Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method in 20% (w/v) PEG-4K, 0.1 M sodium cacodylate (pH 6.5), 0.2 M sodium citrate, 0.5 M (NH4)2SO4 | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
recombinant GST-5beta-reductase fusion protein, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P51857 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose column chromatography and Sephacryl-S100 gel filtration | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
17,21-dihydroxy-5beta-pregnane-3,11,20-trione + NADP+ = cortisone + NADPH + H+ | catalyzes the reduction of the DELTA4 double bond of DELTA4-3-ketosteroids, converting those steroids into 5beta-dihydro-3-ketosteroids | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7alpha,12beta-dihydroxy-4-cholesten-3-one + NADPH + H+ | - |
Homo sapiens | (5beta,7alpha,12beta)-7,12-dihydroxycholestan-3-one + NADP+ | - |
? | |
7alpha-hydroxy-4-cholesten-3-one + NADPH | - |
Homo sapiens | (5beta,7alpha)-7-hydroxycholestan-3-one + NADP+ | - |
? | |
progesterone + NADPH + H+ | - |
Homo sapiens | pregnan-3,20-dione + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5beta-red | - |
Homo sapiens |
DELTA4-3-ketosteroid 5beta-reductase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |