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Literature summary for 1.3.1.72 extracted from

  • Zerenturk, E.J.; Sharpe, L.J.; Brown, A.J.
    DHCR24 associates strongly with the endoplasmic reticulum beyond predicted membrane domains implications for the activities of this multi-functional enzyme (2014), Biosci. Rep., 34, e00098 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.72

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of truncated DHCR24 (DELTA23 DHCR24) with a V5 epitope tag at either the N-terminus (V5-DHCR24) or C-terminus (DHCR24-V5) in CHO-7 cells, and the cellular localization of DELTA23 DHCR24 is primarily to the membrane fraction like the wild-type Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of a truncated DHCR24, DELTA23 DHCR24, lacking the secretory signal peptide Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane DHCR24 associates strongly with the endoplasmic reticulum beyond predicted membrane domains. The majority of the enzyme is associated with the endoplasmic reticulum membrane. The predicted membrane topology of DHCR24 consists of N-terminal transmembrane domains. The putative transmembrane domain is not essential for DHCR24 membrane association Homo sapiens 5789
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additional information enzyme DHCR24 is not predicted to associate with the membrane via post-translational modifications. The enzyme sequence contains a secretory signal peptide and an extremely hydrophobic N-terminus, hydrophobicity profile overview Homo sapiens
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesta-5,24-dien-3beta-ol + NADPH + H+ Homo sapiens reduction of desmosterol to cholesterol is dependent on FAD cholest-5-en-3beta-ol + NADP+
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q15392
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Purification (Commentary)

Purification (Comment) Organism
partially from transgenic CHO cells by subcellular fractionation and membrane isolation Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesta-5,24-dien-3beta-ol + NADPH + H+ reduction of desmosterol to cholesterol is dependent on FAD Homo sapiens cholest-5-en-3beta-ol + NADP+
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 ?

Synonyms

Synonyms Comment Organism
3beta-hydroxysterol DELTA24-reductase
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 Homo sapiens
DHCR24
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 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD DHCR24 contains a highly conserved FAD (flavin adenine dinucleotide)-binding domain Homo sapiens
NADPH
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 Homo sapiens