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Literature summary for 1.3.1.72 extracted from

  • Nishimura, T.; Kohara, M.; Izumi, K.; Kasama, Y.; Hirata, Y.; Huang, Y.; Shuda, M.; Mukaidani, C.; Takano, T.; Tokunaga, Y.; Nuriya, H.; Satoh, M.; Saito, M.; Kai, C.; Tsukiyama-Kohara, K.
    Hepatitis C virus impairs p53 via persistent overexpression of 3beta-hydroxysterol DELTA24-reductase (2009), J. Biol. Chem., 284, 36442-36452.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
RzM6-0d cells are transduced with DHCR24 lentivirus. Expression of DHCR24 from pGEM-T easy vector via in vitro translation using reticulocyte lysate. Expression of HA- or FLAG-tagged DHCR24 in RzM6 or HepG2 cells. Expression of GFP- and FLAG-tagged DHCR24 in 293FT cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information silencing of DHCR24 and HCV by siRNA Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
Hep-G2 cell
-
 Homo sapiens
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Huh-7 cell
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 Homo sapiens
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RzM6-LC cell a human hepatoblastoma-derived cell line Homo sapiens
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Synonyms

Synonyms Comment Organism
3beta-hydroxysterol DELTA24-reductase
-
 Homo sapiens
DHCR24
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 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens hepatitis C virus induces the DHCR24 overexpression in human hepatocytes up

General Information

General Information Comment Organism
additional information the hepatitis C virus-expressing hepatoblastoma-derived cell line, RzM6-LC, shows augmented tumorigenicity. DHCR24 expression reflects tumorigenicity, overview. Ectopic or HCV-induced DHCR24 overexpression results in resistance to oxidative stress-induced apoptosis and suppressed p53 activity. DHCR24 overexpression in these cells paralleles the increased interaction between p53 and MDM2, a p53-specific E3 ubiquitin ligase, in the cytoplasm. Persistent DHCR24 overexpression does not alter the phosphorylation status of p53 but results in decreased acetylation of p53 at Lys residues 373 and 382 in the nucleus after treatment with H2O2. DHCR24 overexpression inhibits polyubiquitination in RzM6-LC cells and H358, p53 null, cells. Ectopic expression of DHCR24 does not inhibit apoptotic response to H2O2 in WI38 cells Homo sapiens