BRENDA - Enzyme Database show
show all sequences of 1.3.1.32

Purification and characterization of maleylacetate reductase from Alcaligenes eutrophus JMP134(pJP4)

Seibert, V.; Stadler-Fritzsche, K.; Schloemann, M.; J. Bacteriol. 175, 6745-6754 (1993)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
histidine
histidine-HCl buffer results in considerably higher activity than corresponding bisTris-HCl or Tris-HCl buffers
Cupriavidus necator
Cloned(Commentary)
Commentary
Organism
-
Pseudomonas sp.
General Stability
General Stability
Organism
addition of DTT significantly stabilized enzyme
Cupriavidus necator
Inhibitors
Inhibitors
Commentary
Organism
Structure
3-oxoadipate
competitive inhibition
Cupriavidus necator
Mn2+
-
Cupriavidus necator
MnSO4
-
Cupriavidus necator
p-chloromercuribenzoate
-
Cupriavidus necator
p-chloromercuribenzoate
-
Cutaneotrichosporon cutaneum
p-chloromercuribenzoate
-
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.024
-
NADH
2-chloromaleylacetate as substrate
Cupriavidus necator
0.031
-
2-chloromaleylacetate
-
Cupriavidus necator
0.031
-
maleylacetate
-
Cupriavidus necator
0.041
-
NADH
maleylacetate as substrate
Cupriavidus necator
0.082
-
NADPH
2-chloromaleylacetate as substrate
Cupriavidus necator
0.089
-
NADPH
maleylacetate as substrate
Cupriavidus necator
0.09
-
maleylacetate
NADPH as cosubstrate
Cupriavidus necator
0.132
-
2-chloromaleylacetate
NADPH as cosubstrate
Cupriavidus necator
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
18000
-
strain PKO1
Pseudomonas sp.
35000
-
2 * 35000, SDS-PAGE
Cupriavidus necator
40000
-
2 * 40000, SDS-PAGE
Cutaneotrichosporon cutaneum
56000
-
gel filtration, Superdex 200
Cupriavidus necator
72000
-
analytical gel filtration
Cupriavidus necator
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-maleylacetate + NADPH
Pseudomonas sp.
-
3-oxoadipate + NADP+
-
-
ir
2-maleylacetate + NADPH
Cupriavidus necator
-
3-oxoadipate + NADP+
-
-
ir
2-maleylacetate + NADPH
Cutaneotrichosporon cutaneum
-
3-oxoadipate + NADP+
-
-
ir
2-maleylacetate + NADPH
Sporotrichum pulverulentum
-
3-oxoadipate + NADP+
-
-
ir
2-maleylacetate + NADPH
Pseudomonas sp. B13
-
3-oxoadipate + NADP+
-
-
ir
2-maleylacetate + NADPH
Cupriavidus necator JMP 134-1
-
3-oxoadipate + NADP+
-
-
ir
2-maleylacetate + NADPH
Pseudomonas sp. PKO1
-
3-oxoadipate + NADP+
-
-
ir
2-maleylacetate + NADPH
Cupriavidus necator JMP 134(pJP4)
-
3-oxoadipate + NADP+
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cupriavidus necator
-
JMP 134; JMP 134(pJP4)
-
Cupriavidus necator JMP 134(pJP4)
-
JMP 134(pJP4)
-
Cupriavidus necator JMP 134-1
-
JMP 134
-
Cutaneotrichosporon cutaneum
-
-
-
Pseudomonas sp.
-
-
-
Pseudomonas sp. B13
-
-
-
Pseudomonas sp. PKO1
-
-
-
Sporotrichum pulverulentum
-
lignin-degrading white-rot fungus
-
Purification (Commentary)
Commentary
Organism
-
Cutaneotrichosporon cutaneum
-
Pseudomonas sp.
-
Cupriavidus necator
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
600
-
-
Cupriavidus necator
Storage Stability
Storage Stability
Organism
-23C, purified enzyme in 50 mM Tris-HCl, pH 7.5, 0.5 mM DTT, 0.1 mM EDTA after freezing, storage overnight and rethawing 35% loss of activity
Cupriavidus necator
4C, purified enzyme can be stored in 50 mM Tris-HCl, pH 7.5, 0.5 mM DTT, 0.1 mM EDTA with slight loss of activity, 36% in 3 weeks
Cupriavidus necator
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-chloromaleylacetate + NADPH
reductive dehalogenation
390700
Cupriavidus necator
2-maleylacetate + Cl- + NADP+
-
-
-
?
