Literature summary for 1.3.1.22 extracted from

Wang, X.; Liao, J.; Yin, D.; Zhan, F.; Dai, S.; Xie, G.; Sang, X.
Establishment of a novel model for studying the effects of extracts of Chinese herb medicine on human type II 5alpha-reductase in vitro. (2010), Yakugaku Zasshi, 130, 1207-1214.

Search for more literature for 1.3.1.22

Cloned(Commentary)

Cloned (Comment)	Organism
gene SRD5A2, DNA and amino acid sequence determination, expression of His-tagged enzyme in Escherichia coli strain DH5alpha and in CHO cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
2,6,4'-methoxybenzophenone	from Anemarrhena aspholoides rhizomes	Homo sapiens
cis-hinokiresinol	from Anemarrhena aspholoides rhizomes	Homo sapiens
finasteride	-	Homo sapiens
mangiferin	a 1,3,6,7-tetrahydroxyxanthone-C2-beta-D-glucoside, from Anemarrhena aspholoides rhizomes, directly inhibits SRD5A2 binding to a complex of enzyme and substrate	Homo sapiens
additional information	inhibitory effects on SRD5A2 of Chinese medicine herb extracts, of Anemarrhena aspholoides rhizomes, Pellodendron amurense bark, and Cinnamomum cassia bark, collected in China, Korea, and Japan, overview	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000054	-	testosterone	pH 7.4, 37°C, recombinant His-tagged enzyme	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
testosterone + NADPH + H+	Homo sapiens	the enzyme performs conversion of testosterone to dihydrotestosterone	5alpha-dihydrotestosterone + NADP+	-	r

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P31213	gene SRD5A2	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged SRD5A2 from CHO cells by nickel affinity chromatography and anion exchange chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
testosterone + NADPH + H+	-	Homo sapiens	5alpha-dihydrotestosterone + NADP+	-	r
testosterone + NADPH + H+	the enzyme performs conversion of testosterone to dihydrotestosterone	Homo sapiens	5alpha-dihydrotestosterone + NADP+	-	r

Synonyms

Synonyms	Comment	Organism
SRD5A2	-	Homo sapiens
steroid 5alpha-reductase type II	-	Homo sapiens
type II 5alpha-reductase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.000051	-	pH 7.4, 37°C, recombinant His-tagged enzyme	Homo sapiens	finasteride

General Information

General Information	Comment	Organism
physiological function	SRD5A2 and dihydrotestosterone play important roles in benign prostatic hyperplasia	Homo sapiens

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Release 2023.1 (January 2023)