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Literature summary for 1.3.1.45 extracted from
- Isolation of NADPH:isoflavone oxidoreductase, a new enzyme of pterocarpan phtoalexin biosynthesi in cell suspension cultures of Cicer arietinum (1987), FEBS Lett., 213, 324-328.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
2'-hydroxyformononetin | - |
Cicer arietinum |  |
| 0.035 | - |
NADPH | - |
Cicer arietinum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Cicer arietinum | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-hydroxyformononetin + NADPH | Cicer arietinum | involved in medicarpin and maackiain biosynthesis | vestitone + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cicer arietinum | - |
chickpea | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| complete inactivation without dithioerythritol in 6 h at 4°C | Cicer arietinum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Cicer arietinum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Cicer arietinum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.012 | - |
- |
Cicer arietinum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 20 mM potassium phosphate buffer, pH 7.5, 1 mM dithioerythritol, 5% glycerol | Cicer arietinum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2',7-dihydroxy-3',4'-methylenedioxyisoflavone + NADPH | - |
Cicer arietinum | 2',7-hydroxy-dihydro-3',4'-methylenedioxyisoflavone + NADP+ | - |
? | |
| 2'-hydroxyformononetin + NADPH | no reaction with isoflavones lacking 2'-hydroxy-group | Cicer arietinum | (3R)-vestitone + NADP+ | - |
? | |
| 2'-hydroxyformononetin + NADPH | involved in medicarpin and maackiain biosynthesis | Cicer arietinum | vestitone + NADP+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 40 | - |
Cicer arietinum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Cicer arietinum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | 25-40% of activity with NADPH | Cicer arietinum | |
| NADPH | - |
Cicer arietinum | |
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Release 2023.1 (January 2023)
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