Cloned (Comment) | Organism |
---|---|
functional overexpression of truncated His-tagged enzyme in Escherichia coli strain BL21(DE3) | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0062 | - |
2,4-trans-decadienoyl-CoA | recombinant His-tagged truncated enzyme, pH 6.0, 22°C | Homo sapiens | |
0.0265 | - |
2,4-trans-hexadienoyl-CoA | recombinant His-tagged truncated enzyme, pH 6.0, 22°C | Homo sapiens | |
0.0605 | - |
NADPH | recombinant His-tagged truncated enzyme, pH 6.0, 22°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, recombinant truncated His-tagged enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | key enzyme in the beta-oxidation of unsaturated fatty acids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged truncated enzyme from Escherichia coli strain BL21(DE3) by one-step nickel affinity chromatography, to over 95% purity | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.21 | - |
purified recombinant truncated His-tagged enzyme, substrate 2,4-trans-hexadienoyl-CoA | Homo sapiens |
Storage Stability | Organism |
---|---|
-80°C, purified recombinant truncated enzyme, completely stable for at least 6 months | Homo sapiens |
22°C, room temperature, purified recombinant truncated enzyme, 70% remaining activity after 1 week | Homo sapiens |
4°C, purified recombinant truncated enzyme, completely stable for 1 week | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-trans-decadienoyl-CoA + NADPH | - |
Homo sapiens | ? + NADP+ | - |
? | |
2,4-trans-hexadienoyl-CoA + NADPH | - |
Homo sapiens | trans-3-hexenoyl-CoA + NADP+ | - |
? | |
additional information | key enzyme in the beta-oxidation of unsaturated fatty acids | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, recombinant truncated His-tagged enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
2,4-dienoyl-CoA reductase | - |
Homo sapiens |
DECR | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
purified recombinant truncated enzyme is highly stable at room temperature | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |