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Literature summary for 1.3.1.20 extracted from

  • Carbone, V.; Endo, S.; Sumii, R.; Chung, R.P.; Matsunaga, T.; Hara, A.; El-Kabbani, O.
    Structures of dimeric dihydrodiol dehydrogenase apoenzyme and inhibitor complex: probing the subunit interface with site-directed mutagenesis (2007), Proteins Struct. Funct. Bioinform., 70, 176-187.
No PubMed abstract available
Search for more literature for 1.3.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Macaca fascicularis

Crystallization (Commentary)

Crystallization (Comment) Organism
vapour diffusion method with 2 M ammonium phosphate and 0.1 M sodium citrate buffer (pH 5.6) and 20% glycerol Macaca fascicularis

Protein Variants

Protein Variants Comment Organism
R148A activity and temperature stability are similar to the wild type enzyme Macaca fascicularis
R148A/R202A very low activity, the Vmax value of the mutant enzyme is 3.7fold lower than the wild type enzyme, displays significant temperature sensitivity Macaca fascicularis
R202A activity and temperature stability are similar to the wild type enzyme Macaca fascicularis

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxyacetophenone
-
 Macaca fascicularis

Organism

Organism UniProt Comment Textmining
Macaca fascicularis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Red Sepharose column chromatography, and Sephadex G-100 gel filtration Macaca fascicularis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-xylose + NADP+
-
 Macaca fascicularis D-xylono-1,5-lactone + NADPH + H+
-
 ?
trans-1,2-dihydrobenzene-1,2-diol + NAD(P)+
-
 Macaca fascicularis catechol + NAD(P)H
-
 ?

Subunits

Subunits Comment Organism
dimer X-ray crystallography, the intact dimer is not essential for catalytic activity Macaca fascicularis

Synonyms

Synonyms Comment Organism
dihydrodiol dehydrogenase
-
 Macaca fascicularis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 the wild type enzyme and mutant enzymes R148A and R202A are stable at 37°C Macaca fascicularis

Cofactor

Cofactor Comment Organism Structure
NADP+ dependent on Macaca fascicularis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00097
-
 wild type enzyme Macaca fascicularis 4-hydroxyacetophenone