BRENDA - Enzyme Database
show all sequences of 1.3.1.67

Identification of cis-diols as intermediates in the oxidation of aromatic acids by a strain of Pseudomonas putida that contains a TOL plasmid

Whited, M.G.; McCombie, W.R.; J. Bacteriol. 166, 1028-1039 (1986)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
96000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
carboxylic acid diol + NAD+
Pseudomonas putida
-
catechol + NADH
-
Pseudomonas putida
-
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+
Pseudomonas putida
no activity observed when cis-p-toluene dihydrodiol, cis-naphthalenedihydrodiol, or the methyl ester of cis-p-toluate diol used as a substrate
4-methylcatechol + NADH + H+ + CO2
-
Pseudomonas putida
?
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+
Pseudomonas putida BG1
no activity observed when cis-p-toluene dihydrodiol, cis-naphthalenedihydrodiol, or the methyl ester of cis-p-toluate diol used as a substrate
4-methylcatechol + NADH + H+ + CO2
-
Pseudomonas putida BG1
?
cis-p-toluate diol + NAD+
Pseudomonas putida
metabolism of toluene
4-methylcatechol + NADH + H+ + CO2
-
Pseudomonas putida
?
cis-p-toluate diol + NAD+
Pseudomonas putida BG1
metabolism of toluene
4-methylcatechol + NADH + H+ + CO2
-
Pseudomonas putida BG1
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
harboring TOL plasmid
-
Pseudomonas putida BG1
-
harboring TOL plasmid
-
Purification (Commentary)
Commentary
Organism
French press, DEAE-cellulose column, dialysis, Red A column, ultrafiltration, purified 38fold
Pseudomonas putida
Reaction
Reaction
Commentary
Organism
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD(P)+ = 4-methylcatechol + NAD(P)H + CO2
enzyme utilizes NAD+ but not NAD(P)
Pseudomonas putida
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.067
-
with plasmid pDTG501 and carbon source p-toluate
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-dihydroxycyclohexa-3,5-diene 1-carboxylate + NAD+
-
288487
Pseudomonas putida
catechol + NADH
-
288487
Pseudomonas putida
-
1,2-dihydroxycyclohexa-3,5-diene 1-carboxylate + NAD+
-
288487
Pseudomonas putida BG1
catechol + NADH
-
288487
Pseudomonas putida BG1
-
carboxylic acid diol + NAD+
-
288487
Pseudomonas putida
catechol + NADH
-
288487
Pseudomonas putida
-
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+
-
288487
Pseudomonas putida
4-methylcatechol + NADH + H+ + CO2
-
288487
Pseudomonas putida
?
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+
no activity observed when cis-p-toluene dihydrodiol, cis-naphthalenedihydrodiol, or the methyl ester of cis-p-toluate diol used as a substrate
288487
Pseudomonas putida
4-methylcatechol + NADH + H+ + CO2
-
288487
Pseudomonas putida
?
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+
-
288487
Pseudomonas putida BG1
4-methylcatechol + NADH + H+ + CO2
-
288487
Pseudomonas putida BG1
?
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+
no activity observed when cis-p-toluene dihydrodiol, cis-naphthalenedihydrodiol, or the methyl ester of cis-p-toluate diol used as a substrate
288487
Pseudomonas putida BG1
4-methylcatechol + NADH + H+ + CO2
-
288487
Pseudomonas putida BG1
?
cis-p-toluate diol + NAD+
-
288487
Pseudomonas putida
3-methylcatechol + NADH
-
288487
Pseudomonas putida
?
cis-p-toluate diol + NAD+
-
288487
Pseudomonas putida BG1
3-methylcatechol + NADH
-
288487
Pseudomonas putida BG1
?
cis-p-toluate diol + NAD+
metabolism of toluene
288487
Pseudomonas putida
4-methylcatechol + NADH + H+ + CO2
-
288487
Pseudomonas putida
?
cis-p-toluate diol + NAD+
metabolism of toluene
288487
Pseudomonas putida BG1
4-methylcatechol + NADH + H+ + CO2
-
288487
Pseudomonas putida BG1
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
96000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
carboxylic acid diol + NAD+
Pseudomonas putida
-
catechol + NADH
-
Pseudomonas putida
-
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+
Pseudomonas putida
no activity observed when cis-p-toluene dihydrodiol, cis-naphthalenedihydrodiol, or the methyl ester of cis-p-toluate diol used as a substrate
4-methylcatechol + NADH + H+ + CO2
-
Pseudomonas putida
?
