Data extracted from this reference:
Cloned(Commentary)
Escherichia coli cells containing the plasmid pTDH1 which contains the gene encoding TDH under the control of the T7 polymerase promoter
Escherichia coli
Inhibitors
oxalate
time-dependent inhibition
Escherichia coli
KM Value [mM]
0.032
D-malate
thio-NAD+ as coenzyme
Escherichia coli
0.049
D-malate
NAD+ as coenzyme
Escherichia coli
0.092
(+)-tartrate
thio-NAD+ as coenzyme
Escherichia coli
0.111
(+)-tartrate
NAD+ as coenzyme
Escherichia coli
0.83
(+)-tartrate
Escherichia coli
0.99
D-malate
Escherichia coli
1.63
D-malate
3-acetylpyridine-NAD+ as coenzyme
Escherichia coli
2.62
(+)-tartrate
3-acetylpyridine-NAD+ as coenzyme
Escherichia coli
Organism
Purification (Commentary)
Reaction
meso-tartrate + NAD+ = dihydroxyfumarate + NADH + H+
enzyme catalyzes 3 different chemical reactions from a single active site
Escherichia coli
Substrates and Products (Substrate)
L-(+)-tartrate + NAD+
in presence of Mn2+ and K+ (+)-tartrate is subject to NAD+-dependent oxidation to form oxaloglycolate
349358
Escherichia coli
(3R)-oxaloglycolate + NADH
349358
Escherichia coli
?
malate + NAD+
oxidative decarboxylation in presence of Mn2+ and K+
349358
Escherichia coli
pyruvate + CO2 + NADH
349358
Escherichia coli
?
meso-tartrate + 3-acetylpyridine-NAD+
3-acetylpyridine-NAD+ is a very slow substrate for TDH
349358
Escherichia coli
D-glycerate + CO2 + ?
349358
Escherichia coli
?
meso-tartrate + NAD+
in presence of Mn2+ and K+ reaction requires only catalytic amounts of NAD+
349358
Escherichia coli
D-glycerate + CO2 + NADH
349358
Escherichia coli
?
Synonyms
tartrate dehydrogenase
Escherichia coli
Turnover Number [1/s]
0.417
(+)-tartrate
Escherichia coli
13.3
D-malate
Escherichia coli
Cofactor
3-acetylpyridine adenine dinucleotide
APAD
Escherichia coli
3-pyridinealdehyde adenine dinucleotide
PAAD
Escherichia coli
thio-NAD+
Escherichia coli
Cloned(Commentary) (protein specific)
Escherichia coli cells containing the plasmid pTDH1 which contains the gene encoding TDH under the control of the T7 polymerase promoter
Escherichia coli
Cofactor (protein specific)
3-acetylpyridine adenine dinucleotide
APAD
Escherichia coli
3-pyridinealdehyde adenine dinucleotide
PAAD
Escherichia coli
thio-NAD+
Escherichia coli
Inhibitors (protein specific)
oxalate
time-dependent inhibition
Escherichia coli
KM Value [mM] (protein specific)
0.032
D-malate
thio-NAD+ as coenzyme
Escherichia coli
0.049
D-malate
NAD+ as coenzyme
Escherichia coli
0.092
(+)-tartrate
thio-NAD+ as coenzyme
Escherichia coli
0.111
(+)-tartrate
NAD+ as coenzyme
Escherichia coli
0.83
(+)-tartrate
Escherichia coli
0.99
D-malate
Escherichia coli
1.63
D-malate
3-acetylpyridine-NAD+ as coenzyme
Escherichia coli
2.62
(+)-tartrate
3-acetylpyridine-NAD+ as coenzyme
Escherichia coli
Purification (Commentary) (protein specific)
Substrates and Products (Substrate) (protein specific)
L-(+)-tartrate + NAD+
in presence of Mn2+ and K+ (+)-tartrate is subject to NAD+-dependent oxidation to form oxaloglycolate
349358
Escherichia coli
(3R)-oxaloglycolate + NADH
349358
Escherichia coli
?
malate + NAD+
oxidative decarboxylation in presence of Mn2+ and K+
349358
Escherichia coli
pyruvate + CO2 + NADH
349358
Escherichia coli
?
meso-tartrate + 3-acetylpyridine-NAD+
3-acetylpyridine-NAD+ is a very slow substrate for TDH
349358
Escherichia coli
D-glycerate + CO2 + ?
349358
Escherichia coli
?
meso-tartrate + NAD+
in presence of Mn2+ and K+ reaction requires only catalytic amounts of NAD+
349358
Escherichia coli
D-glycerate + CO2 + NADH
349358
Escherichia coli
?
Turnover Number [1/s] (protein specific)
0.417
(+)-tartrate
Escherichia coli
13.3
D-malate
Escherichia coli
Other publictions for EC 1.3.1.7
349359
Serfozo
Substrate determinants of the ...
Escherichia coli, Pseudomonas putida
Biochemistry
34
7517-7524
1995
-
-
1
-
-
-
-
13
-
-
-
-
-
2
-
-
1
-
-
-
-
-
17
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
1
-
-
-
-
17
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349358
Beecher
Tartrate dehydrogenase-oxalate ...
Escherichia coli
Arch. Biochem. Biophys.
315
255-261
1994
-
-
1
-
-
-
1
8
-
-
-
-
-
1
-
-
1
1
-
-
-
-
4
-
2
-
-
-
2
-
-
-
4
-
-
-
-
-
1
4
-
-
-
-
1
-
8
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
287509
Do Nascimento
The stereospecificity of seque ...
Pseudomonas putida, Pseudomonas putida ATCC 17642
Biochem. J.
149
553-557
1975
-
-
-
-
-
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-
-
-
-
-
-
-
2
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-
1
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2
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1
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2
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-
349356
Kohn
-
L- and mesotartaric acid dehyd ...
Bos taurus, Pseudomonas putida, Pseudomonas sp., Pseudomonas sp. A, Rattus norvegicus
Methods Enzymol.
9
236-240
1966
1
-
-
1
-
-
-
4
2
1
-
3
-
9
-
-
1
-
-
-
1
-
5
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
4
2
1
-
3
-
-
-
1
-
-
1
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-