Literature summary for 1.3.1.39 extracted from

Hevener, K.E.; Mehboob, S.; Boci, T.; Truong, K.; Santarsiero, B.D.; Johnson, M.E.
Expression, purification and characterization of enoyl-ACP reductase II, FabK, from Porphyromonas gingivalis (2012), Protein Expr. Purif., 85, 100-108.

Search for more literature for 1.3.1.39

Application

Application	Comment	Organism
analysis	sensitive assay method measuring the fluorescence decrease of NADPH as it is converted to NADP+ during the reaction, optimized for high-throughput screening	Porphyromonas gingivalis

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Porphyromonas gingivalis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.017	-	NADPH	pH 7.5, 25°C	Porphyromonas gingivalis
0.0478	-	crotonyl-CoA	pH 7.5, 25°C	Porphyromonas gingivalis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33090	-	x * 33090, calculated	Porphyromonas gingivalis

Organism

Organism	UniProt	Comment	Textmining
Porphyromonas gingivalis	Q7MAW0	-	-
Porphyromonas gingivalis W83	Q7MAW0	-	-

Storage Stability

Storage Stability	Organism
-20°C, 5 mg/ml concentration, 30% glycerol stock, stable for at least 1 month	Porphyromonas gingivalis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
crotonyl-CoA + NADPH + H+	-	Porphyromonas gingivalis	butyryl-CoA + NADP+	-	?
crotonyl-CoA + NADPH + H+	-	Porphyromonas gingivalis W83	butyryl-CoA + NADP+	-	?

Subunits

Subunits	Comment	Organism
?	x * 33090, calculated	Porphyromonas gingivalis

Synonyms

Synonyms	Comment	Organism
FabK	-	Porphyromonas gingivalis
PG1416	locus name	Porphyromonas gingivalis

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Porphyromonas gingivalis

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Release 2023.1 (January 2023)