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Literature summary for 1.3.1.33 extracted from

  • Menon, B.R.; Davison, P.A.; Hunter, C.N.; Scrutton, N.S.; Heyes, D.J.
    Mutagenesis alters the catalytic mechanism of the light-driven enzyme protochlorophyllide oxidoreductase (2009), J. Biol. Chem., 285, 2113-2119.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Thermosynechococcus vestitus
expression in Escherichia coli cells Thermosynechococcus vestitus

Protein Variants

Protein Variants Comment Organism
C199/C226S mutant, mutation of the absolutely conserved cysteine residues Thermosynechococcus vestitus
C199S mutant, Cys199 has a relatively minor role in catalysis Thermosynechococcus vestitus
C199S kcat comparable to wild-type, relative activity comparable to wild-type, Kd (NADPH) comparable to wild-type, Kd (protochlorophyllide) comparable to wild-type Thermosynechococcus vestitus
C199S/C226S kcat more that 10fold decreased to wild-type, relative activity highly decreased compared to wild-type, Kd (NADPH) 2fold increased compared to wild-type, Kd (protochlorophyllide) 6fold increased compared to wild-type Thermosynechococcus vestitus
C226S mutant, mutation causes a remarkable change in the mechansim of the hydrogen transfer reactions Thermosynechococcus vestitus
C226S kcat more that 10fold decreased to wild-type, relative activity highly decreased compared to wild-type, Kd (NADPH) comparable to wild-type, Kd (protochlorophyllide) 4fold increased compared to wild-type Thermosynechococcus vestitus
C37S mutant, Cys37 has a relatively minor role in catalysis Thermosynechococcus vestitus
C37S kcat comparable to wild-type, relative activity comparable to wild-type, Kd (NADPH) increased compared to wild-type, Kd (protochlorophyllide) equal to wild-type Thermosynechococcus vestitus
C37S/C199S mutant, mutation of the absolutely conserved cysteine residues Thermosynechococcus vestitus
C37S/C199S kcat comparable to wild-type, relative activity comparable to wild-type, Kd (NADPH) 10fold increased compared to wild-type, Kd (protochlorophyllide) comparable to wild-type Thermosynechococcus vestitus
C37S/C199S/C226S mutant, mutation of the absolutely conserved cysteine residues Thermosynechococcus vestitus
C37S/C199S/C226S kcat more that 10fold decreased to wild-type, relative activity highly decreased compared to wild-type, Kd (NADPH) 10fold increased compared to wild-type, Kd (protochlorophyllide) 6fold increased compared to wild-type Thermosynechococcus vestitus
C37S/C226S mutant, mutation of the absolutely conserved cysteine residues Thermosynechococcus vestitus
C37S/C226S kcat more that 10fold decreased to wild-type, relative activity highly decreased compared to wild-type, Kd (NADPH) 10fold increased compared to wild-type, Kd (protochlorophyllide) 6fold increased compared to wild-type Thermosynechococcus vestitus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
protochlorophyllide mutant C199/C226S, relative activity 6.6% Thermosynechococcus vestitus
additional information
-
protochlorophyllide mutant C199S, relative activity 98.2% Thermosynechococcus vestitus
additional information
-
protochlorophyllide mutant C226S, relative activity 7.2% Thermosynechococcus vestitus
additional information
-
protochlorophyllide mutant C37S, relative activity 99.4% Thermosynechococcus vestitus
additional information
-
protochlorophyllide mutant C37S/C199S, relative activity 98.8% Thermosynechococcus vestitus
additional information
-
protochlorophyllide mutant C37S/C199S/C226S, relative activity 7.1% Thermosynechococcus vestitus
additional information
-
protochlorophyllide mutant C37S/C226S, relative activity 6.8% Thermosynechococcus vestitus
additional information
-
protochlorophyllide wild type enzyme, relative activity 100% Thermosynechococcus vestitus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protochlorophyllide + NADPH Thermosynechococcus vestitus
-
chlorophyllide + NADP+
-
?
protochlorophyllide + NADPH + H+ Thermosynechococcus vestitus activation by light chlorophyllide + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Thermosynechococcus vestitus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protochlorophyllide + NADPH
-
Thermosynechococcus vestitus chlorophyllide + NADP+
-
?
protochlorophyllide + NADPH + H+ activation by light Thermosynechococcus vestitus chlorophyllide + NADP+
-
?

Synonyms

Synonyms Comment Organism
POR
-
Thermosynechococcus vestitus
protochlorophyllide oxidoreductase
-
Thermosynechococcus vestitus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Thermosynechococcus vestitus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.011
-
protochlorophyllide mutant C199/C226S Thermosynechococcus vestitus
0.011
-
protochlorophyllide pH 7.5, 25°C, mutant C199S/C226S, co-substrate: NADPH Thermosynechococcus vestitus
0.011
-
NADPH pH 7.5, 25°C, mutant C199S/C226S, co-substrate: protochlorophyllide Thermosynechococcus vestitus
0.0113
-
protochlorophyllide mutant C37S/C226S Thermosynechococcus vestitus
0.0113
-
protochlorophyllide pH 7.5, 25°C, mutant C37S/C226S, co-substrate: NADPH Thermosynechococcus vestitus
0.0113
-
NADPH pH 7.5, 25°C, mutant C37S/C226S, co-substrate: protochlorophyllide Thermosynechococcus vestitus
0.0117
-
protochlorophyllide mutant C37S/C199S/C226S Thermosynechococcus vestitus
0.0117
-
protochlorophyllide pH 7.5, 25°C, mutant C37S/C199S/C226S, co-substrate: NADPH Thermosynechococcus vestitus
0.0117
-
NADPH pH 7.5, 25°C, mutant C37S/C199S/C226S, co-substrate: protochlorophyllide Thermosynechococcus vestitus
0.0118
-
protochlorophyllide mutant C226S Thermosynechococcus vestitus
0.0118
-
protochlorophyllide pH 7.5, 25°C, mutant C226S, co-substrate: NADPH Thermosynechococcus vestitus
0.0118
-
NADPH pH 7.5, 25°C, mutant C226S, co-substrate: protochlorophyllide Thermosynechococcus vestitus
0.162
-
protochlorophyllide mutant C199S Thermosynechococcus vestitus
0.162
-
protochlorophyllide pH 7.5, 25°C, mutant C199S, co-substrate: NADPH Thermosynechococcus vestitus
0.162
-
NADPH pH 7.5, 25°C, mutant C199S, co-substrate: protochlorophyllide Thermosynechococcus vestitus
0.163
-
protochlorophyllide mutant C37S/C199S Thermosynechococcus vestitus
0.164
-
protochlorophyllide mutant C37S Thermosynechococcus vestitus
0.164
-
protochlorophyllide pH 7.5, 25°C, mutant C37S, co-substrate: NADPH Thermosynechococcus vestitus
0.164
-
NADPH pH 7.5, 25°C, mutant C37S, co-substrate: protochlorophyllide Thermosynechococcus vestitus
0.165
-
protochlorophyllide wild type enzyme Thermosynechococcus vestitus
0.165
-
protochlorophyllide pH 7.5, 25°C, wild-type, co-substrate: NADPH Thermosynechococcus vestitus
0.165
-
NADPH pH 7.5, 25°C, wild-type, co-substrate: protochlorophyllide Thermosynechococcus vestitus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Thermosynechococcus vestitus
7.5
-
activity assay Thermosynechococcus vestitus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Thermosynechococcus vestitus