Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Sulfobromophthalein | DD4 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
DD4 double mutant S145C/L311V overexpressed in Escherichia coli | Homo sapiens |
DD4, isolation of mRNA of native mutants isolated from a liver sample, synthesis of cDNA by RT-PCR, polymorphism of DD4 gene | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
L311V | native mutant with C to G replacement in DNA at position 931 | Homo sapiens |
S145C | native mutant with C to G replacement in DNA at position 434, no significant effect on activity | Homo sapiens |
S145C/L311V | constructed double mutant with 3 to 5fold decreased activity for xenobiotic and steroidal substrates | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Betamethasone | DD4 wild-type and mutants | Homo sapiens | |
dexamethasone | DD4 wild-type and mutants | Homo sapiens | |
Hexestrol | DD4 wild-type and mutants | Homo sapiens | |
indomethacin | DD4 wild-type and mutants | Homo sapiens | |
medroxyprogesterone | DD4 wild-type and mutants | Homo sapiens | |
additional information | DD4 is inhibited by steroidal anti-inflammatory drugs | Homo sapiens | |
Phenolphthalein | DD4 wild-type and mutants | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
DD4 wild-type and mutant enzymes, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | involved in the metabolism of steroid hormones and polycyclic aromatic hydrocarbons, and also in the reduction of ketone-containing drugs | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
DD4 | - |
Purification (Comment) | Organism |
---|---|
DD4 double mutant S145C/L311V recombinant from Escherichia coli | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ | DD4 is identical with human chlordecone reductase and 3alpha-hydroxysteroid dehydrogenase type 1, EC 1.1.1.213, catalyzes the oxidoreduction of various 3alpha-hydroxysteroids including bile acids and belong to the aldo-keto reductase superfamily, in this concern termed AKR1C4 | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.73 | - |
DD4 recombinant double mutant S145C/L311V, purified, substrate (S)-indan-1-ol | Homo sapiens |
1.5 | - |
DD4 recombinant mutant S145C, purified, substrate (S)-indan-1-ol | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-indan-1-ol + NADP+ | DD4, recombinant double mutant S145C/L311V | Homo sapiens | ? | - |
? | |
(R)-tetralol + NADP+ | DD4, recombinant double mutant S145C/L311V | Homo sapiens | ? | - |
? | |
(S)-indan-1-ol + NADP+ | DD4, wild-type and recombinant double mutant S145C/L311V | Homo sapiens | ? | - |
? | |
(S)-tetralol + NADP+ | DD4, recombinant double mutant S145C/L311V | Homo sapiens | ? | - |
? | |
3alpha-hydroxy steroid + NADP+ | - |
Homo sapiens | 3-keto steroid + NADPH | - |
? | |
5beta-androstan-3alpha,17beta-diol + NADP+ | DD4 wild-type and mutants | Homo sapiens | 5beta-androstan-3,17-dione + NADPH | - |
? | |
androsterone + NADPH | DD4 wild-type and mutants | Homo sapiens | ? | - |
? | |
cholic acid + NADP+ | DD4, wild-type and recombinant double mutant S145C/L311V | Homo sapiens | ? | - |
? | |
additional information | involved in the metabolism of steroid hormones and polycyclic aromatic hydrocarbons, and also in the reduction of ketone-containing drugs | Homo sapiens | ? | - |
? | |
additional information | DD4 also catalyzes oxidoreduction of 3alpha-hydroxysteroids and bile acids and is thus thought to be identical with 3alpha-hydroxysteroid dehydrogenase EC 1.1.1.213 | Homo sapiens | additional information | - |
? | |
pyridine-3-aldehyde + NADPH | DD4 wild-type and mutants | Homo sapiens | ? | - |
? | |
trans-1,2-dihydrobenzene-1,2-diol + NAD(P)+ | - |
Homo sapiens | catechol + NAD(P)H | - |
? | |
trans-naphthalene dihydrodiol + NAD(P)+ | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36000, DD4 wild-type and mutants, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
DD | - |
Homo sapiens |
dihydrodiol dehydrogenase | - |
Homo sapiens |
More | DD4 shows only 83% sequence homology to the other human DD isoforms | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at, recombinant doule mutant | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00667 | - |
trans-benzene dihydrodiol | double mutant 145C/L311V | Homo sapiens | |
0.0117 | - |
trans-benzene dihydrodiol | - |
Homo sapiens | |
0.045 | - |
5beta-Androstan-3alpha,17beta-diol | double mutant S145C/L311V | Homo sapiens | |
0.0567 | - |
(S)-indan-1-ol | double mutant S145C/L311V | Homo sapiens | |
0.0883 | - |
cholic acid | double mutant S145C/L311V | Homo sapiens | |
0.108 | - |
5beta-Androstan-3alpha,17beta-diol | - |
Homo sapiens | |
0.123 | - |
(S)-indan-1-ol | - |
Homo sapiens | |
0.233 | - |
cholic acid | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens |