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Literature summary for 1.3.1.48 extracted from

  • Zhao, Y.; Weng, C.C.; Tong, M.; Wei, J.; Tai, H.H.
    Restoration of leukotriene B(4)-12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase (LTBDH/PGR) expression inhibits lung cancer growth in vitro and in vivo (2010), Lung Cancer, 68, 161-169.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.48

Cloned(Commentary)

Cloned (Comment) Organism
effects of LTBDH/PGR expression in LTBDH/PGR-negative and -positive lung cancer cells by infection with adenovirus carrying the LTBDH/PGR gene and by stable transfection with an inducible LTBDH/PGR expression vector Sus scrofa
expressed in H1299, A549, H157 and AD-293 cells applying an adenoviral shuttle vector Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Sus scrofa bifunctional enzyme capable of inactivating leukotriene B4 and 15-oxo-prostaglandins, enzyme plays a role in growth suppressive functions in lung cancer ?
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 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa Q29073
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-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 after restoration of LTBDH/PGR expression in LTBDH/PGR-negative (H1299) or -low (A549) lung cancer cell lines, the restored enzyme induces apoptosis and growth inhibition in vitro, restoration of LTBDH/PGR expression is effective in preventing lung cancer growth in vitro and in vivo Sus scrofa
additional information
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 cell cycle alterations induced by LTBDH/PGR over-expression are assessed with Ad-GFP or Ad-PGR after infection at 100 MOI for 72 h, after infection with Ad-PGR, the apoptosis population in A549 cells and H1299 cells is 8.59% and 9.57%, whereas apoptosis population is not detected significantly in H157 infected cells Sus scrofa
additional information
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 effects of induced expression of LTBDH/PGR in H1299 cells, LTBDH/PGR expression increases in a dose and time dependent manner after tetracycline treatment, marked expression is observed at day 4 and significantly affects cell proliferation beginning day 5 causing reduction in cell number and suggesting that LTBDH/PGR expression inhibit growth of H1299 cells Sus scrofa
additional information
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 the expression of apoptosis-related proteins BAX, Bcl-2 and CD44 in H1299 and H157 cells is not changed by LTBDH/PGR over-expression except for BAX which is decreased in H157 cells, endogenous p53 and p21 are not expressed in both H1299 and H157 cells Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information bifunctional enzyme capable of inactivating leukotriene B4 and 15-oxo-prostaglandins, enzyme plays a role in growth suppressive functions in lung cancer Sus scrofa ?
-
 ?

Synonyms

Synonyms Comment Organism
Ad-PGR
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 Sus scrofa
leukotriene B4-12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase
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 Sus scrofa
LTBDH/PGR
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 Sus scrofa
PGR
-
 Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NAD(P)H
-
 Sus scrofa