyllide a + NADP+

-

?

3,8-divinyl bacteriochlorophyllide a + NADPH + H+

-

745981

Chlorobaculum tepidum TLS

bacteriochlorophyllide a + NADP+

-

?

malfunction

the bciA deletion mutant of Chlorobaculum tepidum accumulates C8V pigments. The bciA mutant possesses mainly (31R)-8-vinyl-12-ethyl-(R[V,E])BChl c as in chlorosomal self-aggregates in addition to minor (31R)-8-vinyl-12-methyl-(R[V,M]) and (31S)-8-vinyl-12-ethyl-(S[V,E])BChls c

Chlorobaculum tepidum

malfunction

the bciA deletion mutant of Chlorobaculum tepidum accumulates C8V pigments. The bciA mutant possesses mainly (31R)-8-vinyl-12-ethyl-(R[V,E])BChl c as in chlorosomal self-aggregates in addition to minor (31R)-8-vinyl-12-methyl-(R[V,M]) and (31S)-8-vinyl-12-ethyl-(S[V,E])BChls c

Chlorobaculum tepidum

745221

Chen

Two unrelated 8-vinyl reducta ...

Acaryochloris marina, Acaryochloris marina MBIC11017

J. Bacteriol.

198

1393-1400

2016

-

1

-

2

-

7

-

2

1

6

-

1

-

1

-

2

-

2

1

-

4

-

746442

Tsukatani

Rhodobacter sphaeroides mutan ...

Luteovulum sphaeroides

Sci. Rep.

5

9741

2015

-

1

-

6

-

2

-

1

-

1

-

736444

Harada

Chlorophyllide a oxidoreductas ...

Luteovulum sphaeroides, Rhodopseudomonas palustris, Luteovulum sphaeroides J001, Rhodopseudomonas palustris J002

J. Biol. Chem.

289

12716-12726

2014

-

2

-

2

-

5

-

7

-

5

-

7

-

2

-

2

-

2

-

5

-

5

-

4

-

744270

Canniffe

Elucidation of the preferred ...

Luteovulum sphaeroides, Luteovulum sphaeroides DSM 158

Biochem. J.

462

433-440

2014

-

1

-

4

-

8

-

6

-

7

-

1

-

1

-

4

-

6

-

1

2

1

-

745981

Harada

Isolation and structural dete ...

Chlorobaculum tepidum, Chlorobaculum tepidum TLS

Photosynth. Res.

121

13-23

2014

-

1

-

2

-

3

-

2

-

2

-

1

-

1

-

1

-

2

-

2

-

1

-

746037

Ito

Evolution of a new chlorophyl ...

Phaeodactylum tricornutum, Arabidopsis thaliana

Plant Cell Physiol.

55

593-603

2014

-

2

-

3

-

4

-

7

-

10

1

-

1

-

2

-

2

-

3

-

7

-

1

-

1

-

4

-

746260

Tikh

A tale of two reductases ext ...

Luteovulum sphaeroides, Chlorobaculum tepidum, Chlorobaculum tepidum TLS, Luteovulum sphaeroides 2.4.1

PLoS ONE

9

e89734

2014

-

2

-

2

-

6

-

9

-

2

-

10

-

12

2

-

2

-

2

-

2

-

2

-

6

-

2

-

10

-

2

-

2

-

1

-

746105

Wang

One divinyl reductase reduces ...

Zea mays, Cucumis sativus, Oryza sativa, Arabidopsis thaliana

Plant Physiol.

161

521-534

2013

-

4

-

1

-

5

-

10

-

12

-

6

-

14

2

13

4

-

4

-

4

-

4

-

1

-

5

-

10

-

6

-

14

2

4

-

4

-

9

-

726217

Wang

Divinyl chlorophyll(ide) a can ...

Oryza sativa

Plant Physiol.

153

994-1003

2010

-

2

-

13

-

1

-

3

-

5

1

-

1

-

1

-

2

1

-

1

-

3

-

1

-

1

-

1

-

739277

Nagata

The major route for chlorophyl ...

Arabidopsis thaliana

Plant Cell Physiol.

48

1803-1808

2007

-

1

-

1

-

2

-

2

-

3

1

-

1

-

2

-

1

2

-

1

-

2

-

1

-

1

-

667191

Kolossov

Chloroplast biogenesis 92: In ...

Cucumis sativus

Anal. Biochem.

348

192-197

2006

-

3

-

1

-

639381

Kolossov

Chloroplast biogenesis 84: sol ...

Cucumis sativus, Hordeum vulgare

Anal. Biochem.

295

214-219

2001

-

1

-

7

-

2

-

4

-

1

-

2

1

4

-

1

-

2

-

1

-

7

-

2

-

1

-

2

1

4

-

1

-

2

-

639380

Parham

Chloroplast biogenesis 72: a [ ...

Cucumis sativus, Hordeum vulgare, Zea mays

Anal. Biochem.

231

164-169

1995

-

9

-

3

-

7

-

6

1

-

5

-

1

-

3

1

-

9

-

3

-

6

1

-

5

-

1

-

3

1

-

639379

Parham

Chloroplast biogenesis: [4-vin ...

Cucumis sativus, Hordeum vulgare

Biochemistry

31

8460-8464

1992

-

6

-

2

-

3

-

1

-

2

-

4

-

6

-

2

-

1

-

2

-

4

-

639378

Tripathy

Chloroplast biogenesis 60. Con ...

Cucumis sativus, Hordeum vulgare

Plant Physiol.

87

89-94

1988

-

3

-

2

-

2

-

Isolation and structural determination of C8-vinyl-bacteriochlorophyll d from the bciA and bchU double mutant of the green sulfur bacterium Chlorobaculum tepidum

Data extracted from this reference: