BRENDA - Enzyme Database show
show all sequences of 1.3.1.7

Substrate determinants of the course of tartrate dehydrogenase-catalyzed reactions

Serfozo, P.; Tipton, P.A.; Biochemistry 34, 7517-7524 (1995)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene encoding TDH cloned into the pET3a expression vector
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.011
-
(2R,3S)-3-bromomalate
-
Escherichia coli
0.012
-
(2R,3S)-3-chloromalate
-
Escherichia coli
0.019
-
(2R,3S)-3-fluoromalate
-
Escherichia coli
0.024
-
(2R,3R)-3-iodomalate
-
Escherichia coli
0.027
-
(2R,3S)-3-iodomalate
-
Escherichia coli
0.05
-
D-malate
-
Escherichia coli
0.06
-
(2R,3R)-3-methyltartrate
-
Escherichia coli
0.06
-
meso-tartrate
-
Escherichia coli
0.07
-
(2R,3S)-3-methyltartrate
-
Escherichia coli
0.1
-
(+)-tartrate
-
Escherichia coli
0.22
-
(2R,3R)-3-bromomalate
-
Escherichia coli
0.27
-
(2R,3R)-3-chloromalate
-
Escherichia coli
0.67
-
(2R,3R)-3-fluoromalate
-
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Pseudomonas putida
-
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2R,3R)-3-bromomalate + NAD+
oxidative decarboxylation
349359
Escherichia coli
? + CO2 + NADH
-
-
-
?
(2R,3R)-3-chloromalate + NAD+
oxidative decarboxylation
349359
Escherichia coli
? + CO2 + NADH
-
-
-
?
(2R,3R)-3-fluoromalate + NAD+
oxidative decarboxylation
349359
Escherichia coli
? + CO2 + NADH
-
-
-
?
(2R,3R)-3-iodomalate + NAD+
oxidative decarboxylation
349359
Escherichia coli
? + CO2 + NADH
-
-
-
?
(2R,3R)-3-methyltartrate + NAD+
oxidative decarboxylation
349359
Escherichia coli
? + CO2 + NADH
-
-
-
?
(2R,3R)-3-thiomalate + NAD+
-
349359
Escherichia coli
?
-
-
-
?
(2R,3S)-3-bromomalate + NAD+
oxidative decarboxylation
349359
Escherichia coli
? + CO2 + NADH
-
-
-
?
(2R,3S)-3-chloromalate + NAD+
oxidative decarboxylation
349359
Escherichia coli
? + CO2 + NADH
-
-
-
?
(2R,3S)-3-fluoromalate + NAD+
oxidative decarboxylation
349359
Escherichia coli
? + CO2 + NADH
-
-
-
?
(2R,3S)-3-iodomalate + NAD+
oxidative decarboxylation
349359
Escherichia coli
? + CO2 + NADH
-
-
-
?
(2R,3S)-3-methyltartrate + NAD+
simple oxidation
349359
Escherichia coli
? + NADH
-
-
-
?
(2R,3S)-3-thiomalate + NAD+
net non-oxidative decarboxylation
349359
Escherichia coli
?
-
-
-
?
L-(+)-tartrate + NAD+
-
349359
Pseudomonas putida
(3R)-oxaloglycolate + NADH
-
-
-
?
L-(+)-tartrate + NAD+
in presence of Mn2+ and K+ (+)-tartrate is subject to NAD+-dependent oxidation to form oxaloglycolate
349359
Escherichia coli
(3R)-oxaloglycolate + NADH
-
349359
Escherichia coli
?
malate + NAD+
-
349359
Escherichia coli
pyruvate + CO2 + NADH
-
349359
Escherichia coli
?
meso-tartrate + NAD+
-
349359
Escherichia coli
D-glycerate + CO2 + NADH
-
-
-
?
additional information
3R-oxaloglycolate, (2R,3S)-3-aminomalate and (2R,3R)-3-aminomalate are no substrates
349359
Escherichia coli
?
