Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermosynechococcus vestitus |
Protein Variants | Comment | Organism |
---|---|---|
G19A | the mutant shows wild type value for turnover number | Thermosynechococcus vestitus |
H236A | the mutant shows reduced value for turnover number compared to the wild type enzyme | Thermosynechococcus vestitus |
K42A | the mutant shows wild type value for turnover number | Thermosynechococcus vestitus |
N149V | the mutant shows reduced value for turnover number compared to the wild type enzyme | Thermosynechococcus vestitus |
N39V | the mutant shows reduced value for turnover number compared to the wild type enzyme | Thermosynechococcus vestitus |
N90A | the mutant shows reduced value for turnover number compared to the wild type enzyme | Thermosynechococcus vestitus |
R38V | the mutant shows wild type value for turnover number | Thermosynechococcus vestitus |
S16C | the mutant shows wild type value for turnover number | Thermosynechococcus vestitus |
S189A | the mutant shows wild type value for turnover number | Thermosynechococcus vestitus |
T145A | the mutant shows reduced value for turnover number compared to the wild type enzyme | Thermosynechococcus vestitus |
T147F | the mutant shows reduced value for turnover number compared to the wild type enzyme | Thermosynechococcus vestitus |
T147S | the mutant shows reduced value for turnover number compared to the wild type enzyme | Thermosynechococcus vestitus |
T230A | the mutant shows wild type value for turnover number | Thermosynechococcus vestitus |
T230F | the mutant shows reduced value for turnover number compared to the wild type enzyme | Thermosynechococcus vestitus |
T230S | the mutant shows wild type value for turnover number | Thermosynechococcus vestitus |
Y94F | the mutant shows wild type value for turnover number | Thermosynechococcus vestitus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protochlorophyllide + NADPH + H+ | Thermosynechococcus vestitus | - |
chlorophyllide a + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermosynechococcus vestitus | Q8DLC1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protochlorophyllide + NADPH + H+ | - |
Thermosynechococcus vestitus | chlorophyllide a + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
light-activated enzyme protochlorophyllide oxidoreductase | - |
Thermosynechococcus vestitus |
POR | - |
Thermosynechococcus vestitus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
protochlorophyllide | mutant enzyme N149V, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
0.01 | - |
protochlorophyllide | mutant enzyme T145A, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
0.01 | - |
protochlorophyllide | mutant enzyme T147F, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
0.01 | - |
protochlorophyllide | mutant enzyme T230F, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
0.02 | - |
protochlorophyllide | mutant enzyme N39V, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
0.02 | - |
protochlorophyllide | mutant enzyme T147S, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
0.07 | - |
protochlorophyllide | mutant enzyme N90A, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
0.1 | - |
protochlorophyllide | mutant enzyme H236A, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
0.16 | - |
protochlorophyllide | mutant enzyme G19A, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
0.16 | - |
protochlorophyllide | mutant enzyme K42A, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
0.16 | - |
protochlorophyllide | mutant enzyme R38V, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
0.16 | - |
protochlorophyllide | mutant enzyme S16C, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
0.16 | - |
protochlorophyllide | mutant enzyme S189A, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
0.16 | - |
protochlorophyllide | mutant enzyme T230A, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
0.16 | - |
protochlorophyllide | mutant enzyme T230S, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
0.17 | - |
protochlorophyllide | wild type enzyme, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
0.17 | - |
protochlorophyllide | mutant enzyme Y94F, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Thermosynechococcus vestitus |