Cloned (Comment) | Organism |
---|---|
gene SRD5A2, DNA and amino acid sequence determination, expression of His-tagged enzyme in Escherichia coli strain DH5alpha and in CHO cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,6,4'-methoxybenzophenone | from Anemarrhena aspholoides rhizomes | Homo sapiens | |
cis-hinokiresinol | from Anemarrhena aspholoides rhizomes | Homo sapiens | |
finasteride | - |
Homo sapiens | |
mangiferin | a 1,3,6,7-tetrahydroxyxanthone-C2-beta-D-glucoside, from Anemarrhena aspholoides rhizomes, directly inhibits SRD5A2 binding to a complex of enzyme and substrate | Homo sapiens | |
additional information | inhibitory effects on SRD5A2 of Chinese medicine herb extracts, of Anemarrhena aspholoides rhizomes, Pellodendron amurense bark, and Cinnamomum cassia bark, collected in China, Korea, and Japan, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000054 | - |
testosterone | pH 7.4, 37°C, recombinant His-tagged enzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
testosterone + NADPH + H+ | Homo sapiens | the enzyme performs conversion of testosterone to dihydrotestosterone | 5alpha-dihydrotestosterone + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P31213 | gene SRD5A2 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged SRD5A2 from CHO cells by nickel affinity chromatography and anion exchange chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
testosterone + NADPH + H+ | - |
Homo sapiens | 5alpha-dihydrotestosterone + NADP+ | - |
r | |
testosterone + NADPH + H+ | the enzyme performs conversion of testosterone to dihydrotestosterone | Homo sapiens | 5alpha-dihydrotestosterone + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
SRD5A2 | - |
Homo sapiens |
steroid 5alpha-reductase type II | - |
Homo sapiens |
type II 5alpha-reductase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000051 | - |
pH 7.4, 37°C, recombinant His-tagged enzyme | Homo sapiens | finasteride |
General Information | Comment | Organism |
---|---|---|
physiological function | SRD5A2 and dihydrotestosterone play important roles in benign prostatic hyperplasia | Homo sapiens |