Evidence from Raman spectroscopy that InhA, the mycobacterial enoyl reductase, modulates the conformation of the NADH cofactor to promote catalysis

Bell, A.F.; Stratton, C.F.; Zhang, X.; Novichenok, P.; Jaye, A.A.; Nair, P.A.; Parikh, S.; Rawat, R.; Tonge, P.J.; J. Am. Chem. Soc. 129, 6425-6431 (2007)

Data extracted from this reference:

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.00433

-

2-dodecenoyl-CoA

mutant enzyme F149A, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)

Mycobacterium tuberculosis

0.0054

-

2-dodecenoyl-CoA

wild type enzyme, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)

Mycobacterium tuberculosis

0.0152

-

NADH

mutant enzyme F149A, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)

Mycobacterium tuberculosis

0.0352

-

NADH

wild type enzyme, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)

Mycobacterium tuberculosis

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

2-dodecenoyl-acyl carrier protein + NADH

-

687289

Mycobacterium tuberculosis

dodecanoyl-acyl carrier protein + NAD+

-

-

-

?

2-trans,4-trans-decadienoyl-CoA + NADPH + H+

-

687289

Mycobacterium tuberculosis

(4E)-dec-4-enoyl-CoA + NADP+

-

-

-

?

Turnover Number Minimum [1/s]

Turnover Number Maximum [1/s]

Substrate

Commentary

Organism

Structure

0.11

-

(4E)-dec-4-enoyl-CoA

mutant enzyme F149A, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)

Mycobacterium tuberculosis

3.17

-

(4E)-dec-4-enoyl-CoA

wild type enzyme, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)

Mycobacterium tuberculosis

Cofactor

Commentary

Organism

Structure

NADH

-

Mycobacterium tuberculosis

Cofactor

Commentary

Organism

Structure

NADH

-

Mycobacterium tuberculosis

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.00433

-

2-dodecenoyl-CoA

mutant enzyme F149A, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)

Mycobacterium tuberculosis

0.0054

-

2-dodecenoyl-CoA

wild type enzyme, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)

Mycobacterium tuberculosis

0.0152

-

NADH

mutant enzyme F149A, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)

Mycobacterium tuberculosis

0.0352

-

NADH

wild type enzyme, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)

Mycobacterium tuberculosis

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

2-dodecenoyl-acyl carrier protein + NADH

-

687289

Mycobacterium tuberculosis

dodecanoyl-acyl carrier protein + NAD+

-

-

-

?

2-trans,4-trans-decadienoyl-CoA + NADPH + H+

-

687289

Mycobacterium tuberculosis

(4E)-dec-4-enoyl-CoA + NADP+

-

-

-

?

Turnover Number Minimum [1/s]

Turnover Number Maximum [1/s]

Substrate

Commentary

Organism

Structure

0.11

-

(4E)-dec-4-enoyl-CoA

mutant enzyme F149A, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)

Mycobacterium tuberculosis

3.17

-

(4E)-dec-4-enoyl-CoA

wild type enzyme, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8)

Mycobacterium tuberculosis

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

740376

Kim

A novel cytosolic isoform of m ...

Rattus norvegicus

Endocrinol. Metab. (Seoul)

29

185-194

2014

-

-

1

-

-

-

-

-

3

-

-

1

-

2

-

-

-

-

-

-

-

-

2

-

2

-

-

-

-

-

-

-

1

-

-

-

-

-

1

1

-

-

-

-

-

-

-

3

-

-

1

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

740725

Wakashima

Dual functions of the trans-2- ...

Homo sapiens

J. Biol. Chem.

289

24736-24748

2014

-

-

-

-

-

-

-

-

1

-

-

-

-

5

-

-

-

-

-

1

-

-

1

-

1

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

740717

Abe

Mutation for nonsyndromic ment ...

Homo sapiens

J. Biol. Chem.

288

36741-36749

2013

-

-

1

-

1

-

-

-

-

-

-

1

-

6

-

-

-

-

-

2

-

-

1

-

2

-

-

-

-

-

-

-

1

-

-

-

-

-

1

1

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

2

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

726112

Fu

Characterization of the early ...

Phalaenopsis amabilis

Physiol. Plant.

145

406-425

2012

-

-

1

-

-

-

-

-

-

-

-

-

-

7

-

-

-

-

-

1

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

1

2

2

1

-

-

699263

Song

Characterization of two cotton ...

Gossypium hirsutum

J. Exp. Bot.

60

1839-1848

2009

-

-

-

-

12

-

-

-

-

-

-

-

-

6

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

2

-

12

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

2

-

-

706445

Gurvitz

A C. elegans model for mitocho ...

Caenorhabditis elegans

PLoS ONE

4

e7791

2009

-

-

2

-

2

-

-

-

4

-

-

-

-

23

-

-

-

-

-

-

4

-

2

-

6

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

2

-

-

-

-

-

4

-

-

-

-

-

-

-

-

-

4

-

2

-

-

-

-

-

-

-

-

-

-

2

2

-

-

-

687289

Bell

Evidence from Raman spectrosco ...

