Cloned (Comment) | Organism |
---|---|
into Escherichia coli expression vector pET28a | Medicago sativa |
Crystallization (Comment) | Organism |
---|---|
at 1.6 A resolution by hanging-drop vapor-diffusion method | Medicago sativa |
Protein Variants | Comment | Organism |
---|---|---|
D285G | decreased turnover rate | Medicago sativa |
DELTA39-47 | nine of the amino acid residues around position 40 deleted in the current construct D39-47 IFR to facilitate crystallization, affects enzyme activity only slightly | Medicago sativa |
G14F | no detectable activity | Medicago sativa |
H164A | decreased turnover rate | Medicago sativa |
K144A | complete loss of enzyme activity | Medicago sativa |
R97G | decreased turnover rate | Medicago sativa |
Y169A | decreased turnover rate | Medicago sativa |
Y272A | decreased turnover rate | Medicago sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0142 | - |
2'-hydroxyformononetin | wild-type | Medicago sativa | |
0.0157 | - |
2'-hydroxyformononetin | mutant Y272A | Medicago sativa | |
0.0158 | - |
2'-hydroxyformononetin | mutant R97G | Medicago sativa | |
0.0162 | - |
2'-hydroxyformononetin | mutant DELTA39-47 IFR | Medicago sativa | |
0.0166 | - |
2'-hydroxyformononetin | mutant Y169A | Medicago sativa | |
0.018 | - |
2'-hydroxyformononetin | mutant D285G | Medicago sativa | |
0.0182 | - |
2'-hydroxyformononetin | mutant H164A | Medicago sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago sativa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA affinity chromatography and gel filtration | Medicago sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-hydroxyformononetin + NADPH | - |
Medicago sativa | vestitone + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | gel filtration | Medicago sativa |
Synonyms | Comment | Organism |
---|---|---|
IFR | - |
Medicago sativa |
isoflavone reductase | - |
Medicago sativa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00383 | - |
2'-hydroxyformononetin | mutant Y272A | Medicago sativa | |
0.004167 | - |
2'-hydroxyformononetin | mutant D285G | Medicago sativa | |
0.0067 | - |
2'-hydroxyformononetin | mutant Y169A | Medicago sativa | |
0.0097 | - |
2'-hydroxyformononetin | mutant R97G | Medicago sativa | |
0.00983 | - |
2'-hydroxyformononetin | mutant H164A | Medicago sativa | |
0.063 | - |
2'-hydroxyformononetin | mutant DELTA39-47 IFR | Medicago sativa | |
0.215 | - |
2'-hydroxyformononetin | wild-type | Medicago sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Medicago sativa |