2-chloromaleylacetate + NADPH
reductive dehalogenation
390700
Cupriavidus necator JMP 134-1
2-maleylacetate + Cl- + NADP+
-
-
-
?
2-chloromaleylacetate + NADPH
reductive dehalogenation
390700
Cupriavidus necator JMP 134(pJP4)
2-maleylacetate + Cl- + NADP+
-
-
-
?
2-maleylacetate + NAD(P)H
-
390700
Pseudomonas sp.
3-oxoadipate + NAD(P)+
-
-
-
ir
2-maleylacetate + NAD(P)H
-
390700
Cupriavidus necator
3-oxoadipate + NAD(P)+
-
-
-
ir
2-maleylacetate + NAD(P)H
-
390700
Cutaneotrichosporon cutaneum
3-oxoadipate + NAD(P)+
-
-
-
ir
2-maleylacetate + NAD(P)H
-
390700
Sporotrichum pulverulentum
3-oxoadipate + NAD(P)+
-
-
-
ir
2-maleylacetate + NAD(P)H
-
390700
Pseudomonas sp. B13
3-oxoadipate + NAD(P)+
-
-
-
ir
2-maleylacetate + NAD(P)H
-
390700
Cupriavidus necator JMP 134-1
3-oxoadipate + NAD(P)+
-
-
-
ir
2-maleylacetate + NAD(P)H
-
390700
Pseudomonas sp. PKO1
3-oxoadipate + NAD(P)+
-
-
-
ir
2-maleylacetate + NAD(P)H
-
390700
Cupriavidus necator JMP 134(pJP4)
3-oxoadipate + NAD(P)+
-
-
-
ir
2-maleylacetate + NADPH
-
390700
Pseudomonas sp.
3-oxoadipate + NADP+
-
-
-
ir
2-maleylacetate + NADPH
-
390700
Cupriavidus necator
3-oxoadipate + NADP+
-
-
-
ir
2-maleylacetate + NADPH
-
390700
Cutaneotrichosporon cutaneum
3-oxoadipate + NADP+
-
-
-
ir
2-maleylacetate + NADPH
-
390700
Sporotrichum pulverulentum
3-oxoadipate + NADP+
-
-
-
ir
2-maleylacetate + NADPH
-
390700
Pseudomonas sp. B13
3-oxoadipate + NADP+
-
-
-
ir
2-maleylacetate + NADPH
-
390700
Cupriavidus necator JMP 134-1
3-oxoadipate + NADP+
-
-
-
ir
2-maleylacetate + NADPH
-
390700
Pseudomonas sp. PKO1
3-oxoadipate + NADP+
-
-
-
ir
2-maleylacetate + NADPH
-
390700
Cupriavidus necator JMP 134(pJP4)
3-oxoadipate + NADP+
-
-
-
ir
cis,cis-3-hydroxymuconate + NADPH
-
390700
Cupriavidus necator
? + NADP+
-
-
-
?
cis,cis-3-hydroxymuconate + NADPH
-
390700
Cupriavidus necator JMP 134-1
? + NADP+
-
-
-
?
cis,cis-3-hydroxymuconate + NADPH
-
390700
Cupriavidus necator JMP 134(pJP4)
? + NADP+
-
-
-
?
trans,cis-3-hydroxymuconate + NADPH
-
390700
Cupriavidus necator
? + NADP+
-
-
-
?
trans,cis-3-hydroxymuconate + NADPH
-
390700
Cupriavidus necator JMP 134-1
? + NADP+
-
-
-
?
trans,cis-3-hydroxymuconate + NADPH
-
390700
Cupriavidus necator JMP 134(pJP4)
? + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 35000, SDS-PAGE
Cupriavidus necator
dimer
2 * 40000, SDS-PAGE
Cutaneotrichosporon cutaneum
dimer
-
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7.8
-
Cutaneotrichosporon cutaneum
7.5
-
-
Cupriavidus necator
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
8.5
-
Cupriavidus necator
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
8.5
-
Cupriavidus necator
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
NADH is preferred to NADPH
Cupriavidus necator
NADH
-
Cutaneotrichosporon cutaneum
NADH
-
Pseudomonas sp.
