Cloned (Comment) | Organism |
---|---|
biliverdin reductase cDNA cloned into pUC18, used for expression and purification | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
C280A | although modification of either of the two cysteines located near the C-terminus significantly reduces activity with both cofactors, these mutations do not inactivate the enzyme, mutation of both C-terminal cysteines causes inactivation of the enzyme, comparison of Km values suggests that Cys 280 principally functions in substrate binding | Rattus norvegicus |
C291A | although modification of either of the two cysteines located near the C-terminus significantly reduces activity with both cofactors, these mutations do not inactivate the enzyme, mutation of both C-terminal cysteines causes inactivation of the enzyme, comparison of Km values suggests that Cys 291 is predominantly involved in cofactor binding. | Rattus norvegicus |
C73A | the modification of the amino-proximal cysteine, which is flanked by a tyrosine residue, completely inactivates the enzyme with NADH at pH 6.75 and NADPH at pH 8.7 | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
NADPH | in the presence of 0.005 mM biliverdin, biliverdin reductase C280A | Rattus norvegicus | |
0.0029 | - |
NADPH | in the presence of 0.005 mM biliverdin, biliverdin reductase wild-type | Rattus norvegicus | |
0.0032 | - |
Biliverdin | cosubstrate NADPH, biliverdin reductase C291A | Rattus norvegicus | |
0.0034 | - |
Biliverdin | cosubstrate NADPH, biliverdin reductase wild-type | Rattus norvegicus | |
0.0049 | - |
Biliverdin | cosubstrate NADH, biliverdin reductase wild-type | Rattus norvegicus | |
0.0052 | - |
Biliverdin | cosubstrate NADH, biliverdin reductase C291A | Rattus norvegicus | |
0.0097 | - |
Biliverdin | cosubstrate NADH, biliverdin reductase C280A | Rattus norvegicus | |
0.0102 | - |
Biliverdin | cosubstrate NADPH, biliverdin reductase C280A | Rattus norvegicus | |
0.0133 | - |
NADPH | in the presence of 0.005 mM biliverdin, biliverdin reductase C291A | Rattus norvegicus | |
0.333 | - |
NADH | in the presence of 0.005 mM biliverdin, biliverdin reductase wild-type | Rattus norvegicus | |
0.394 | - |
NADH | in the presence of 0.005 mM biliverdin, biliverdin reductase C280A | Rattus norvegicus | |
0.567 | - |
NADH | in the presence of 0.005 mM biliverdin, biliverdin reductase C291A | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of biliverdin reductase expressed in Escherichia coli and from rat tissue | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bilirubin + NAD+ | - |
Rattus norvegicus | biliverdin + NADH + H+ | - |
? |