Literature summary for 1.3.1.45 extracted from

Castro, L.; Crespo, J.F.; Rodriguez, J.; Rodriguez, R.; Villalba, M.
Immunoproteomic tools are used to identify masked allergens Ole e 12, an allergenic isoflavone reductase from olive (Olea europaea) pollen (2015), Biochim. Biophys. Acta, 1854, 1871-1880 .

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Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence dteremination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) by dialysis, nickel affinity chromatography, and proteolytic digestion of fusion protein with Factor Xa protease	Olea europaea

Organism

Organism	UniProt	Comment	Textmining
Olea europaea	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from cell free extract of Escherichia coli strain BL21(DE3)	Olea europaea

Subunits

Subunits	Comment	Organism
?	x * 37000, SDS-PAGE, x * 34285, recombinant His-tagged enzyme, mass spectrometry	Olea europaea

Synonyms

Synonyms	Comment	Organism
Ole e 12	-	Olea europaea

pI Value

Organism	Comment	pI Value Maximum	pI Value
Olea europaea	sequence calculation	-	5.7

General Information

General Information	Comment	Organism
evolution	the allergen, named Ole e 12, is a polymorphic isoflavone reductase-like protein of 308 amino acids showing 80% and 74% identity with birch and pear allergens, Bet v 6 and Pyr c 5, respectively. Analysis of the presence of Ole e 12 homologues in different pollens from other species, overview	Olea europaea
physiological function	the 37-kDa enzyme protein seems to be associated to the IgE-binding profile of a group of patients suffering allergy to peach and olive pollen. The allergen, named Ole e 12, is a polymorphic isoflavone reductase-like protein of 308 amino acids showing 80% and 74% identity with birch and pear allergens, Bet v 6 and Pyr c 5, respectively. Ole e 12 is a minor olive pollen allergen, which gains relevance in patients allergic to peach with olive pollinosis	Olea europaea

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Release 2023.1 (January 2023)