Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.24 extracted from

  • Kutty, R.K.; Maines, M.D.
    Purification and characterization of biliverdin reductase from rat liver (1981), J. Biol. Chem., 256, 3956-3962.
    View publication on PubMed
Search for more literature for 1.3.1.24

General Stability

General Stability Organism
loss of activity in solutions with protein concentration below 0.030-0.035 mg/ml Rattus norvegicus
NADPH stabilizes Rattus norvegicus
repeated freeze-thawing: little loss of activity , no significant loss of activity, partially purified enzyme Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) pretreatment of the enzyme with NADPH and biliverdins fully protects Rattus norvegicus
bilirubin inhibits the enzyme in a competitive manner Rattus norvegicus
Biliverdin the enzyme is inhibited by the substrate when the concentration exceeds 0.004-0.005 mM Rattus norvegicus
Hematin
-
 Rattus norvegicus
HgCl2 irreversible inhibition Rattus norvegicus
iodoacetamide pretreatment of the enzyme with NADPH and biliverdins fully protects Rattus norvegicus
Metalloporphyrin complexes
-
 Rattus norvegicus
N-ethylmaleimide pretreatment of the enzyme with NADPH and biliverdins fully protects Rattus norvegicus
p-chloromercuribenzoate pretreatment of the enzyme with NADPH and biliverdins fully protects Rattus norvegicus
SH-reagents e.g. 5,5'-dithiobis(2-nitrobenzoic acid), N-ethylmaleimide, pretreatment of the enzyme with NADPH and biliverdins fully protects Rattus norvegicus
zinc-protoporphyrin
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 NADPH
-
 Rattus norvegicus
0.003
-
 Biliverdin cosubstrate NADPH Rattus norvegicus
0.005
-
 Biliverdin cosubstrate NADH Rattus norvegicus
0.27
-
 NADH
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Rattus norvegicus 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000 36000 liver Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, several months -25°C, 1 month: no loss of activity, 2 months: 50% loss of activity Rattus norvegicus
0-4°C, 5 days Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bilirubin + NAD+
-
 Rattus norvegicus biliverdin + NADH + H+
-
 ?
biliverdin + NAD(P)H
-
 Rattus norvegicus bilirubin + NAD(P)+
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 10 min, no loss of activity Rattus norvegicus
55
-
 10 min, complete loss of activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 biliverdin + NADH Rattus norvegicus
8.7
-
 biliverdin + NADPH Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.4
-
 0.1 M potassium phosphate buffer, most stable Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Rattus norvegicus
NADPH
-
 Rattus norvegicus