NADPH
-
Pseudomonas sp.
NADPH
-
Sporotrichum pulverulentum
NADPH
-
Cutaneotrichosporon cutaneum
NADPH
-
Cupriavidus necator
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.8
-
3-oxoadipate
-
Cupriavidus necator
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
histidine
histidine-HCl buffer results in considerably higher activity than corresponding bisTris-HCl or Tris-HCl buffers
Cupriavidus necator
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
NADH is preferred to NADPH
Cupriavidus necator
NADH
-
Cutaneotrichosporon cutaneum
NADH
-
Pseudomonas sp.
NADPH
-
Pseudomonas sp.
NADPH
-
Sporotrichum pulverulentum
NADPH
-
Cutaneotrichosporon cutaneum
NADPH
-
Cupriavidus necator
General Stability (protein specific)
General Stability
Organism
addition of DTT significantly stabilized enzyme
Cupriavidus necator
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-oxoadipate
competitive inhibition
Cupriavidus necator
Mn2+
-
Cupriavidus necator
MnSO4
-
Cupriavidus necator
p-chloromercuribenzoate
-
Cupriavidus necator
p-chloromercuribenzoate
-
Cutaneotrichosporon cutaneum
p-chloromercuribenzoate
-
Pseudomonas sp.
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.8
-
3-oxoadipate
-
Cupriavidus necator
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.024
-
NADH
2-chloromaleylacetate as substrate
Cupriavidus necator
0.031
-
2-chloromaleylacetate
-
Cupriavidus necator
0.031
-
maleylacetate
-
Cupriavidus necator
0.041
-
NADH
maleylacetate as substrate
Cupriavidus necator
0.082
-
NADPH
2-chloromaleylacetate as substrate
Cupriavidus necator
0.089
-
NADPH
maleylacetate as substrate
Cupriavidus necator
0.09
-
maleylacetate
NADPH as cosubstrate
Cupriavidus necator
0.132
-
2-chloromaleylacetate
NADPH as cosubstrate
Cupriavidus necator
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
18000
-
strain PKO1
Pseudomonas sp.
35000
-
2 * 35000, SDS-PAGE
Cupriavidus necator
40000
-
2 * 40000, SDS-PAGE
Cutaneotrichosporon cutaneum
56000
-
gel filtration, Superdex 200
Cupriavidus necator
72000
-
analytical gel filtration
Cupriavidus necator
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-maleylacetate + NADPH
Pseudomonas sp.
-
3-oxoadipate + NADP+
-
-
ir
2-maleylacetate + NADPH
Cupriavidus necator
-
3-oxoadipate + NADP+
-
-
ir
2-maleylacetate + NADPH
Cutaneotrichosporon cutaneum
-
3-oxoadipate + NADP+
-
-
ir
2-maleylacetate + NADPH
Sporotrichum pulverulentum
-
3-oxoadipate + NADP+
-
-
ir
2-maleylacetate + NADPH
Pseudomonas sp. B13
-
3-oxoadipate + NADP+
-
-
ir
2-maleylacetate + NADPH
Cupriavidus necator JMP 134-1
-
3-oxoadipate + NADP+
-
-
ir
2-maleylacetate + NADPH
Pseudomonas sp. PKO1
-
3-oxoadipate + NADP+
-
-
ir
2-maleylacetate + NADPH
Cupriavidus necator JMP 134(pJP4)
-
3-oxoadipate + NADP+
-
-
ir
Purification (Commentary) (protein specific)
Commentary
Organism
-
Cutaneotrichosporon cutaneum
-
Pseudomonas sp.