Mycobacterium tuberculosis

J. Am. Chem. Soc.

129

6425-6431

2007

-

-

1

-

1

-

-

4

-

-

-

-

-

1

-

-

-

-

-

-

-

-

2

-

2

-

-

-

2

-

-

-

1

-

-

-

-

-

1

1

-

1

-

-

-

-

4

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

673644

Gloerich

Peroxisomal trans-2-enoyl-CoA ...

Homo sapiens

FEBS Lett.

580

2092-2096

2006

-

-

-

-

-

1

-

2

1

-

1

-

-

5

-

-

-

-

-

-

-

-

3

1

1

-

-

-

-

1

-

-

1

-

-

-

-

-

-

1

-

-

1

-

-

-

2

1

-

1

-

-

-

-

-

-

-

-

-

3

1

-

-

-

-

1

-

-

-

-

-

-

-

-

-

657270

Poletto

Selection of an Escherichia co ...

Mycobacterium tuberculosis

Protein Expr. Purif.

34

118-125

2004

-

-

-

-

3

-

-

-

-

-

-

-

-

6

-

-

-

-

-

-

-

-

1

-

2

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

3

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

656193

Moon

Identification of two mammalia ...

Homo sapiens, Mus musculus

J. Biol. Chem.

278

7335-7343

2003

-

-

2

-

-

-

-

-

2

-

-

-

-

2

-

-

-

-

-

26

-

-

2

-

2

-

-

-

-

-

-

-

2

-

-

-

-

-

2

2

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

26

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390760

Das

Molecular cloning and expressi ...

Mus musculus, Homo sapiens, Cavia porcellus

J. Biol. Chem.

275

24333-24340

2000

-

-

3

-

-

-

1

4

1

-

2

1

-

7

-

-

2

-

-

4

2

-

4

3

-

1

-

-

-

-

-

-

3

-

-

-

-

-

3

3

-

-

-

-

1

-

4

1

-

2

1

-

-

-

2

-

4

2

-

4

3

1

-

-

-

-

-

-

-

-

-

-

-

-

-

390761

Reynolds

-

Comparison of two unusual enoy ...

Streptomyces collinus

J. Nat. Prod.

56

175-185

1993

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

1

-

-

-

-

1

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390759

Laguna

Action of Ebselen on rat hepat ...

Rattus norvegicus

Arch. Biochem. Biophys.

269

272-283

1989

-

-

-

-

-

-

-

-

1

-

-

-

-

1

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390758

Nagi

Dual action of 2-decynoyl coen ...

Rattus norvegicus

Arch. Biochem. Biophys.

267

1-12

1988

-

-

-

-

-

-

-

-

1

-

-

-

-

1

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390757

Nagi

Site of inhibition of rat live ...

Rattus norvegicus

J. Biol. Chem.

261

13598-13605

1986

-

-

-

-

-

-

-

-

1

-

-

-

-

2

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390753

Prasad

Solubilization and purificatio ...

Rattus norvegicus

Arch. Biochem. Biophys.

237

535-544

1985

-

-

-

-

-

-

-

-

1

-

-

-

-

3

-

-

-

-

-

2

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

2

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390748

Nishimaki

Studies on the metabolism of u ...

Escherichia coli

J. Biochem.

95

1315-1321

1984

1

-

-

-

-

-

14

6

-

-

2

2

-

8

-

-

1

-

-

-

1

1

8

1

-

-

-

-

-

-

-

-

1

6

-

-

1

-

-

1

-

-

-

-

14

6

6

-

-

2

2

-

-

-

1

-

-

1

1

8

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390754

Nagi

Biochemical properties of shor ...

Rattus norvegicus

Arch. Biochem. Biophys.

226

50-64

1983

-

-

-

-

-

-

-

-

1

-

-

-

-

1

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390755

Prasad

Kinetic evidence for two separ ...

Rattus norvegicus

Biochem. Biophys. Res. Commun.

113

659-665

1983

-

-

-

-

-

-

-

-

1

-

-

-

-

1

-

-

-

-

-

1

2

-

3

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

2

-

3

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

390749

Mizugaki

Studies on the metabolism of u ...

Escherichia coli

Chem. Pharm. Bull.

30

2503-2511

1982

-

-

-

-

-

-

5

-

-

-

-

2

-

1

-

-

1

1

-

-

1

2

5

-

-

-

-

3

-

1

1

1

1

-

-

-

-

-

-

1

-

-

-

-

5

-

-

-

-

-

2

-

-

-

1

-

-

1

2

5

-

-

-

3

-

1

1

1

-

-

-

-

-

-

-

390750

Mizugaki

Studies on the metabolism of u ...

Escherichia coli

J. Biochem.

92

1649-1654

1982

-

-

-

-

-

-

-

-

-

-

-

-

-

4

-

-

1

1

-

-

-

-

1

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

390756

Cinti

Evidence for a second microsom ...

Rattus norvegicus

J. Biol. Chem.

257

14333-14340

1982

-

-

-

-

-

-

-

-

1

-

-

1

-

4

-

-

-

-

-

2

2

-

4

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

2

2

-

4

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390752

Bernert

The isolation of acyl-CoA deri ...

Rattus norvegicus

Biochim. Biophys. Acta

573

436-442

1979

-

-

-

-

-

-

-

-

1

-

-

-

-

1

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

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390751

Bernert

An analysis of partial reactio ...

Rattus norvegicus

J. Biol. Chem.

252

6736-6744

1977

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