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Literature summary for 1.3.1.32 extracted from
- Degradation of chloroaromatics: purification and characterization of maleylacetate reductase from Pseudomonas sp. strain B13 (1993), J. Bacteriol., 175, 6075-6081.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Pseudomonas sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Pseudomonas sp. |  |
| AgNO3 | - |
Pseudomonas sp. | |
| Cu2+ | - |
Pseudomonas sp. | |
| CuSO4 | - |
Cutaneotrichosporon cutaneum | |
| CuSO4 | - |
Pseudomonas sp. | |
| Hg2+ | - |
Pseudomonas sp. | |
| HgCl2 | - |
Pseudomonas sp. | |
| p-chloromercuribenzoate | - |
Cupriavidus necator | |
| p-chloromercuribenzoate | - |
Cutaneotrichosporon cutaneum | |
| p-chloromercuribenzoate | - |
Pseudomonas sp. | |
| Zn2+ | - |
Pseudomonas sp. | |
| ZnCl2 | - |
Pseudomonas sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
NADH | - |
Pseudomonas sp. | |
| 0.058 | - |
maleylacetate | - |
Pseudomonas sp. | |
| 0.076 | - |
2-chloromaleylacetate | - |
Pseudomonas sp. | |
| 0.077 | - |
NADPH | - |
Pseudomonas sp. | |
| 0.308 | - |
2-methylmaleylacetate | - |
Pseudomonas sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
2 * 37000, SDS-PAGE | Pseudomonas sp. |
| 74000 | - |
- |
Pseudomonas aeruginosa |
| 74000 | - |
- |
Cutaneotrichosporon cutaneum |
| 74000 | - |
gel filtration | Pseudomonas sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-maleylacetate + NADPH | Pseudomonas aeruginosa | - |
3-oxoadipate + NADP+ | - |
ir | |
| 2-maleylacetate + NADPH | Pseudomonas sp. | - |
3-oxoadipate + NADP+ | - |
ir | |
| 2-maleylacetate + NADPH | Cupriavidus necator | - |
3-oxoadipate + NADP+ | - |
ir | |
| 2-maleylacetate + NADPH | Delftia acidovorans | - |
3-oxoadipate + NADP+ | - |
ir | |
| 2-maleylacetate + NADPH | Cutaneotrichosporon cutaneum | - |
3-oxoadipate + NADP+ | - |
ir | |
| 2-maleylacetate + NADPH | Delftia acidovorans CA28 | - |
3-oxoadipate + NADP+ | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cupriavidus necator | - |
JMP 134 | - |
| Cupriavidus necator JMP 134-1 | - |
JMP 134 | - |
| Cutaneotrichosporon cutaneum | - |
- |
- |
| Delftia acidovorans | - |
CA28 | - |
| Delftia acidovorans CA28 | - |
CA28 | - |
| Pseudomonas aeruginosa | - |
- |
- |
| Pseudomonas aeruginosa RHO1 | - |
- |
- |
| Pseudomonas sp. | - |
- |
- |
| Pseudomonas sp. B13 | - |
- |
- |
| Pseudomonas sp. JS100 | - |
- |
- |
| Pseudomonas sp. JS6 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 582.9 | - |
- |
Pseudomonas sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 100 mM Tris/HCl buffer, pH 7.0, containing 50% (vol/vol) glycerol, 1 mM dithioerythritol and 0.5 mM EDTA, purified enzyme retains 80% of its initial activity after storage for 2 months | Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-chloromaleylacetate + NADH | - |