-
Cupriavidus necator
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
600
-
-
Cupriavidus necator
Storage Stability (protein specific)
Storage Stability
Organism
-23C, purified enzyme in 50 mM Tris-HCl, pH 7.5, 0.5 mM DTT, 0.1 mM EDTA after freezing, storage overnight and rethawing 35% loss of activity
Cupriavidus necator
4C, purified enzyme can be stored in 50 mM Tris-HCl, pH 7.5, 0.5 mM DTT, 0.1 mM EDTA with slight loss of activity, 36% in 3 weeks
Cupriavidus necator
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-chloromaleylacetate + NADPH
reductive dehalogenation
390700
Cupriavidus necator
2-maleylacetate + Cl- + NADP+
-
-
-
?
2-chloromaleylacetate + NADPH
reductive dehalogenation
390700
Cupriavidus necator JMP 134-1
2-maleylacetate + Cl- + NADP+
-
-
-
?
2-chloromaleylacetate + NADPH
reductive dehalogenation
390700
Cupriavidus necator JMP 134(pJP4)
2-maleylacetate + Cl- + NADP+
-
-
-
?
2-maleylacetate + NAD(P)H
-
390700
Pseudomonas sp.
3-oxoadipate + NAD(P)+
-
-
-
ir
2-maleylacetate + NAD(P)H
-
390700
Cupriavidus necator
3-oxoadipate + NAD(P)+
-
-
-
ir
2-maleylacetate + NAD(P)H
-
390700
Cutaneotrichosporon cutaneum
3-oxoadipate + NAD(P)+
-
-
-
ir
2-maleylacetate + NAD(P)H
-
390700
Sporotrichum pulverulentum
3-oxoadipate + NAD(P)+
-
-
-
ir
2-maleylacetate + NAD(P)H
-
390700
Pseudomonas sp. B13
3-oxoadipate + NAD(P)+
-
-
-
ir
2-maleylacetate + NAD(P)H
-
390700
Cupriavidus necator JMP 134-1
3-oxoadipate + NAD(P)+
-
-
-
ir
2-maleylacetate + NAD(P)H
-
390700
Pseudomonas sp. PKO1
3-oxoadipate + NAD(P)+
-
-
-
ir
2-maleylacetate + NAD(P)H
-
390700
Cupriavidus necator JMP 134(pJP4)
3-oxoadipate + NAD(P)+
-
-
-
ir
2-maleylacetate + NADPH
-
390700
Pseudomonas sp.
3-oxoadipate + NADP+
-
-
-
ir
2-maleylacetate + NADPH
-
390700
Cupriavidus necator
3-oxoadipate + NADP+
-
-
-
ir
2-maleylacetate + NADPH
-
390700
Cutaneotrichosporon cutaneum
3-oxoadipate + NADP+
-
-
-
ir
2-maleylacetate + NADPH
-
390700
Sporotrichum pulverulentum
3-oxoadipate + NADP+
-
-
-
ir
2-maleylacetate + NADPH
-
390700
Pseudomonas sp. B13
3-oxoadipate + NADP+
-
-
-
ir
2-maleylacetate + NADPH
-
390700
Cupriavidus necator JMP 134-1
3-oxoadipate + NADP+
-
-
-
ir
2-maleylacetate + NADPH
-
390700
Pseudomonas sp. PKO1
3-oxoadipate + NADP+
-
-
-
ir
2-maleylacetate + NADPH
-
390700
Cupriavidus necator JMP 134(pJP4)
3-oxoadipate + NADP+
-
-
-
ir
cis,cis-3-hydroxymuconate + NADPH
-
390700
Cupriavidus necator
? + NADP+
-
-
-
?
cis,cis-3-hydroxymuconate + NADPH
-
390700
Cupriavidus necator JMP 134-1
? + NADP+
-
-
-
?
cis,cis-3-hydroxymuconate + NADPH
-
390700
Cupriavidus necator JMP 134(pJP4)
? + NADP+
-
-
-
?
trans,cis-3-hydroxymuconate + NADPH
-
390700
Cupriavidus necator
? + NADP+
-
-
-
?
trans,cis-3-hydroxymuconate + NADPH
-
390700
Cupriavidus necator JMP 134-1
? + NADP+
-
-
-
?
trans,cis-3-hydroxymuconate + NADPH
-
390700
Cupriavidus necator JMP 134(pJP4)
? + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 35000, SDS-PAGE
Cupriavidus necator
dimer
2 * 40000, SDS-PAGE
Cutaneotrichosporon cutaneum
dimer
-
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7.8
-
Cutaneotrichosporon cutaneum
7.5
-
-
Cupriavidus necator
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
8.5
-
Cupriavidus necator
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
8.5
-
Cupriavidus necator
Other publictions for EC 1.3.1.32
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740339
Kumar
Microbial degradation of 2,4-d ...
