Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.42 extracted from

  • Ishiga, Y.; Funato, A.; Tachiki, T.; Toyoda, K.; Shiraishi, T.; Yamada, T.; Ichinose, Y.
    Expression of the 12-oxophytodienoic acid 10,11-reductase gene in the compatible interaction between pea and fungal pathogen (2002), Plant Cell Physiol., 43, 1210-1220.
    View publication on PubMed
Search for more literature for 1.3.1.42

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme expression is inducible by regulatory suppressor glycopeptides from the fungus Mycosphaerella pinodes, which block the pea defense response induced by elicitors Pisum sativum

Cloned(Commentary)

Cloned (Comment) Organism
cDNA library construction, protein S64, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) as His-tagged protein Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
 (15Z)-12-oxophyto-10,15-dienoate pH 7.5, 25°C, recombinant enzyme Pisum sativum
0.055
-
 2-cyclohexen-1-one pH 7.5, 25°C, recombinant enzyme Pisum sativum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pisum sativum suppressor-inducible enzyme activity leads to jasmonate biosynthesis or related compound, which might contribute to compatibility by specific activation of the octadecanoid pathway and inhibition of the phenylpropanoid biosynthetic pathway ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pisum sativum Q9AVK9 cv. Midoriusui
-

Source Tissue

Source Tissue Comment Organism Textmining
epicotyl
-
 Pisum sativum
-
leaf
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(15Z)-12-oxophyto-10,15-dienoate + NADPH
-
 Pisum sativum 8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
 ?
(15Z)-12-oxophyto-10,15-dienoate + NADPH + H+
-
 Pisum sativum 8-[(1R,2R)-3-oxo-2-{(Z)-pent-2-enyl}cyclopentyl]octanoate + NADP+
-
 r
2-cyclohexen-1-one
-
 Pisum sativum ?
-
 r
2-cyclohexen-1-one + NADPH best substrate Pisum sativum ? + NADP+
-
 ?
additional information suppressor-inducible enzyme activity leads to jasmonate biosynthesis or related compound, which might contribute to compatibility by specific activation of the octadecanoid pathway and inhibition of the phenylpropanoid biosynthetic pathway Pisum sativum ?
-
 ?

Synonyms

Synonyms Comment Organism
S64
-
 Pisum sativum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pisum sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Pisum sativum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Pisum sativum