Literature summary for 1.3.1.72 extracted from

Lu, X.; Jia, D.; Zhao, C.; Wang, W.; Liu, T.; Chen, S.; Quan, X.; Sun, D.; Gao, B.
Recombinant adenovirus-mediated overexpression of 3beta-hydroxysteroid-DELTA24 reductase (2014), Neural Regen. Res., 9, 504-512 .

Application

Application	Comment	Organism
medicine	DHCR24 gene therapy might be useful for treatment of Alzheimer's disease	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
construction of a recombinant adenovirus driving DHCR24 expression vector specific for neurons with neuron-specific promoter, synapsin-1 (SYN1), cloning of a SYN1 promoter DNA fragment of human enzyme DHCR24, and transfection of recombinant Ad-hSYN1-DHCR24 into HEK AD-293, N2A (mouse neuroblastoma), and MIN6 (mouse pancreatic carcinoma) cells. DHCR24 is specially expressed in HEK AD-293 and N2A cells, but not in MIN6 cells. Adenovirus transfection inhibits apoptosis through scavenging excess reactive oxygen species, and recombinant DHCR24 adenoviruse induces neuron-specific DHCR24 expression	Homo sapiens
construction of a recombinant adenovirus driving DHCR24 expression vector specific for neurons with neuron-specific promoter, synapsin-1 (SYN1), cloning of a SYN1 promoter DNA fragment of rat enzyme DHCR24, and transfection of recombinant Ad-rSYN1-DHCR24 into HEK AD-293, N2A (mouse neuroblastoma), and MIN6 (mouse pancreatic carcinoma) cells. DHCR24 is specially expressed in HEK AD-293 and N2A cells, but not in MIN6 cells. Adenovirus transfection inhibits apoptosis through scavenging excess reactive oxygen species, and recombinant DHCR24 adenoviruse induces neuron-specific DHCR24 expression	Rattus norvegicus

Cloned (Comment)

Organism

construction of a recombinant adenovirus driving DHCR24 expression vector specific for neurons with neuron-specific promoter, synapsin-1 (SYN1), cloning of a SYN1 promoter DNA fragment of human enzyme DHCR24, and transfection of recombinant Ad-hSYN1-DHCR24 into HEK AD-293, N2A (mouse neuroblastoma), and MIN6 (mouse pancreatic carcinoma) cells. DHCR24 is specially expressed in HEK AD-293 and N2A cells, but not in MIN6 cells. Adenovirus transfection inhibits apoptosis through scavenging excess reactive oxygen species, and recombinant DHCR24 adenoviruse induces neuron-specific DHCR24 expression

Homo sapiens

construction of a recombinant adenovirus driving DHCR24 expression vector specific for neurons with neuron-specific promoter, synapsin-1 (SYN1), cloning of a SYN1 promoter DNA fragment of rat enzyme DHCR24, and transfection of recombinant Ad-rSYN1-DHCR24 into HEK AD-293, N2A (mouse neuroblastoma), and MIN6 (mouse pancreatic carcinoma) cells. DHCR24 is specially expressed in HEK AD-293 and N2A cells, but not in MIN6 cells. Adenovirus transfection inhibits apoptosis through scavenging excess reactive oxygen species, and recombinant DHCR24 adenoviruse induces neuron-specific DHCR24 expression

Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum	-	Homo sapiens	5783	-
endoplasmic reticulum	-	Rattus norvegicus	5783	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q15392	-	-
Rattus norvegicus	Q5BQE6	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Homo sapiens	-
brain	-	Rattus norvegicus	-
colon	-	Homo sapiens	-
colon	-	Rattus norvegicus	-
heart	-	Homo sapiens	-
heart	-	Rattus norvegicus	-
liver	-	Homo sapiens	-
liver	-	Rattus norvegicus	-
lung	-	Homo sapiens	-
lung	-	Rattus norvegicus	-

Synonyms

Synonyms	Comment	Organism
3beta-hydroxysteroid-DELTA24 reductase	-	Homo sapiens
3beta-hydroxysteroid-DELTA24 reductase	-	Rattus norvegicus
DHCR24	-	Homo sapiens
DHCR24	-	Rattus norvegicus

General Information

General Information	Comment	Organism
physiological function	the enzyme has neuroprotective and cholesterol-synthesizing activities. DHCR24 overexpression confers neuroprotection against apoptosis caused by amyloid beta deposition	Homo sapiens
physiological function	the enzyme has neuroprotective and cholesterol-synthesizing activities. DHCR24 overexpression confers neuroprotection against apoptosis caused by amyloid beta deposition	Rattus norvegicus