Cupriavidus necator, Cupriavidus necator JMP 134-1
Crit. Rev. Microbiol.
42
194-208
2016
-
-
-
-
-
-
-
-
-
-
-
4
-
30
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741383
Fujii
The crystal structure of maley ...
Rhizobium sp.
Proteins
84
1029-1042
2016
-
-
-
1
3
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
4
-
2
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
1
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
735508
Kasai
gamma-Resorcylate catabolic-pa ...
Rhodococcus jostii
Appl. Environ. Microbiol.
81
7656-7665
2015
-
-
1
-
-
-
-
-
-
-
1
2
-
3
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740533
Swain
-
In silico approach in the pred ...
Pseudomonas aeruginosa
Int. J. Bioautomat.
17
217-226
2013
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719179
Zhang
Identification of the para-nit ...
Pseudomonas sp. 1-7, Pseudomonas sp.
BMC Microbiol.
12
27
2012
-
-
1
-
-
-
-
-
-
14
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
1
1
-
1
1
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
2
-
1
1
1
-
1
1
1
-
-
-
-
-
-
-
724063
Kolvenbach
An unexpected gene cluster for ...
Sphingomonas sp., Sphingomonas sp. TTNP3
Appl. Microbiol. Biotechnol.
93
1315-1324
2012
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
724600
Hatta
Analysis of two gene clusters ...
Ralstonia pickettii
Biosci. Biotechnol. Biochem.
76
892-899
2012
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
724865
Chauhan
p-Nitrophenol degradation via ...
Ralstonia sp., Ralstonia sp. SJ98
Environ. Sci. Technol.
44
3435-3441
2010
-
-
1
-
-
1
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
3
2
1
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
697528
Chen
Cloning, overexpression, purif ...
Sphingobium chlorophenolicum
Curr. Microbiol.
58
599-603
2009
-
1
1
-
-
-
-
1
-
-
1
2
-
3
-
-
1
-
-
-
-
-
2
-
-
-
-
1
1
-
2
1
-
-
-
-
1
1
1
-
-
-
-
-
-
1
-
-
1
2
-
-
-
1
-
-
-
-
2
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
700063
Perez-Pantoja
Genuine genetic redundancy in ...
Cupriavidus necator, Cupriavidus necator JMP 134-1
Microbiology
155
3641-3651
2009
2
-
1
-
-
-
-
-
-
-
-
2
-
28
-
-
-
-
-
-
4
-
2
-
-
-
-
-
1
-
-
1
-
-
-
2
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
710729
Chauhan
Expression, purification, crys ...
Ralstonia sp., Ralstonia sp. SJ98
Acta Crystallogr. Sect. F
65
1313-1316
2009
-
-
1
1
-
-
-
-
-
-
1
-
-
6
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684201
Fujii
Crystallization and preliminar ...
Rhizobium sp.
Acta Crystallogr. Sect. F
64
737-739
2008
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
692101
Blasco
Pseudomonas aeruginosa strain ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa RW41
Environ. Microbiol.
10
1591-1600
2008
1
-
-
-
-
-
-
-
-
-
-
4
-
4
-
-
1
-
-
1
1
-
6
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
1
-
1
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674317
Yoshida
Biochemical and genetic analys ...
Rhizobium sp.
J. Bacteriol.
189
1573-1581
2006
-
1
1
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656738
Seibert
Characterization of a gene clu ...
Cupriavidus necator, Cupriavidus necator DSM 531
Microbiology
150
463-472
2004
-
-
1
-
-
-
-
-
-
-
-
2
-
6
-
-
1
-
-
1
1
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
1
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390706
Travkin
Purification and characterizat ...
