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Literature summary for 1.3.1.27 extracted from

  • Porte, S.; Moeini, A.; Reche, I.; Shafqat, N.; Oppermann, U.; Farres, J.; Pares, X.
    Kinetic and structural evidence of the alkenal/one reductase specificity of human zeta-crystallin (2011), Cell. Mol. Life Sci., 68, 1065-1077.
    View publication on PubMed
Search for more literature for 1.3.1.27

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
to 1.85 A resolution. The overall structure displays the typical medium-chain dehydrogenase fold with two domains: the catalytic domain, residues 1-128 and 271-329, and the coenzyme-binding domain, residues 129-270, which are separated by a deep cleft that binds the cofactor NADP+ and the substrate Homo sapiens

Protein Variants

Protein Variants Comment Organism
Y53F substrate-pocket residue, large increase in Km values for all substrates and the cofactor, but mainly towards 3-buten-2-one and propenal with a 30fold increase Homo sapiens
Y59F substrate-pocket residue, almost the same kinetic parameter values as the wild-type enzyme for 2-alkenals Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 NADPH wild-type, pH 7.5, temperature not specified in the publication Homo sapiens
0.015
-
 NADPH mutant Y59F, pH 7.5, temperature not specified in the publication Homo sapiens
0.035
-
 3-buten-2-one wild-type, pH 7.5, temperature not specified in the publication Homo sapiens
0.06
-
 NADPH mutant Y53F, pH 7.5, temperature not specified in the publication Homo sapiens
0.13
-
 4-hydroxy-2-hexenal wild-type, pH 7.5, temperature not specified in the publication Homo sapiens
0.48
-
 2-nonenal wild-type, pH 7.5, temperature not specified in the publication Homo sapiens
0.55
-
 4-hydroxy-2-nonenal wild-type, pH 7.5, temperature not specified in the publication Homo sapiens
1.26
-
 4-hydroxy-2-hexenal mutant Y59F, pH 7.5, temperature not specified in the publication Homo sapiens
3.67
-
 4-hydroxy-2-hexenal mutant Y53F, pH 7.5, temperature not specified in the publication Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q08257
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nonenal + NADPH + H+
-
 Homo sapiens nonanal + NADP+
-
 ir
3-buten-2-one + NADPH + H+
-
 Homo sapiens butan-2-one + NADP+
-
 ?
4-hydroxy-2-hexenal + NADPH + H+
-
 Homo sapiens 4-hydroxyhexadecanal + NADP+
-
 ?
4-hydroxy-2-nonenal + NADPH + H+
-
 Homo sapiens 4-hydroxynonanal + NADP+
-
 ?
additional information enzyme additionally catalyzes the reduction of quinones, reaction of EC 1.6.5.5. The enzyme shows a low level of activity with cinnamaldehyde and no activity with 2-cyclohexen-1-one and 15-ketoprostaglandin E2. No activity is detected with nonanal and 2-nonene Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
zeta-Crystallin
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.113
-
 2-nonenal wild-type, pH 7.5, temperature not specified in the publication Homo sapiens
0.167
-
 3-buten-2-one wild-type, pH 7.5, temperature not specified in the publication Homo sapiens
1.15
-
 4-hydroxy-2-nonenal wild-type, pH 7.5, temperature not specified in the publication Homo sapiens
1.33
-
 4-hydroxy-2-hexenal wild-type, pH 7.5, temperature not specified in the publication Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.02
-
 4-hydroxy-2-hexenal mutant Y53F, pH 7.5, temperature not specified in the publication Homo sapiens
0.23
-
 2-nonenal wild-type, pH 7.5, temperature not specified in the publication Homo sapiens
1.67
-
 4-hydroxy-2-hexenal mutant Y59F, pH 7.5, temperature not specified in the publication Homo sapiens
2.1
-
 4-hydroxy-2-nonenal wild-type, pH 7.5, temperature not specified in the publication Homo sapiens
4.8
-
 3-buten-2-one wild-type, pH 7.5, temperature not specified in the publication Homo sapiens
10.2
-
 4-hydroxy-2-hexenal wild-type, pH 7.5, temperature not specified in the publication Homo sapiens