BRENDA - Enzyme Database show
show all sequences of 1.3.1.43

Arogenate (pretyrosine) is an obligatory intermediate of L-tyrosine biosynthesis: confirmation in a microbial mutant

Fazel, A.M.; Bowen, J.R.; Jensen, R.A.; Proc. Natl. Acad. Sci. USA 77, 1270-1273 (1980)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
additional information
spontaneous mutant tyrB2 without any activity
Brevibacterium flavum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-arogenate + NAD+
Brevibacterium flavum
i.e. 3-(1-carboxy-4-hydroxycyclohexa-2,5-dien-1-yl)-L-alanine, final biosynthetic step to tyrosine
L-tyrosine + NADH + CO2
-
Brevibacterium flavum
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Brevibacterium flavum
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.0134
-
-
Brevibacterium flavum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-arogenate + NAD(P)+
-
390798
Brevibacterium flavum
L-tyrosine + NAD(P)H + CO2
-
390798
Brevibacterium flavum
?
L-arogenate + NAD+
i.e. 3-(1-carboxy-4-hydroxycyclohexa-2,5-dien-1-yl)-L-alanine, final biosynthetic step to tyrosine
390798
Brevibacterium flavum
L-tyrosine + NADH + CO2
-
390798
Brevibacterium flavum
?
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
spontaneous mutant tyrB2 without any activity
Brevibacterium flavum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-arogenate + NAD+
Brevibacterium flavum
i.e. 3-(1-carboxy-4-hydroxycyclohexa-2,5-dien-1-yl)-L-alanine, final biosynthetic step to tyrosine
L-tyrosine + NADH + CO2
-
Brevibacterium flavum
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.0134
-
-
Brevibacterium flavum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-arogenate + NAD(P)+
-
390798
Brevibacterium flavum
L-tyrosine + NAD(P)H + CO2
-
390798
Brevibacterium flavum
?
L-arogenate + NAD+
i.e. 3-(1-carboxy-4-hydroxycyclohexa-2,5-dien-1-yl)-L-alanine, final biosynthetic step to tyrosine
390798
Brevibacterium flavum
L-tyrosine + NADH + CO2
-
390798
Brevibacterium flavum
?
Other publictions for EC 1.3.1.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725624
Holding
Identification and characteriz ...
Zea mays
J. Exp. Bot.
61
3663-3673
2010
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1
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1
1
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390802
Xie
Cyclohexadienyl dehydrogenase ...
Pseudomonas stutzeri, Pseudomonas stutzeri JM300
Comp. Biochem. Physiol. C
125
65-83
2000
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-
1
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13
3
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2
2
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1
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390804
Zhao
An allosterically insensitive ...
Zymomonas mobilis
Eur. J. Biochem.
212
157-165
1993
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1
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3
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9
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5
1
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390803
Xia
A single cyclohexadienyl dehyd ...
Pseudomonas aeruginosa
J. Biol. Chem.
265
20033-20036
1990
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3
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1
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2
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1
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1
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1
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390785
Hund
Purification and properties of ...
Actinoplanes missouriensis
Z. Naturforsch. C
44
797-801
1989
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1
2
2
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2
1
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2
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1
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1
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1
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1
1
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1
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2
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2
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2
1
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1
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1
1
2
1
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1
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1
1
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390512
Berry
Enzymic arrangement and allost ...
Neisseria gonorrhoeae
Arch. Microbiol.
149
87-94
1987
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390519
Hund
-
Phenylalanine and tyrosine bio ...
Actinomadura citrea, Actinoplanes auranticolor, Actinoplanes campanulatus, Actinoplanes missouriensis, Actinoplanes philippinensis, Dactylosporangium thailandense, Micromonospora echinospora, Pilimelia terevasa, Planobispora longispora, Planomonospora venezuelensis, Spirillospora albida, Streptomyces humiferus, Streptosporangium roseum
Z. Naturforsch. C
42
387-393
1987
8
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12
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13
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13
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26
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12
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8
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12
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12
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13
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13
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13
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26
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390548
Ahmad
-
Evolution of the biochemical p ...
Pseudomonas flexibilis
Arch. Microbiol.
147
8-12
1987
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1
2
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1
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1
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390782
Lingens
-
Arogenate dehydrogenase from P ...
Phenylobacterium immobile
Methods Enzymol.
142
513-518
1987
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1
2
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2
1
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1
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1
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1
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1
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390783
Keller
Arogenate dehydrogenase from S ...
Streptomyces phaeochromogenes
Biol. Chem. Hoppe-Seyler
366
1063-1066
1985
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2
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390784
Mayer
Purification of arogenate dehy ...
Phenylobacterium immobile
FEBS Lett.
179
208-212
1985
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1
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390791
Byng
-
Enzymology of L-tyrosine biosy ...
Zea mays
Phytochemistry
20
1289-1292
1981
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390797
Hall
Enzymological basis for growth ...
Synechocystis sp. 29108, Synechocystis sp.
J. Bacteriol.
144
1034-1042
1980
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390798
Fazel
Arogenate (pretyrosine) is an ...
Brevibacterium flavum
Proc. Natl. Acad. Sci. USA
77
1270-1273
1980
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