Pimelobacter simplex, Pimelobacter simplex 3E
Biochemistry (Moscow)
64
625-630
1999
-
-
-
-
-
1
-
1
-
-
1
2
-
4
-
-
1
-
-
-
-
-
8
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
2
-
-
-
1
-
-
-
-
8
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
390705
Seibert
Characterization of the maleyl ...
Burkholderia cepacia, Burkholderia cepacia AC1100, Cupriavidus necator, Cupriavidus necator DSM 531, Cupriavidus necator JMP 134(pJP4), Cupriavidus necator JMP289, Pseudomonas aeruginosa, Pseudomonas aeruginosa RHO1, Pseudomonas fluorescens, Pseudomonas fluorescens ENV2030, Pseudomonas putida, Pseudomonas putida AC866(pAC27), Pseudomonas sp., Pseudomonas sp. B13, Rhodococcus opacus 1CP, Rhodococcus opacus
J. Bacteriol.
180
3503-3508
1998
-
-
3
-
-
-
-
-
-
-
1
16
-
54
-
-
2
-
-
-
-
-
50
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
16
-
-
-
2
-
-
-
-
50
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390704
Kasberg
Cloning, characterization, and ...
Burkholderia cepacia, Burkholderia cepacia AC1100, Cupriavidus necator, Cupriavidus necator JMP 134(pJP4), Cutaneotrichosporon cutaneum, Pseudomonas sp., Pseudomonas sp. B13
J. Bacteriol.
179
3801-3803
1997
-
-
2
-
-
-
-
-
-
-
-
7
-
35
-
-
3
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
3
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390702
Daubaras
Purification of hydroxyquinol ...
Burkholderia cepacia, Burkholderia cepacia AC1100, Pseudomonas sp., Pseudomonas sp. B13
Appl. Environ. Microbiol.
62
4276-4279
1996
-
-
1
-
-
-
-
-
-
-
-
4
-
27
-
-
1
-
-
-
-
-
8
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390703
Mueller
Maleylacetate reductases in ch ...
Cupriavidus necator, Cupriavidus necator JMP 134-1, Pseudomonas aeruginosa, Pseudomonas aeruginosa RHO1, Pseudomonas sp.
J. Bacteriol.
178
298-300
1996
-
-
-
-
-
-
-
24
-
-
3
3
-
31
-
-
2
-
-
-
1
-
57
3
1
-
-
-
1
-
1
2
-
-
-
-
-
-
2
-
-
-
-
-
-
24
-
-
3
3
-
-
-
2
-
-
1
-
57
3
1
-
-
-
1
-
1
-
-
-
-
-
-
-
390701
Kaschabek
Maleylacetate reductase of Pse ...
Cupriavidus necator, Cupriavidus necator JMP 134-1, Cutaneotrichosporon cutaneum, Pseudomonas sp., Pseudomonas sp. B13
J. Bacteriol.
177
320-325
1995
-
-
-
-
-
-
-
17
-
-
1
3
-
49
-
-
-
-
-
-
-
-
50
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
17
-
-
1
3
-
-
-
-
-
-
-
-
50
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390699
Kaschabek
Degradation of chloroaromatics ...
Cupriavidus necator, Cupriavidus necator JMP 134-1, Cutaneotrichosporon cutaneum, Delftia acidovorans, Delftia acidovorans CA28, Pseudomonas aeruginosa, Pseudomonas aeruginosa RHO1, Pseudomonas sp., Pseudomonas sp. B13, Pseudomonas sp. JS100, Pseudomonas sp. JS6
J. Bacteriol.
175
6075-6081
1993
-
-
1
-
-
-
12
5
-
-
4
6
-
59
-
-
1
-
-
-
1
1
49
4
1
1
-
1
1
-
-
6
-
-
-
-
-
1
6
-
-
-
-
12
-
5
-
-
4
6
-
-
-
1
-
-
1
1
49
4
1
1
-
1
1
-
-
-
-
-
-
-
-
-
390700
Seibert
Purification and characterizat ...
