BRENDA - Enzyme Database
show all sequences of 1.3.1.38

Evidence from Raman spectroscopy that InhA, the mycobacterial enoyl reductase, modulates the conformation of the NADH cofactor to promote catalysis

Bell, A.F.; Stratton, C.F.; Zhang, X.; Novichenok, P.; Jaye, A.A.; Nair, P.A.; Parikh, S.; Rawat, R.; Tonge, P.J.; J. Am. Chem. Soc. 129, 6425-6431 (2007) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3)pLysS cells
Mycobacterium tuberculosis
Engineering
Protein Variants
Commentary
Organism
F149A
the mutant is not able to optimally position the cofactor for hydride transfer, which correlates with the 30fold decrease in kcat
Mycobacterium tuberculosis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00433
-
2-dodecenoyl-CoA
mutant enzyme F149A, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)
Mycobacterium tuberculosis
0.0054
-
2-dodecenoyl-CoA
wild type enzyme, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)
Mycobacterium tuberculosis
0.0152
-
NADH
mutant enzyme F149A, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)
Mycobacterium tuberculosis
0.0352
-
NADH
wild type enzyme, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)
Mycobacterium tuberculosis
Organism
Organism
UniProt
Commentary
Textmining
Mycobacterium tuberculosis
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-dodecenoyl-acyl carrier protein + NADH
-
687289
Mycobacterium tuberculosis
dodecanoyl-acyl carrier protein + NAD+
-
-
-
?
2-trans,4-trans-decadienoyl-CoA + NADPH + H+
-
687289
Mycobacterium tuberculosis
(4E)-dec-4-enoyl-CoA + NADP+
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
enoyl reductase
-
Mycobacterium tuberculosis
InhA
-
Mycobacterium tuberculosis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.11
-
(4E)-dec-4-enoyl-CoA
mutant enzyme F149A, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)
Mycobacterium tuberculosis
3.17
-
(4E)-dec-4-enoyl-CoA
wild type enzyme, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)
Mycobacterium tuberculosis
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3)pLysS cells
Mycobacterium tuberculosis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Mycobacterium tuberculosis
Engineering (protein specific)
Protein Variants
Commentary
Organism
F149A
the mutant is not able to optimally position the cofactor for hydride transfer, which correlates with the 30fold decrease in kcat
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00433
-
2-dodecenoyl-CoA
mutant enzyme F149A, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)
Mycobacterium tuberculosis
0.0054
-
2-dodecenoyl-CoA
wild type enzyme, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)
Mycobacterium tuberculosis
0.0152
-
NADH
mutant enzyme F149A, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)
Mycobacterium tuberculosis
0.0352
-
NADH
wild type enzyme, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-dodecenoyl-acyl carrier protein + NADH
-
687289
Mycobacterium tuberculosis
dodecanoyl-acyl carrier protein + NAD+
-
-
-
?
2-trans,4-trans-decadienoyl-CoA + NADPH + H+
-
687289
Mycobacterium tuberculosis
(4E)-dec-4-enoyl-CoA + NADP+
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.11
-
(4E)-dec-4-enoyl-CoA
mutant enzyme F149A, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)
Mycobacterium tuberculosis
3.17
-
(4E)-dec-4-enoyl-CoA
wild type enzyme, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)
Mycobacterium tuberculosis
Other publictions for EC 1.3.1.38
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740376
Kim
A novel cytosolic isoform of m ...
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1
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3
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1
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726112
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Characterization of the early ...
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2012
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1
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1
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Gossypium hirsutum
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12
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2
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2
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706445
Gurvitz
A C. elegans model for mitocho ...
Caenorhabditis elegans
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23
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6
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4
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2
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2
2
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-
687289
Bell
Evidence from Raman spectrosco ...
Mycobacterium tuberculosis
J. Am. Chem. Soc.
129
6425-6431
2007
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1
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1
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4
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1
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2
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2
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4
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2
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2
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673644
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Peroxisomal trans-2-enoyl-CoA ...
Homo sapiens
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1
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2
1
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1
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3
1
1
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657270
Poletto
Selection of an Escherichia co ...
Mycobacterium tuberculosis
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34
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3
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6
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1
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656193
Moon
Identification of two mammalia ...
Homo sapiens, Mus musculus
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2
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2
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26
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2
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2
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2
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2
2
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26
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2
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390760
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Molecular cloning and expressi ...
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3
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1
4
1
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2
1
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7
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2
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4
2
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3
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1
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3
3
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1
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4
1
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1
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2
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4
2
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3
1
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390761
Reynolds
-
Comparison of two unusual enoy ...
Streptomyces collinus
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56
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1
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390759
Laguna
Action of Ebselen on rat hepat ...
Rattus norvegicus
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269
272-283
1989
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1
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1
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390758
Nagi
Dual action of 2-decynoyl coen ...
Rattus norvegicus
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267
1-12
1988
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1
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Nagi
Site of inhibition of rat live ...
Rattus norvegicus
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13598-13605
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1
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Prasad
Solubilization and purificatio ...
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237
535-544
1985
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390748
Nishimaki
Studies on the metabolism of u ...
Escherichia coli
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95
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1984
1
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14
6
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2
2
-
8
-
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1
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1
1
8
1
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1
6
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1
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1
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14
6
6
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2
2
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1
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1
1
8
1
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390754
Nagi
Biochemical properties of shor ...
Rattus norvegicus
Arch. Biochem. Biophys.
226
50-64
1983
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390755
Prasad
Kinetic evidence for two separ ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
113
659-665
1983
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1
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1
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1
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390749
Mizugaki
Studies on the metabolism of u ...
Escherichia coli
Chem. Pharm. Bull.
30
2503-2511
1982
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-
-
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5
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2
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1
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1
1
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1
2
5
-
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3
-
1
1
1
1
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1
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5
-
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2
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1
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1
2
5
-
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3
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1
1
1
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390750
Mizugaki
Studies on the metabolism of u ...
Escherichia coli
J. Biochem.
92
1649-1654
1982
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4
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1
1
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1
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1
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1
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1
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1
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1
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390756
Cinti
Evidence for a second microsom ...
Rattus norvegicus
J. Biol. Chem.
257
14333-14340
1982
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1
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1
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4
-
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2
2
-
4
-
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390752
Bernert
The isolation of acyl-CoA deri ...
Rattus norvegicus
Biochim. Biophys. Acta
573
436-442
1979
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390751
Bernert
An analysis of partial reactio ...
Rattus norvegicus
J. Biol. Chem.
252
6736-6744
1977
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