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Literature summary for 1.3.1.3 extracted from

  • Li, Y.; Pan, H.; Chang, Y.; Dong, N.; Zou, L.; Liang, P.; Tian, W.; Chang, Z.
    Identification of key sites determining the cofactor specificity and improvement of catalytic activity of a steroid 5beta-reductase from Capsella rubella (2020), Enzyme Microb. Technol., 134, 109483 .
    View publication on PubMed
Search for more literature for 1.3.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Capsella rubella

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure Capsella rubella

Protein Variants

Protein Variants Comment Organism
R63K mutant is able to use NADH as cofactor Capsella rubella
R63K/R64H increases enzymatic activity by 13.8fold with NADH as cofactor Capsella rubella
R64G mutant exhibits significantly improved activity with NADH Capsella rubella
R64H mutant exhibits significantly improved activity with NADH Capsella rubella
R64S mutant exhibits significantly improved activity with NADH Capsella rubella
R64T mutant exhibits significantly improved activity with NADH Capsella rubella

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.358
-
 2-cyclohexen-1-one mutant R63K/R64H, cofactor NADPH, pH 6, 40°C Capsella rubella
0.443
-
 2-cyclohexen-1-one wild-type, cofactor NADPH, pH 6, 40°C Capsella rubella
0.541
-
 2-cyclohexen-1-one mutant R63K/R64H, cofactor NADH, pH 6, 40°C Capsella rubella

Organism

Organism UniProt Comment Textmining
Capsella rubella R0F4W3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(20S)-21-hydroxy-20-methylpregn-4-en-3-one + NADPH + H+
-
 Capsella rubella ? + NADP+
-
 ?
2-cyclohexen-1-one + NADPH + H+
-
 Capsella rubella ? + NADP+
-
 ?
4-androstene-3,17-dione + NADPH + H+
-
 Capsella rubella 5beta-androstan-3,17-dione + NADP+
-
 ?
ethyl acrylate + NADPH + H+
-
 Capsella rubella ? + NADP+
-
 ?
progesterone + NADPH + H+
-
 Capsella rubella 5beta-pregnane-3,20-dione + NADP+
-
 ?
testosterone + NADPH + H+
-
 Capsella rubella (5beta,17beta)-17-hydroxyandrostan-3-one + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
CARUB_v10005016mg
-
 Capsella rubella
St5betaR1
-
 Capsella rubella

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.23
-
 2-cyclohexen-1-one wild-type, cofactor NADPH, pH 6, 40°C Capsella rubella
0.28
-
 2-cyclohexen-1-one mutant R63K/R64H, cofactor NADPH, pH 6, 40°C Capsella rubella
0.81
-
 2-cyclohexen-1-one mutant R63K/R64H, cofactor NADH, pH 6, 40°C Capsella rubella

Cofactor

Cofactor Comment Organism Structure
NADPH wild-type is strictly dependen on NADPH Capsella rubella

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.52
-
 2-cyclohexen-1-one wild-type, cofactor NADPH, pH 6, 40°C Capsella rubella
0.611
-
 2-cyclohexen-1-one mutant R63K/R64H, cofactor NADPH, pH 6, 40°C Capsella rubella
1.5
-
 2-cyclohexen-1-one mutant R63K/R64H, cofactor NADH, pH 6, 40°C Capsella rubella