Cupriavidus necator, Cupriavidus necator JMP 134-1, Cupriavidus necator JMP 134(pJP4), Cutaneotrichosporon cutaneum, Pseudomonas sp., Pseudomonas sp. B13, Pseudomonas sp. PKO1, Sporotrichum pulverulentum
J. Bacteriol.
175
6745-6754
1993
1
-
1
-
-
1
6
8
-
-
5
8
-
55
-
-
3
-
-
-
1
2
25
3
-
-
-
-
2
1
1
7
1
-
-
1
-
1
7
-
-
1
-
6
1
8
-
-
5
8
-
-
-
3
-
-
1
2
25
3
-
-
-
-
2
1
1
-
-
-
-
-
-
-
390698
Engesser
-
3-fluorobenzoate enriched bact ...
Bacteria, Bacteria FLB 300, Cupriavidus necator, Cupriavidus necator JMP 134-1
Arch. Microbiol.
153
193-199
1990
-
-
-
-
-
-
-
-
-
-
-
4
-
28
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390696
Kukor
Recruitment of a chromosomally ...
Cupriavidus necator, Cupriavidus necator AEO106, Cupriavidus necator JMP 134-1, Cupriavidus necator JMP 134(pJP4), Cutaneotrichosporon cutaneum, Pseudomonas sp., Pseudomonas sp. B13, Pseudomonas sp. PKO1
J. Bacteriol.
171
3385-3390
1989
-
-
1
-
-
-
-
-
-
-
-
8
-
58
-
-
-
-
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390697
Pieper
-
Regulation of catabolic pathwa ...
Cupriavidus necator, Cupriavidus necator JMP 134-1
Arch. Microbiol.
151
365-371
1989
-
-
-
-
-
-
-
-
-
-
-
2
-
26
-
-
-
-
-
-
6
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
6
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390694
Oltmanns
-
Degradation of 1,4-dichloroben ...
Alcaligenes sp., Alcaligenes sp. R3, Pseudomonas putida, Pseudomonas putida WR1323, Pseudomonas sp., Pseudomonas sp. B13
Appl. Microbiol. Biotechnol.
28
609-616
1988
-
-
-
-
-
-
-
-
-
-
-
7
-
24
-
-
-
-
-
-
4
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
4
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390695
Pieper
-
Metabolism of 2,4-dichlorophen ...
Cupriavidus necator, Cupriavidus necator JMP 134-1
Arch. Microbiol.
150
95-102
1988
-
-
-
-
-
-
-
-
-
-
-
2
-
26
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390693
Reineke
Microbial metabolism of haloar ...
chlorobenzene-degrading bacterium strain WR1306, Cutaneotrichosporon cutaneum, Pseudomonas putida, Pseudomonas sp., Pseudomonas sp. B13
Appl. Environ. Microbiol.
47
395-402
1984
-
-
-
-
-
-
-
-
-
-
-
5
-
23
-
-
-
-
-
-
1
-
10
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
1
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390692
Shailubhai
Metabolism of resorcinol and s ...
Aspergillus niger, Cutaneotrichosporon cutaneum, Pseudomonas putida
Experientia
39
70-72
1983
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390691
Gaal
Induction of phenol-metabolizi ...
Cutaneotrichosporon cutaneum
Arch. Microbiol.
130
54-58
1981
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390690
Gaal
Maleylacetate reductase from T ...
Cutaneotrichosporon cutaneum, Pseudomonas putida, Sporotrichum pulverulentum
Biochem. J.
185
783-786
1980
-
-
-
-
-
-
3
-
-
-
2
3
-
4
-
-
1
-
-
-
1
2
6
1
-
-
-
-
1
-
-
4
-
-
-
-
-
-
4
-
-
-
-
3
-
-
-
-
2
3
-
-
-
1
-
-
1
2
6
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
390688
Gaal
Metabolism of phenol and resor ...
Cutaneotrichosporon cutaneum
J. Bacteriol.
137
13-21
1979
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390689
Sparnins
Catabolism of L-tyrosine in Tr ...
Cutaneotrichosporon cutaneum
J. Bacteriol.
138
425-430
1979
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439424
Buswell
-
Aromatic ring cleavage by the ...
Cutaneotrichosporon cutaneum, Pseudomonas putida, Sporotrichum pulverulentum
FEBS Lett.
104
258-260
1979
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-