Pseudomonas aeruginosa | 2-maleylacetate + Cl- + NAD+ | - |
? | |
| 2-chloromaleylacetate + NADH | - |
Pseudomonas sp. | 2-maleylacetate + Cl- + NAD+ | - |
? | |
| 2-chloromaleylacetate + NADH | - |
Cupriavidus necator | 2-maleylacetate + Cl- + NAD+ | - |
? | |
| 2-chloromaleylacetate + NADH | - |
Pseudomonas sp. B13 | 2-maleylacetate + Cl- + NAD+ | - |
? | |
| 2-chloromaleylacetate + NADH | - |
Pseudomonas sp. JS100 | 2-maleylacetate + Cl- + NAD+ | - |
? | |
| 2-chloromaleylacetate + NADH | - |
Pseudomonas aeruginosa RHO1 | 2-maleylacetate + Cl- + NAD+ | - |
? | |
| 2-chloromaleylacetate + NADH | - |
Pseudomonas sp. JS6 | 2-maleylacetate + Cl- + NAD+ | - |
? | |
| 2-chloromaleylacetate + NADH | - |
Cupriavidus necator JMP 134-1 | 2-maleylacetate + Cl- + NAD+ | - |
? | |
| 2-maleylacetate + NAD(P)H | - |
Pseudomonas aeruginosa | 3-oxoadipate + NAD(P)+ | - |
ir | |
| 2-maleylacetate + NAD(P)H | - |
Pseudomonas sp. | 3-oxoadipate + NAD(P)+ | - |
ir | |
| 2-maleylacetate + NAD(P)H | - |
Cupriavidus necator | 3-oxoadipate + NAD(P)+ | - |
ir | |
| 2-maleylacetate + NAD(P)H | - |
Delftia acidovorans | 3-oxoadipate + NAD(P)+ | - |
ir | |
| 2-maleylacetate + NAD(P)H | - |
Cutaneotrichosporon cutaneum | 3-oxoadipate + NAD(P)+ | - |
ir | |
| 2-maleylacetate + NAD(P)H | - |
Pseudomonas sp. B13 | 3-oxoadipate + NAD(P)+ | - |
ir | |
| 2-maleylacetate + NAD(P)H | - |
Delftia acidovorans CA28 | 3-oxoadipate + NAD(P)+ | - |
ir | |
| 2-maleylacetate + NAD(P)H | - |
Pseudomonas sp. JS100 | 3-oxoadipate + NAD(P)+ | - |
ir | |
| 2-maleylacetate + NAD(P)H | - |
Pseudomonas aeruginosa RHO1 | 3-oxoadipate + NAD(P)+ | - |
ir | |
| 2-maleylacetate + NAD(P)H | - |
Pseudomonas sp. JS6 | 3-oxoadipate + NAD(P)+ | - |
ir | |
| 2-maleylacetate + NAD(P)H | - |
Cupriavidus necator JMP 134-1 | 3-oxoadipate + NAD(P)+ | - |
ir | |
| 2-maleylacetate + NADPH | - |
Pseudomonas aeruginosa | 3-oxoadipate + NADP+ | - |
ir | |
| 2-maleylacetate + NADPH | - |
Pseudomonas sp. | 3-oxoadipate + NADP+ | - |
ir | |
| 2-maleylacetate + NADPH | - |
Cupriavidus necator | 3-oxoadipate + NADP+ | - |
ir | |
| 2-maleylacetate + NADPH | - |
Delftia acidovorans | 3-oxoadipate + NADP+ | - |
ir | |
| 2-maleylacetate + NADPH | - |
Cutaneotrichosporon cutaneum | 3-oxoadipate + NADP+ | - |
ir | |
| 2-maleylacetate + NADPH | - |
Delftia acidovorans CA28 | 3-oxoadipate + NADP+ | - |
ir | |
| 2-methylmaleylacetate + NAD(P)H | - |
Pseudomonas aeruginosa | 5-methyl-3-oxoadipate + NAD(P)+ | - |
? | |
| 2-methylmaleylacetate + NAD(P)H | - |
Pseudomonas sp. | 5-methyl-3-oxoadipate + NAD(P)+ | - |
? | |
| 2-methylmaleylacetate + NAD(P)H | - |
Cupriavidus necator | 5-methyl-3-oxoadipate + NAD(P)+ | - |
? | |
| 2-methylmaleylacetate + NAD(P)H | - |
Pseudomonas sp. B13 | 5-methyl-3-oxoadipate + NAD(P)+ | - |
? | |
| 2-methylmaleylacetate + NAD(P)H | - |
Pseudomonas sp. JS100 | 5-methyl-3-oxoadipate + NAD(P)+ | - |
? | |
