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Literature summary for 1.3.1.54 extracted from

  • Roessner, C.A.; Spencer, J.B.; Ozaki, S.; Min, C.; Atshaves, B.P.; Nayar, P.; Anousis, N.; Stolowich, N.J.; Holderman, M.T.; Scott, A.I.
    Overexpression in Escherichia coli of 12 vitamin B12 biosynthetic enzymes (1995), Protein Expr. Purif., 6, 155-163.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
functional overexpression of gene cobK together with the other 11 of the first 12 enzymes involved in biosynthesis of cobalamin, vitamin B12, from aminolevulinic acid, in Escherichia coli strains TB1 and BL21(DE3) Pseudomonas denitrificans (nom. rej.)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
x * 28095, sequence calculation, x * 26000, recombinant enzyme, SDS-PAGE Pseudomonas denitrificans (nom. rej.)
28095
-
x * 28095, sequence calculation, x * 26000, recombinant enzyme, SDS-PAGE Pseudomonas denitrificans (nom. rej.)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
precorrin-6A + NADPH Pseudomonas denitrificans (nom. rej.) enzyme catalyzes step 10 in the biosynthesis of cobalamine, vitamin B12, pathway overview precorrin-6B + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas denitrificans (nom. rej.)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble enzyme from Escherichia coli Pseudomonas denitrificans (nom. rej.)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
precorrin-6A + NADPH
-
Pseudomonas denitrificans (nom. rej.) precorrin-6B + NADP+
-
?
precorrin-6A + NADPH enzyme catalyzes step 10 in the biosynthesis of cobalamine, vitamin B12, pathway overview Pseudomonas denitrificans (nom. rej.) precorrin-6B + NADP+
-
?

Subunits

Subunits Comment Organism
? x * 28095, sequence calculation, x * 26000, recombinant enzyme, SDS-PAGE Pseudomonas denitrificans (nom. rej.)

Synonyms

Synonyms Comment Organism
precorrin-6 reductase
-
Pseudomonas denitrificans (nom. rej.)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pseudomonas denitrificans (nom. rej.)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Pseudomonas denitrificans (nom. rej.)

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Pseudomonas denitrificans (nom. rej.)