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Literature summary for 1.3.1.22 extracted from

  • Peng, H.M.; Valentin-Goyco, J.; Im, S.C.; Han, B.; Liu, J.; Qiao, J.; Auchus, R.J.
    Expression in Escherichia Coli, purification, and functional reconstitution of human steroid 5alpha-reductases (2020), Endocrinology, 161, bqaa117 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.22

Cloned(Commentary)

Cloned (Comment) Organism
expression of polyhistidine-tagged, codon-optimized cDNA in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
3-keto-5alpha-abiraterone
-
 Homo sapiens
3-keto-Delta4-abiraterone
-
 Homo sapiens
finasteride
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0001
-
 progesterone pH 6.0, 37°C Homo sapiens
0.0003
-
 progesterone pH 7.4, 37°C Homo sapiens
0.0008
-
 testosterone pH 6.0, 37°C Homo sapiens
0.0014
-
 androstenedione pH 6.0, 37°C Homo sapiens
0.003
-
 3-keto-Delta4-abiraterone pH 7.4, 37°C Homo sapiens
0.0031
-
 androstenedione pH 7.4, 37°C Homo sapiens
0.0061
-
 17alpha-hydroxyprogesterone pH 6.0, 37°C Homo sapiens
0.0088
-
 17alpha-hydroxyprogesterone pH 7.4, 37°C Homo sapiens
0.011
-
 NADPH pH 7.4, 37°C Homo sapiens
0.0156
-
 testosterone pH 7.4, 37°C Homo sapiens
0.016
-
 NADPH pH 6.0, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P18405 isoform SRD5A1
-
Homo sapiens P31213 isoform SRD5A2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, use of detergents Triton X-100 and Nonidet P-40 for solubilization Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
functional reconstitution of recombinant enzyme, incorporation of the purified isoenzymes into Triton X-100-saturated dioleoylphosphatidylcholine liposomes and removal of excess detergent with polystyrene beads Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
17alpha-hydroxyprogesterone + NADPH + H+
-
 Homo sapiens 17alpha-hydroxy-5alpha-pregnan-3,20-dione + NADP+
-
 ?
3-keto-delta4-abiraterone + NADPH + H+
-
 Homo sapiens ? + NADP+
-
 ?
androstenedione + NADPH + H+
-
 Homo sapiens 5alpha-androstan-3,17-dione + NADP+
-
 ?
progesterone + NADPH + H+
-
 Homo sapiens 5alpha-pregnan-3,20-dione + NADP+
-
 ?
testosterone + NADPH + H+
-
 Homo sapiens 5alpha-dihydrotestosterone + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 22000, SDS-PAGE, x * 29000, calculated from sequence Homo sapiens
? x * 22000, SDS-PAGE, x * 29000, calculated fromn sequence Homo sapiens

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor: NADH Homo sapiens