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+
Pseudomonas putida BG1
no activity observed when cis-p-toluene dihydrodiol, cis-naphthalenedihydrodiol, or the methyl ester of cis-p-toluate diol used as a substrate
4-methylcatechol + NADH + H+ + CO2
-
Pseudomonas putida BG1
?
cis-p-toluate diol + NAD+
Pseudomonas putida
metabolism of toluene
4-methylcatechol + NADH + H+ + CO2
-
Pseudomonas putida
?
cis-p-toluate diol + NAD+
Pseudomonas putida BG1
metabolism of toluene
4-methylcatechol + NADH + H+ + CO2
-
Pseudomonas putida BG1
?
Purification (Commentary) (protein specific)
Commentary
Organism
French press, DEAE-cellulose column, dialysis, Red A column, ultrafiltration, purified 38fold
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.067
-
with plasmid pDTG501 and carbon source p-toluate
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-dihydroxycyclohexa-3,5-diene 1-carboxylate + NAD+
-
288487
Pseudomonas putida
catechol + NADH
-
288487
Pseudomonas putida
-
1,2-dihydroxycyclohexa-3,5-diene 1-carboxylate + NAD+
-
288487
Pseudomonas putida BG1
catechol + NADH
-
288487
Pseudomonas putida BG1
-
carboxylic acid diol + NAD+
-
288487
Pseudomonas putida
catechol + NADH
-
288487
Pseudomonas putida
-
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+
-
288487
Pseudomonas putida
4-methylcatechol + NADH + H+ + CO2
-
288487
Pseudomonas putida
?
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+
no activity observed when cis-p-toluene dihydrodiol, cis-naphthalenedihydrodiol, or the methyl ester of cis-p-toluate diol used as a substrate
288487
Pseudomonas putida
4-methylcatechol + NADH + H+ + CO2
-
288487
Pseudomonas putida
?
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+
-
288487
Pseudomonas putida BG1
4-methylcatechol + NADH + H+ + CO2
-
288487
Pseudomonas putida BG1
?
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+
no activity observed when cis-p-toluene dihydrodiol, cis-naphthalenedihydrodiol, or the methyl ester of cis-p-toluate diol used as a substrate
288487
Pseudomonas putida BG1
4-methylcatechol + NADH + H+ + CO2
-
288487
Pseudomonas putida BG1
?
cis-p-toluate diol + NAD+
-
288487
Pseudomonas putida
3-methylcatechol + NADH
-
288487
Pseudomonas putida
?
cis-p-toluate diol + NAD+
-
288487
Pseudomonas putida BG1
3-methylcatechol + NADH
-
288487
Pseudomonas putida BG1
?
cis-p-toluate diol + NAD+
metabolism of toluene
288487
Pseudomonas putida
4-methylcatechol + NADH + H+ + CO2
-
288487
Pseudomonas putida
?
cis-p-toluate diol + NAD+
metabolism of toluene
288487
Pseudomonas putida BG1
4-methylcatechol + NADH + H+ + CO2
-
288487
Pseudomonas putida BG1
?
Other publictions for EC 1.3.1.67
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
288487
Whited
Identification of cis-diols as ...
Pseudomonas putida, Pseudomonas putida BG1
J. Bacteriol.
166
1028-1039
1986
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1
5
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2
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1
1
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1
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11
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1
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1
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1
5
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1
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1
-
11
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