BRENDA - Enzyme Database show
show all sequences of 1.3.1.43

Purification of arogenate dehydrogenase from Phenylobacterium immobile

Mayer, E.; Waldner-Sander, S.; Keller, B.; Keller, E.; Lingens, F.; FEBS Lett. 179, 208-212 (1985)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
prephenate
competitive inhibition
Phenylobacterium immobile
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
NAD+
-
Phenylobacterium immobile
0.09
-
L-arogenate
-
Phenylobacterium immobile
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38400
-
2 * 38400, SDS-PAGE
Phenylobacterium immobile
69000
-
gel electrophoresis after cross-linking with dimethylsuberimidate
Phenylobacterium immobile
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-arogenate + NAD+
Phenylobacterium immobile
i.e. 3-(1-carboxy-4-hydroxycyclohexa-2,5-dien-1-yl)-L-alanine, final biosynthetic step to tyrosine
L-tyrosine + NADH + CO2
-
Phenylobacterium immobile
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Phenylobacterium immobile
-
-
-
Purification (Commentary)
Commentary
Organism
-
Phenylobacterium immobile
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Phenylobacterium immobile
95.5
-
-
Phenylobacterium immobile
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-arogenate + NAD(P)+
i.e. 3-(1-carboxy-4-hydroxycyclohexa-2,5-dien-1-yl)-L-alanine
390784
Phenylobacterium immobile
L-tyrosine + NAD(P)H + CO2
-
390784
Phenylobacterium immobile
?
L-arogenate + NAD+
i.e. 3-(1-carboxy-4-hydroxycyclohexa-2,5-dien-1-yl)-L-alanine, final biosynthetic step to tyrosine
390784
Phenylobacterium immobile
L-tyrosine + NADH + CO2
-
390784
Phenylobacterium immobile
?
additional information
highly specific for arogenate
390784
Phenylobacterium immobile
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 38400, SDS-PAGE
Phenylobacterium immobile
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Phenylobacterium immobile
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Phenylobacterium immobile
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Phenylobacterium immobile
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
prephenate
competitive inhibition
Phenylobacterium immobile
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
NAD+
-
Phenylobacterium immobile
0.09
-
L-arogenate
-
Phenylobacterium immobile
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38400
-
2 * 38400, SDS-PAGE
Phenylobacterium immobile
69000
-
gel electrophoresis after cross-linking with dimethylsuberimidate
Phenylobacterium immobile
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-arogenate + NAD+
Phenylobacterium immobile
i.e. 3-(1-carboxy-4-hydroxycyclohexa-2,5-dien-1-yl)-L-alanine, final biosynthetic step to tyrosine
L-tyrosine + NADH + CO2
-
Phenylobacterium immobile
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Phenylobacterium immobile
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Phenylobacterium immobile
95.5
-
-
Phenylobacterium immobile
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-arogenate + NAD(P)+
i.e. 3-(1-carboxy-4-hydroxycyclohexa-2,5-dien-1-yl)-L-alanine
390784
Phenylobacterium immobile
L-tyrosine + NAD(P)H + CO2
-
390784
Phenylobacterium immobile
?
L-arogenate + NAD+
i.e. 3-(1-carboxy-4-hydroxycyclohexa-2,5-dien-1-yl)-L-alanine, final biosynthetic step to tyrosine
390784
Phenylobacterium immobile
L-tyrosine + NADH + CO2
-
390784
Phenylobacterium immobile
?
additional information
highly specific for arogenate
390784
Phenylobacterium immobile
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 38400, SDS-PAGE
Phenylobacterium immobile
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Phenylobacterium immobile
Other publictions for EC 1.3.1.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725624
Holding
Identification and characteriz ...
Zea mays
J. Exp. Bot.
61
3663-3673
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
390802
Xie
Cyclohexadienyl dehydrogenase ...
Pseudomonas stutzeri, Pseudomonas stutzeri JM300
Comp. Biochem. Physiol. C
125
65-83
2000
-
-
1
-
-
-
13
3
-
-
2
2
-
5
-
-
1
1
-
-
-
-
8
1
1
-
-
-
1
1
-
1
2
-
-
-
-
1
1
-
-
-
-
13
2
3
-
-
2
2
-
-
-
1
-
-
-
-
8
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
390804
Zhao
An allosterically insensitive ...
