Application | Comment | Organism |
---|---|---|
additional information | two isoenzymes of 5alphaR is probably characteristic of the whole plant kingdom | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
into vector pTarget and expressed in COS-7 cells | Arabidopsis thaliana |
into vector pTarget and expressed in COS-7 cells | Solanum lycopersicum |
Protein Variants | Comment | Organism |
---|---|---|
K204E | det2-1 mutant, substitution of lysine for Glu-204 totally inactivates the steroid 5alpha-reductase activity, residual activity for campestenone and progesterone | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-MA | active at 0.01 mM | Solanum lycopersicum | |
AFA131 | active at 0.01 mM | Solanum lycopersicum | |
AFA192 | active at 0.01 mM | Solanum lycopersicum | |
AFA27 | active at 0.01 mM | Solanum lycopersicum | |
AFA76 | active at 0.01 mM, most potent inhibitor among non-steroid compounds tested | Solanum lycopersicum | |
AS601811 | active at 0.01 mM | Solanum lycopersicum | |
finasteride | active at 0.01 mM | Solanum lycopersicum | |
PD17 | active at 0.01 mM | Solanum lycopersicum | |
PD91 | active at 0.01 mM | Solanum lycopersicum | |
VG106 | active at 0.01 mM | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00075 | - |
progesterone | - |
Solanum lycopersicum | |
0.02634 | - |
androstenedione | - |
Solanum lycopersicum | |
0.0446 | - |
testosterone | - |
Solanum lycopersicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Solanum lycopersicum | Q5K2N1 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
callus | - |
Solanum lycopersicum | - |
leaf | - |
Arabidopsis thaliana | - |
leaf | higher levels | Solanum lycopersicum | - |
root | - |
Solanum lycopersicum | - |
seed | - |
Arabidopsis thaliana | - |
seed | - |
Solanum lycopersicum | - |
stem | - |
Solanum lycopersicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
androstenedione + NADPH + H+ | - |
Solanum lycopersicum | 5alpha-androstan-3,17-dione + NADP+ | - |
? | |
campestenone + NADPH | reduction at very low levels | Solanum lycopersicum | 5alpha-campestan-3-one + NADP+ | - |
? | |
additional information | no activity for campestenone | Arabidopsis thaliana | ? | - |
? | |
progesterone + NADPH + H+ | - |
Arabidopsis thaliana | 5alpha-pregnan-3-20-dione + NADP+ | - |
? | |
progesterone + NADPH + H+ | - |
Solanum lycopersicum | 5alpha-pregnan-3-20-dione + NADP+ | - |
? | |
testosterone + NADPH + H+ | - |
Solanum lycopersicum | 5alpha-dihydrotestosterone + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5alphaR | - |
Arabidopsis thaliana |
5alphaR | - |
Solanum lycopersicum |
AtDET2 | - |
Arabidopsis thaliana |
LeDET2 | - |
Solanum lycopersicum |
steroid 5alpha-reductase | - |
Arabidopsis thaliana |
steroid 5alpha-reductase | - |
Solanum lycopersicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Solanum lycopersicum |