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Literature summary for 1.3.1.75 extracted from

  • Chen, G.E.; Hitchcock, A.; Jackson, P.J.; Chaudhuri, R.R.; Dickman, M.J.; Hunter, C.N.; Canniffe, D.P.
    Two unrelated 8-vinyl reductases ensure production of mature chlorophylls in Acaryochloris marina (2016), J. Bacteriol., 198, 1393-1400 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.75

Cloned(Commentary)

Cloned (Comment) Organism
gene bciA or nmrA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, nmrA is introduced into an 8-vinyl chlorophyll a-producing DELTAbciB strain of Synechocystis sp. strain PCC 6803, and restores synthesis of the pigment with an ethyl group at C-8, demonstrating its activity as 8-vinyl reductase, subcloning in Escherichia coli strain JM109 Acaryochloris marina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,8-divinyl chlorophyllide a + NADPH + H+ Acaryochloris marina
-
 chlorophyllide a + NADP+
-
 ?
3,8-divinyl chlorophyllide a + NADPH + H+ Acaryochloris marina MBIC11017
-
 chlorophyllide a + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Acaryochloris marina B0C357
-
-
Acaryochloris marina MBIC11017 B0C357
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,8-divinyl chlorophyllide a + NADPH + H+
-
 Acaryochloris marina chlorophyllide a + NADP+
-
 ?
3,8-divinyl chlorophyllide a + NADPH + H+
-
 Acaryochloris marina MBIC11017 chlorophyllide a + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 36780, sequence calculation Acaryochloris marina

Synonyms

Synonyms Comment Organism
8-vinyl reductase
-
 Acaryochloris marina
8VR
-
 Acaryochloris marina
BciA
-
 Acaryochloris marina
BciA-type 8VR
-
 Acaryochloris marina
nmrA
-
 Acaryochloris marina

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Acaryochloris marina

General Information

General Information Comment Organism
evolution two unrelated classes of 8-vinyl reductases are known to exist in oxygenic phototrophs, BciA and BciB. Transcript and proteomic analysis of Acaryochloris marina reveal that both bciA and bciB are expressed and their encoded proteins are present in the cell, possibly in order to ensure that all synthesized chlorophyll pigment carries an ethyl group at C-8. The presence of two 8-vinyl reductases is unique for cyanobacteria. The phylogenetic positions of Acaryochloris marina BciA and BciB are both broadly consistent with those shown for Acaryochloris marina in the 16S rRNA trees, suggesting that the bciA and bciB genes have not been acquired by horizontal transfer. However, the positions of Synechococcus spp. in the BciA tree and the clade containing the green sulfur bacteria in the BciB tree are inconsistent with the 16S rRNA phylogeny, indicating that there may have been lateral transfer events during the evolution of both bciA and bciB Acaryochloris marina
malfunction loss of 8-vinyl reductase activity in Acaryochloris marina results in the production of 8-vinyl-Chl a and 8-vinyl-Chl d with a negative effect on viability of the cells Acaryochloris marina
additional information the enzyme encoded by gene nmrA shows 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (NADPH) activity and shall be renamed bciA Acaryochloris marina
physiological function functional complementation of an enzyme-deficient DELTAbciB Synechocystis sp. strain PCC 6803 Acaryochloris marina