Cloned (Comment) | Organism |
---|---|
wild-type and deletion mutant BatG expression in Pseudomonas fluorescens strain BCCM_ID9359, Escherichia coli, and Staphylococcus aureus, confers resistance against kalimantacin/batumin | Pseudomonas fluorescens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a specific in vivo gene inactivation by in-frame deletion of BatG | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas fluorescens BCCM_ID9359 | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
BatG | - |
Pseudomonas fluorescens |
More | BatG as a trans-2-enoyl-ACP reductase isozyme, an isozyme of FabI, it contains the conserved NADH-binding motif GxxxGxG and catalytic triad YYK | Pseudomonas fluorescens |
trans-2-enoyl-ACP reductase | - |
Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas fluorescens | |
NADH | - |
Pseudomonas fluorescens | |
NADP+ | - |
Pseudomonas fluorescens | |
NADPH | - |
Pseudomonas fluorescens |
General Information | Comment | Organism |
---|---|---|
additional information | BatG is an isoform of FabI, conferring full resistance to target bacteria against kalimantacin/batumin and/or triclosan | Pseudomonas fluorescens |
physiological function | BatG encodes a functional FabI isozyme which confers full resistance to kalimantacin/batumin and complements FabI. BatG is, similarity to trans-2-enoyl-ACP reductases, involved in the formation of a saturated acyl-ACP by an NAD(P)H-dependent reduction of the trans-2-enoyl-ACP double bond, which is essential for the final step of the elongation cycle of fatty acid biosynthesis. BatG knockout does not influence the kalimantacin/batumin, kal/bat, biosynthesis structurally. Kalimantacin/batumin biosynthesis is BatG-independent, despite BatG localization in the operon among kal/bat tailoring enzymes, overview | Pseudomonas fluorescens |