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Literature summary for 1.3.1.6 extracted from
- Fumarate reductase activity of Streptococcus faecalis (1967), J. Bacteriol., 93, 1770-1776.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| alanine | - |
Enterococcus faecalis |  |
| glycine | - |
Enterococcus faecalis | |
| histidine | - |
Enterococcus faecalis | |
General Stability
| General Stability | Organism |
|---|---|
| stabilized against air oxidation with 10 mM thioglycollate | Enterococcus faecalis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| citraconate | slight inhibition | Enterococcus faecalis | |
| iodoacetate | slight inhibition | Enterococcus faecalis | |
| malonate | slight inhibition | Enterococcus faecalis | |
| p-chloromercuribenzoate | slight inhibition | Enterococcus faecalis | |
| succinate | slight inhibition | Enterococcus faecalis | |
| trans-, trans muconate | slight inhibition | Enterococcus faecalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
FMNH2 | - |
Enterococcus faecalis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fumarate + NADH + H+ | Enterococcus faecalis | - |
succinate + NAD+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus faecalis | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| sensitive to air oxidation, loss of activity | Enterococcus faecalis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Enterococcus faecalis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.23 | - |
in presence of 10 mM thioglycolate | Enterococcus faecalis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| stable in lyophylized whole cells | Enterococcus faecalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fumarate + FMNH2 | - |
Enterococcus faecalis | succinate + FMN | - |
r | |
| fumarate + NADH + H+ | - |
Enterococcus faecalis | succinate + NAD+ | - |
? | |
| fumarate + NADH + H+ | FMNH2 serves as cofactor | Enterococcus faecalis | succinate + NAD+ | - |
ir | |
| maleate + NADH | - |
Enterococcus faecalis | succinate + NAD+ | - |
ir | |
| mesaconate + NADH | - |
Enterococcus faecalis | methylsuccinate + NAD+ | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Enterococcus faecalis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | - |
Enterococcus faecalis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMNH2 | - |
Enterococcus faecalis | |
| NADH | - |
Enterococcus faecalis | |
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Release 2023.1 (January 2023)
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