Literature summary for 1.3.1.74 extracted from

  • Mano, J.; Belles-Boix, E.; Babiychuk, E.; Inze, D.; Torii, Y.; Hiraoka, E.; Takimoto, K.; Slooten, L.; Asada, K.; Kushnir, S.
    Protection against photooxidative injury of tobacco leaves by 2-alkenal reductase. Detoxication of lipid peroxide-derived reactive carbonyls (2005), Plant Physiol., 139, 1773-1783.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression of At-AER in tobacco suppresses the damages of leaf tissue by the exogenously added reactive carbonyl 4-hydroxy-(2E)-nonenal. The leaves of At-AER overproducing tobaccos suffer less damage caused by photooxidative stress due to MV/light or to intense light only Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
9-oxo-(10E,12Z)-octadecadienoic acid pH 6.0 Arabidopsis thaliana
0.0134
-
4-hydroxy-(2E)-nonenal pH 6.0 Arabidopsis thaliana
0.0113
-
(2E,6Z)-nonadienal pH 6.0 Arabidopsis thaliana
0.00124
-
4-oxo-(2E)-nonenal pH 6.0 Arabidopsis thaliana
0.00392
-
13-oxo-(9E,11Z)-octadecadienoic acid pH 6.0 Arabidopsis thaliana
0.000673
-
3-nonen-2-one pH 6.0 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol At-AER protein fused with the Aequorea victoria green fluorescent protein localizes in cytosol and the nucleus in Bright-Yellow 2 cells of tobacco Arabidopsis thaliana
-
-
nucleus At-AER protein fused with the Aequorea victoria green fluorescent protein localizes in cytosol and the nucleus in Bright-Yellow 2 cells of tabacco Arabidopsis thaliana
-
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E),(6Z)-nonadienal + NADPH
-
Arabidopsis thaliana ?
-
?
13-oxo-(9E,11Z)-octadecadienoic acid + NADPH
-
Arabidopsis thaliana ?
-
?
3-nonen-2-one + NADPH
-
Arabidopsis thaliana nonan-2-one
-
?
4-hydroxy-(2E)-nonenal + NADPH + H+
-
Arabidopsis thaliana 4-hydroxynonanal + NADP+
-
?
4-oxo-(2E)-nonenal + NADPH + H+
-
Arabidopsis thaliana 4-oxononanal + NADP+
-
?
9-oxo-(10E,12Z)-octadecadienoic acid + NADPH
-
Arabidopsis thaliana ?
-
?

Synonyms

Synonyms Comment Organism
At-AER
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1
-
13-oxo-(9E,11Z)-octadecadienoic acid pH 6.0 Arabidopsis thaliana
20 50 4-hydroxy-(2E)-nonenal pH 6.0 Arabidopsis thaliana
20.1
-
4-oxo-(2E)-nonenal pH 6.0 Arabidopsis thaliana
18.9
-
3-nonen-2-one pH 6.0 Arabidopsis thaliana
0.94
-
9-oxo-(10E,12Z)-octadecadienoic acid pH 6.0 Arabidopsis thaliana
33.9
-
(2E,6Z)-nonadienal pH 6.0 Arabidopsis thaliana
88.1
-
4-hydroxy-(2E)-nonenal pH 6.0 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Arabidopsis thaliana