Protein Variants | Comment | Organism |
---|---|---|
F233L | mutant enzyme shows around 40% of wild-type activity | Thermosynechococcus vestitus |
F233Y | mutant enzyme shows around 40% of wild-type activity | Thermosynechococcus vestitus |
F237Y | as in the wild-type enzyme the visible spectrum of protochlorophyllide bound to the mutant enzyme is red-shifted by about 12 nm compared to free protochlorophyllide | Thermosynechococcus vestitus |
F240Y | distinct from the wild-type enzyme the visible spectrum of protochlorophyllide bound to the mutant enzyme is not red-shifted as compared to free protochlorophyllide | Thermosynechococcus vestitus |
F244Y | distinct from the wild-type enzyme the visible spectrum of protochlorophyllide bound to the mutant enzyme is not red-shifted as compared to free protochlorophyllide | Thermosynechococcus vestitus |
F247Y | distinct from the wild-type enzyme the visible spectrum of protochlorophyllide bound to the mutant enzyme is not red-shifted as compared to free protochlorophyllide | Thermosynechococcus vestitus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
NADPH | pH 7.5, 40°C, mutant enzyme P158F | Thermosynechococcus vestitus | |
0.0009 | - |
NADPH | pH 7.5, 40°C, wild-type enzyme | Thermosynechococcus vestitus | |
0.001 | - |
NADPH | pH 7.5, 40°C, mutant enzyme F233Y | Thermosynechococcus vestitus | |
0.0022 | - |
NADPH | pH 7.5, 40°C, mutant enzyme K156N/I157V | Thermosynechococcus vestitus | |
0.0025 | - |
NADPH | pH 7.5, 40°C, mutant enzyme F237Y | Thermosynechococcus vestitus | |
0.0029 | - |
NADPH | pH 7.5, 40°C, mutant enzyme F233L | Thermosynechococcus vestitus | |
0.0029 | - |
NADPH | pH 7.5, 40°C, mutant enzyme I157V | Thermosynechococcus vestitus | |
0.0029 | - |
NADPH | pH 7.5, 40°C, mutant enzyme K156N | Thermosynechococcus vestitus | |
0.0033 | - |
NADPH | pH 7.5, 40°C, mutant enzyme P158A | Thermosynechococcus vestitus | |
0.0069 | - |
protochlorophyllide | pH 7.5, 40°C, mutant enzyme F237Y | Thermosynechococcus vestitus | |
0.0075 | - |
protochlorophyllide | pH 7.5, 40°C, mutant enzyme F233L | Thermosynechococcus vestitus | |
0.0078 | - |
protochlorophyllide | pH 7.5, 40°C, wild-type enzyme | Thermosynechococcus vestitus | |
0.0081 | - |
protochlorophyllide | pH 7.5, 40°C, mutant enzyme F233Y | Thermosynechococcus vestitus | |
0.0107 | - |
protochlorophyllide | pH 7.5, 40°C, mutant enzyme I157V | Thermosynechococcus vestitus | |
0.0135 | - |
protochlorophyllide | pH 7.5, 40°C, mutant enzyme P158A | Thermosynechococcus vestitus | |
0.0151 | - |
protochlorophyllide | pH 7.5, 40°C, mutant enzyme K156N | Thermosynechococcus vestitus | |
0.0166 | - |
protochlorophyllide | pH 7.5, 40°C, mutant enzyme K156N/I157V | Thermosynechococcus vestitus | |
0.0181 | - |
protochlorophyllide | pH 7.5, 40°C, mutant enzyme P158F | Thermosynechococcus vestitus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protochlorophyllide + NADPH + H+ | Thermosynechococcus vestitus | light-dependent reaction of chlorophyll biosynthesis | chlorophyllide a + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermosynechococcus vestitus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protochlorophyllide + NADPH + H+ | light-dependent reaction of chlorophyll biosynthesis | Thermosynechococcus vestitus | chlorophyllide a + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | protochlorophyllide binding triggers formation of large oligomers of the enzyme (POR). Oligomer formation is most likely driven by the interaction of amino acid residues in the highly conserved lid regions. The lid residues position protochlorophyllide optimally to enable photocatalysis. Following protochlorophyllide binding, they also enable POR oligomerisation - a process that is reversed through subsequent photocatalysis in the early stages of chloroplast development | Thermosynechococcus vestitus |
Synonyms | Comment | Organism |
---|---|---|
POR | - |
Thermosynechococcus vestitus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
protochlorophyllide | pH 7.5, 40°C, mutant enzyme F233L | Thermosynechococcus vestitus | |
0.013 | - |
protochlorophyllide | pH 7.5, 40°C, mutant enzyme F233Y | Thermosynechococcus vestitus | |
0.013 | - |
protochlorophyllide | pH 7.5, 40°C, mutant enzyme P158F | Thermosynechococcus vestitus | |
0.022 | - |
protochlorophyllide | pH 7.5, 40°C, mutant enzyme K156N/I157V | Thermosynechococcus vestitus | |
0.027 | - |
protochlorophyllide | pH 7.5, 40°C, wild-type enzyme | Thermosynechococcus vestitus | |
0.03 | - |
protochlorophyllide | pH 7.5, 40°C, mutant enzyme F237Y | Thermosynechococcus vestitus | |
0.035 | - |
protochlorophyllide | pH 7.5, 40°C, mutant enzyme K156N | Thermosynechococcus vestitus | |
0.037 | - |
protochlorophyllide | pH 7.5, 40°C, mutant enzyme I157V | Thermosynechococcus vestitus | |
0.037 | - |
protochlorophyllide | pH 7.5, 40°C, mutant enzyme P158A | Thermosynechococcus vestitus |
General Information | Comment | Organism |
---|---|---|
physiological function | light-dependent reaction of chlorophyll biosynthesis. The enzyme (POR) is also important in plant development as it is the main constituent of prolamellar bodies in etioplast membranes | Thermosynechococcus vestitus |