| 2-methylmaleylacetate + NAD(P)H | - |
Pseudomonas aeruginosa RHO1 | 5-methyl-3-oxoadipate + NAD(P)+ | - |
? | |
| 2-methylmaleylacetate + NAD(P)H | - |
Pseudomonas sp. JS6 | 5-methyl-3-oxoadipate + NAD(P)+ | - |
? | |
| 2-methylmaleylacetate + NAD(P)H | - |
Cupriavidus necator JMP 134-1 | 5-methyl-3-oxoadipate + NAD(P)+ | - |
? | |
| 3-methylmaleylacetate + NAD(P)H | - |
Pseudomonas aeruginosa | 4-methyl-3-oxoadipate + NAD(P)+ | - |
? | |
| 3-methylmaleylacetate + NAD(P)H | - |
Pseudomonas sp. | 4-methyl-3-oxoadipate + NAD(P)+ | - |
? | |
| 3-methylmaleylacetate + NAD(P)H | - |
Cupriavidus necator | 4-methyl-3-oxoadipate + NAD(P)+ | - |
? | |
| 3-methylmaleylacetate + NAD(P)H | - |
Pseudomonas sp. B13 | 4-methyl-3-oxoadipate + NAD(P)+ | - |
? | |
| 3-methylmaleylacetate + NAD(P)H | - |
Pseudomonas sp. JS100 | 4-methyl-3-oxoadipate + NAD(P)+ | - |
? | |
| 3-methylmaleylacetate + NAD(P)H | - |
Pseudomonas aeruginosa RHO1 | 4-methyl-3-oxoadipate + NAD(P)+ | - |
? | |
| 3-methylmaleylacetate + NAD(P)H | - |
Pseudomonas sp. JS6 | 4-methyl-3-oxoadipate + NAD(P)+ | - |
? | |
| 3-methylmaleylacetate + NAD(P)H | - |
Cupriavidus necator JMP 134-1 | 4-methyl-3-oxoadipate + NAD(P)+ | - |
? | |
| 5-methylmaleylacetate + NAD(P)H | - |
Pseudomonas aeruginosa | 2-methyl-3-oxoadipate + NADP+ | - |
? | |
| 5-methylmaleylacetate + NAD(P)H | - |
Pseudomonas sp. | 2-methyl-3-oxoadipate + NADP+ | - |
? | |
| 5-methylmaleylacetate + NAD(P)H | - |
Cupriavidus necator | 2-methyl-3-oxoadipate + NADP+ | - |
? | |
| 5-methylmaleylacetate + NAD(P)H | - |
Pseudomonas sp. B13 | 2-methyl-3-oxoadipate + NADP+ | - |
? | |
| 5-methylmaleylacetate + NAD(P)H | - |
Pseudomonas sp. JS100 | 2-methyl-3-oxoadipate + NADP+ | - |
? | |
| 5-methylmaleylacetate + NAD(P)H | - |
Pseudomonas aeruginosa RHO1 | 2-methyl-3-oxoadipate + NADP+ | - |
? | |
| 5-methylmaleylacetate + NAD(P)H | - |
Pseudomonas sp. JS6 | 2-methyl-3-oxoadipate + NADP+ | - |
? | |
| 5-methylmaleylacetate + NAD(P)H | - |
Cupriavidus necator JMP 134-1 | 2-methyl-3-oxoadipate + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Pseudomonas aeruginosa |
| dimer | - |
Cupriavidus necator |
| dimer | - |
Cutaneotrichosporon cutaneum |
| dimer | 2 * 37000, SDS-PAGE | Pseudomonas sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Pseudomonas sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 60 | - |
Pseudomonas sp. |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 13.9 | - |
maleylacetate | - |
Pseudomonas sp. | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
- |
Pseudomonas sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Pseudomonas sp. | |
| NADH | - |
Cupriavidus necator | |
| NADH | - |
Cutaneotrichosporon cutaneum | |
| NADPH | - |
Pseudomonas sp. | |
| NADPH | - |
Cupriavidus necator | |
| NADPH | - |
Cutaneotrichosporon cutaneum | |
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