Zymomonas mobilis
Eur. J. Biochem.
212
157-165
1993
-
-
1
-
-
-
1
3
-
-
1
2
-
9
-
-
1
1
-
-
2
-
5
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
3
-
-
1
2
-
-
-
1
-
-
2
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390803
Xia
A single cyclohexadienyl dehyd ...
Pseudomonas aeruginosa
J. Biol. Chem.
265
20033-20036
1990
-
-
-
-
-
-
1
3
-
-
2
1
-
2
-
-
1
-
-
-
-
-
4
1
-
-
-
-
-
-
-
1
2
-
-
-
-
-
1
-
-
-
-
1
2
3
-
-
2
1
-
-
-
1
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390785
Hund
Purification and properties of ...
Actinoplanes missouriensis
Z. Naturforsch. C
44
797-801
1989
-
-
-
-
-
1
2
2
-
-
2
1
-
2
-
-
1
-
-
-
1
1
2
1
-
-
1
-
1
1
-
1
-
-
-
-
-
-
1
-
-
1
-
2
-
2
-
-
2
1
-
-
-
1
-
-
1
1
2
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
390512
Berry
Enzymic arrangement and allost ...
Neisseria gonorrhoeae
Arch. Microbiol.
149
87-94
1987
-
-
-
-
-
-
4
-
-
-
-
1
-
2
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390519
Hund
-
Phenylalanine and tyrosine bio ...
Actinomadura citrea, Actinoplanes auranticolor, Actinoplanes campanulatus, Actinoplanes missouriensis, Actinoplanes philippinensis, Dactylosporangium thailandense, Micromonospora echinospora, Pilimelia terevasa, Planobispora longispora, Planomonospora venezuelensis, Spirillospora albida, Streptomyces humiferus, Streptosporangium roseum
Z. Naturforsch. C
42
387-393
1987
8
-
-
-
-
-
12
-
-
-
-
13
-
13
-
-
13
-
-
-
13
-
26
-
-
-
-
-
-
-
-
12
-
-
-
8
-
-
12
-
-
-
-
12
-
-
-
-
-
13
-
-
-
13
-
-
13
-
26
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390548
Ahmad
-
Evolution of the biochemical p ...
Pseudomonas flexibilis
Arch. Microbiol.
147
8-12
1987
-
-
-
-
-
-
1
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
1
1
2
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
390782
Lingens
-
Arogenate dehydrogenase from P ...
Phenylobacterium immobile
Methods Enzymol.
142
513-518
1987
-
-
-
-
-
-
1
2
-
-
2
1
-
1
-
-
1
-
-
-
1
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
2
1
-
-
-
1
-
-
1
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
390783
Keller
Arogenate dehydrogenase from S ...
Streptomyces phaeochromogenes
Biol. Chem. Hoppe-Seyler
366
1063-1066
1985
-
-
-
-
-
-
-
2
-
-
2
1
-
2
-
-
1
-
-
-
2
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
2
1
-
-
-
1
-
-
2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390784
Mayer
Purification of arogenate dehy ...
Phenylobacterium immobile
FEBS Lett.
179
208-212
1985
-
-
-
-
-
-
1
2
-
-
2
1
-
2
-
-
1
-
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2
-
3
1
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1
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1
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1
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1
-
2
-
-
2
1
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1
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2
-
3
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
390791
Byng
-
Enzymology of L-tyrosine biosy ...
Zea mays
Phytochemistry
20
1289-1292
1981
-
-
-
-
-
-
1
2
-
-
-
1
-
1
-
-
1
-
-
1
-
-
2
-
-
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-
1
-
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-
1
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1
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2
-
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-
1
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1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390797
Hall
Enzymological basis for growth ...
Synechocystis sp. 29108, Synechocystis sp.
J. Bacteriol.
144
1034-1042
1980
-
-
-
-
1
-
1
-
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2
-
2
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1
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4
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1
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1
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1
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4
-
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390798
Fazel
Arogenate (pretyrosine) is an ...
Brevibacterium flavum
Proc. Natl. Acad. Sci. USA
77
1270-1273
1980
-
-
-
-
1
-
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1
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3
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1
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1
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1
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1
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