Cloned (Comment) | Organism |
---|---|
functional overexpression of gene cobK together with the other 11 of the first 12 enzymes involved in biosynthesis of cobalamin, vitamin B12, from aminolevulinic acid, in Escherichia coli strains TB1 and BL21(DE3) | Pseudomonas denitrificans (nom. rej.) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
x * 28095, sequence calculation, x * 26000, recombinant enzyme, SDS-PAGE | Pseudomonas denitrificans (nom. rej.) |
28095 | - |
x * 28095, sequence calculation, x * 26000, recombinant enzyme, SDS-PAGE | Pseudomonas denitrificans (nom. rej.) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
precorrin-6A + NADPH | Pseudomonas denitrificans (nom. rej.) | enzyme catalyzes step 10 in the biosynthesis of cobalamine, vitamin B12, pathway overview | precorrin-6B + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas denitrificans (nom. rej.) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant soluble enzyme from Escherichia coli | Pseudomonas denitrificans (nom. rej.) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
precorrin-6A + NADPH | - |
Pseudomonas denitrificans (nom. rej.) | precorrin-6B + NADP+ | - |
? | |
precorrin-6A + NADPH | enzyme catalyzes step 10 in the biosynthesis of cobalamine, vitamin B12, pathway overview | Pseudomonas denitrificans (nom. rej.) | precorrin-6B + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28095, sequence calculation, x * 26000, recombinant enzyme, SDS-PAGE | Pseudomonas denitrificans (nom. rej.) |
Synonyms | Comment | Organism |
---|---|---|
precorrin-6 reductase | - |
Pseudomonas denitrificans (nom. rej.) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas denitrificans (nom. rej.) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudomonas denitrificans (nom. rej.) |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Pseudomonas denitrificans (nom. rej.) |