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Literature summary for 1.3.1.24 extracted from

  • Chu, W.T.; Nesbitt, N.M.; Gnatenko, D.V.; Li, Z.; Zhang, B.; Seeliger, M.A.; Browne, S.; Mantle, T.J.; Bahou, W.F.; Wang, J.
    Enzymatic activity and thermodynamic stability of biliverdin IXbeta reductase are maintained by an active site serine (2017), Chemistry, 23, 1891-1900.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.24

Protein Variants

Protein Variants Comment Organism
H132A the mutant exhibits an increased Km value for NADPH compared to the wild type enzyme Homo sapiens
R124A the mutant exhibits an increased Km value for NADPH compared to the wild type enzyme Homo sapiens
R174A the mutant exhibits an increased Km value for NADPH compared to the wild type enzyme Homo sapiens
R35A the mutant exhibits a strongly increased Km value for NADPH compared to the wild type enzyme Homo sapiens
R35S the mutant exhibits a strongly increased Km value for NADPH compared to the wild type enzyme Homo sapiens
R39A the mutant exhibits a reduced Km value for NADPH compared to the wild type enzyme Homo sapiens
R78A the mutant exhibits an increased Km value for NADPH compared to the wild type enzyme Homo sapiens
S111A the mutant shows strongly reduced activity compared to the wild type enzyme Homo sapiens
S111L the mutant shows strongly reduced activity compared to the wild type enzyme Homo sapiens
W116A the mutant exhibits a reduced Km value for NADPH compared to the wild type enzyme Homo sapiens
W116F the mutant exhibits a reduced Km value for NADPH compared to the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
 NADPH mutant enzyme W116F, at pH 7.2 and 30°C Homo sapiens
0.0016
-
 NADPH mutant enzyme R39A, at pH 7.2 and 30°C Homo sapiens
0.0017
-
 NADPH mutant enzyme W116A, at pH 7.2 and 30°C Homo sapiens
0.0024
-
 NADPH wild type enzyme, at pH 7.2 and 30°C Homo sapiens
0.003
-
 NADPH mutant enzyme R174A, at pH 7.2 and 30°C Homo sapiens
0.0045
-
 NADPH mutant enzyme R124A, at pH 7.2 and 30°C Homo sapiens
0.0047
-
 NADPH mutant enzyme R78A, at pH 7.2 and 30°C Homo sapiens
0.0049
-
 NADPH mutant enzyme H132A, at pH 7.2 and 30°C Homo sapiens
0.0058
-
 NADPH mutant enzyme S111L, at pH 7.2 and 25°C Homo sapiens
0.0067
-
 NADPH mutant enzyme S111A, at pH 7.2 and 25°C Homo sapiens
0.0083
-
 NADPH wild type enzyme, at pH 7.2 and 25°C Homo sapiens
0.029
-
 NADPH mutant enzyme R35S, at pH 7.2 and 30°C Homo sapiens
0.059
-
 NADPH mutant enzyme R35A, at pH 7.2 and 30°C Homo sapiens
0.107
-
 NADH mutant enzyme R35S, at pH 7.2 and 30°C Homo sapiens
0.108
-
 NADH mutant enzyme R35A, at pH 7.2 and 30°C Homo sapiens
0.118
-
 NADH wild type enzyme, at pH 7.2 and 30°C Homo sapiens
0.15
-
 NADH mutant enzyme R78A, at pH 7.2 and 30°C Homo sapiens
0.186
-
 NADH mutant enzyme W116A, at pH 7.2 and 30°C Homo sapiens
0.305
-
 NADH mutant enzyme R39A, at pH 7.2 and 30°C Homo sapiens
0.319
-
 NADH mutant enzyme W116F, at pH 7.2 and 30°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
biliverdin IXbeta + NADH + H+ Homo sapiens
-
 bilirubin + NAD+
-
 r
biliverdin IXbeta + NADPH + H+ Homo sapiens
-
 bilirubin + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens P30043
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biliverdin IXbeta + NADH + H+
-
 Homo sapiens bilirubin + NAD+
-
 r
biliverdin IXbeta + NADPH + H+
-
 Homo sapiens bilirubin + NADP+
-
 r

Synonyms

Synonyms Comment Organism
biliverdin IXbeta reductase
-
 Homo sapiens
BLVRB
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 NADPH mutant enzyme S111L, at pH 7.2 and 25°C Homo sapiens
0.025
-
 NADPH mutant enzyme S111A, at pH 7.2 and 25°C Homo sapiens
0.097
-
 NADPH mutant enzyme R124A, at pH 7.2 and 30°C Homo sapiens
0.171
-
 NADPH wild type enzyme, at pH 7.2 and 25°C Homo sapiens
0.237
-
 NADPH mutant enzyme H132A, at pH 7.2 and 30°C Homo sapiens
0.237
-
 NADPH wild type enzyme, at pH 7.2 and 30°C Homo sapiens
0.4
-
 NADPH mutant enzyme R174A, at pH 7.2 and 30°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Homo sapiens
NADPH
-
 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.7
-
 NADPH mutant enzyme S111L, at pH 7.2 and 25°C Homo sapiens
3.7
-
 NADPH mutant enzyme S111A, at pH 7.2 and 25°C Homo sapiens
21
-
 NADPH wild type enzyme, at pH 7.2 and 25°C Homo sapiens