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Literature summary for 1.3.1.22 extracted from
- Characterization and localization of progesterone 5alpha-reductase from cell cultures of foxglove (Digitalis lanata EHRH) (1990), Biochem. J., 266, 41-46.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 3fold activity at 1 mM | Digitalis lanata |  |
| dithiothreitol | 3fold activity at 1 mM | Digitalis lanata | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Digitalis lanata | |
| Co2+ | - |
Digitalis lanata | |
| Zn2+ | - |
Digitalis lanata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
progesterone | - |
Digitalis lanata | |
| 0.13 | - |
NADPH | - |
Digitalis lanata | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Digitalis lanata | 5783 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | stimulatory at concentration 2 mM | Digitalis lanata | |
| Mg2+ | stimulatory at concentration 1 mM | Digitalis lanata | |
| additional information | not dependent on divalent cation | Digitalis lanata |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| progesterone + NADPH + H+ | Digitalis lanata | biosynthesis of cardenolides | 5alpha-pregnan-3-20-dione + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Digitalis lanata | - |
floxglove | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Digitalis lanata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.34 | - |
with NADPH | Digitalis lanata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| progesterone + NADPH + H+ | - |
Digitalis lanata | 5alpha-pregnan-3-20-dione + NADP+ | - |
? | |
| progesterone + NADPH + H+ | biosynthesis of cardenolides | Digitalis lanata | 5alpha-pregnan-3-20-dione + NADP+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Digitalis lanata |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Digitalis lanata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Digitalis lanata |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 7.5 | - |
Digitalis lanata |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | cannot be replaced by NADH | Digitalis lanata | |
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