-
-
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
gene encoding TDH cloned into the pET3a expression vector
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.011
-
(2R,3S)-3-bromomalate
-
Escherichia coli
0.012
-
(2R,3S)-3-chloromalate
-
Escherichia coli
0.019
-
(2R,3S)-3-fluoromalate
-
Escherichia coli
0.024
-
(2R,3R)-3-iodomalate
-
Escherichia coli
0.027
-
(2R,3S)-3-iodomalate
-
Escherichia coli
0.05
-
D-malate
-
Escherichia coli
0.06
-
(2R,3R)-3-methyltartrate
-
Escherichia coli
0.06
-
meso-tartrate
-
Escherichia coli
0.07
-
(2R,3S)-3-methyltartrate
-
Escherichia coli
0.1
-
(+)-tartrate
-
Escherichia coli
0.22
-
(2R,3R)-3-bromomalate
-
Escherichia coli
0.27
-
(2R,3R)-3-chloromalate
-
Escherichia coli
0.67
-
(2R,3R)-3-fluoromalate
-
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2R,3R)-3-bromomalate + NAD+
oxidative decarboxylation
349359
Escherichia coli
? + CO2 + NADH
-
-
-
?
(2R,3R)-3-chloromalate + NAD+
oxidative decarboxylation
349359
Escherichia coli
? + CO2 + NADH
-
-
-
?
(2R,3R)-3-fluoromalate + NAD+
oxidative decarboxylation
349359
Escherichia coli
? + CO2 + NADH
-
-
-
?
(2R,3R)-3-iodomalate + NAD+
oxidative decarboxylation
349359
Escherichia coli
? + CO2 + NADH
-
-
-
?
(2R,3R)-3-methyltartrate + NAD+
oxidative decarboxylation
349359
Escherichia coli
? + CO2 + NADH
-
-
-
?
(2R,3R)-3-thiomalate + NAD+
-
349359
Escherichia coli
?
-
-
-
?
(2R,3S)-3-bromomalate + NAD+
oxidative decarboxylation
349359
Escherichia coli
? + CO2 + NADH
-
-
-
?
(2R,3S)-3-chloromalate + NAD+
oxidative decarboxylation
349359
Escherichia coli
? + CO2 + NADH
-
-
-
?
(2R,3S)-3-fluoromalate + NAD+
oxidative decarboxylation
349359
Escherichia coli
? + CO2 + NADH
-
-
-
?
(2R,3S)-3-iodomalate + NAD+
oxidative decarboxylation
349359
Escherichia coli
? + CO2 + NADH
-
-
-
?
(2R,3S)-3-methyltartrate + NAD+
simple oxidation
349359
Escherichia coli
? + NADH
-
-
-
?
(2R,3S)-3-thiomalate + NAD+
net non-oxidative decarboxylation
349359
Escherichia coli
?
-
-
-
?
L-(+)-tartrate + NAD+
-
349359
Pseudomonas putida
(3R)-oxaloglycolate + NADH
-
-
-
?
L-(+)-tartrate + NAD+
in presence of Mn2+ and K+ (+)-tartrate is subject to NAD+-dependent oxidation to form oxaloglycolate
349359
Escherichia coli
(3R)-oxaloglycolate + NADH
-
349359
Escherichia coli
?
malate + NAD+
-
349359
Escherichia coli
pyruvate + CO2 + NADH
-
349359
Escherichia coli
?
meso-tartrate + NAD+
-
349359
Escherichia coli
D-glycerate + CO2 + NADH
-
-
-
?
additional information
3R-oxaloglycolate, (2R,3S)-3-aminomalate and (2R,3R)-3-aminomalate are no substrates
349359
Escherichia coli
?
-
-
-
-
Other publictions for EC 1.3.1.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
349359
Serfozo
Substrate determinants of the ...
Escherichia coli, Pseudomonas putida
Biochemistry
34
7517-7524
1995
-
-
1
-
-
-
-
13
-
-
-
-
-
2
-
-
1
-
-
-
-
-
17
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
1
-
-
-
-
17
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349358
Beecher
Tartrate dehydrogenase-oxalate ...
Escherichia coli
Arch. Biochem. Biophys.
315
255-261
1994
-
-
1
-
-
-
1
8
-
-
-
-
-
1
-
-
1
1
-
-
-
-
4
-
-
-
-
2
-
-
-
4
-
-
-
-
-
1
4
-
-
-
-
1
-
8
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
287509
Do Nascimento
The stereospecificity of seque ...
Pseudomonas putida, Pseudomonas putida ATCC 17642
Biochem. J.
149
553-557
1975
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349356
Kohn
-
L- and mesotartaric acid dehyd ...
Bos taurus, Pseudomonas putida, Pseudomonas sp., Pseudomonas sp. A, Rattus norvegicus
Methods Enzymol.
9
236-240
1966
1
-
-
1
-
-
-
4
2
1
-
3
-
9
-
-
1
-
-
-
1
-
5
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
4
2
1
-
3
-
-
-
1
-
-
1
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-