Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain C41(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, in 0.1 M Tris/HEPES (pH 7.5), 10-20% (w/v) polyethylene glycol 4000, and 10% isopropyl alcohol | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E120A | mutant is devoid of activity | Homo sapiens |
Y58F | mutant is devoid of activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
testosterone | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
testosterone | in 100 mM potassium phosphate buffer (pH 6.0), at 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-Sepharose column chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cortisone + NADPH + H+ | - |
Homo sapiens | 5beta-17,21dihydroxy-pregnan-3,11,20-trione + NADP+ | - |
? | |
progesterone + NADPH + H+ | - |
Homo sapiens | 5beta-pregnane-3,20-dione + NADP+ | - |
? | |
testosterone + NADPH + H+ | - |
Homo sapiens | (5beta,17beta)-17-hydroxyandrostan-3-one + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AKR1D1 | - |
Homo sapiens |
DELTA4-3-ketosteroid 5beta-reductase | - |
Homo sapiens |
steroid 5beta-reductase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0145 | - |
testosterone | in 100 mM potassium phosphate buffer (pH 6.0), at 37°C | Homo sapiens |