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Literature summary for 1.3.1.34 extracted from

  • Chu, X.; Yu, W.; Chen, G.; Li, D.
    Expression, purification, and characterization of His-tagged human mitochondrial 2,4-dienoyl-CoA reductase (2003), Protein Expr. Purif., 31, 292-297.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
functional overexpression of truncated His-tagged enzyme in Escherichia coli strain BL21(DE3) Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0062
-
2,4-trans-decadienoyl-CoA recombinant His-tagged truncated enzyme, pH 6.0, 22°C Homo sapiens
0.0265
-
2,4-trans-hexadienoyl-CoA recombinant His-tagged truncated enzyme, pH 6.0, 22°C Homo sapiens
0.0605
-
NADPH recombinant His-tagged truncated enzyme, pH 6.0, 22°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Homo sapiens 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
x * 34000, recombinant truncated His-tagged enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens key enzyme in the beta-oxidation of unsaturated fatty acids ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged truncated enzyme from Escherichia coli strain BL21(DE3) by one-step nickel affinity chromatography, to over 95% purity Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.21
-
purified recombinant truncated His-tagged enzyme, substrate 2,4-trans-hexadienoyl-CoA Homo sapiens

Storage Stability

Storage Stability Organism
-80°C, purified recombinant truncated enzyme, completely stable for at least 6 months Homo sapiens
22°C, room temperature, purified recombinant truncated enzyme, 70% remaining activity after 1 week Homo sapiens
4°C, purified recombinant truncated enzyme, completely stable for 1 week Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-trans-decadienoyl-CoA + NADPH
-
Homo sapiens ? + NADP+
-
?
2,4-trans-hexadienoyl-CoA + NADPH
-
Homo sapiens trans-3-hexenoyl-CoA + NADP+
-
?
additional information key enzyme in the beta-oxidation of unsaturated fatty acids Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
? x * 34000, recombinant truncated His-tagged enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
2,4-dienoyl-CoA reductase
-
Homo sapiens
DECR
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
purified recombinant truncated enzyme is highly stable at room temperature Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Homo sapiens