Cloned (Comment) | Organism |
---|---|
expression of His-tagged protein in Escherichia coli | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
C145S | same sensitivity to SH-reagents as wild-type | Bos taurus |
C193S | no sensitivity to SH-reagents remaining | Bos taurus |
D50N | low dehydrogenase activity | Bos taurus |
H117Q | increased reductase activity | Bos taurus |
K84N | low dehydrogenase activity, no remaining reductase activity | Bos taurus |
Y55F | very low dehydrogenase activity | Bos taurus |
Y55H | decreased dehydrogenase activity, significant increased in reductase activity | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | i.e. DTNB, inhibits wild-type enzyme and mutant C145S | Bos taurus | |
glutathione | wild-type and mutant C145S | Bos taurus | |
N-ethylmaleimide | wild-type and mutant C145S | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bos taurus | metabolic inactivation of exogeneous xenobiotics | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
isoform DD3 | - |
Purification (Comment) | Organism |
---|---|
DD3, recombinant, His-tagged protein from Escherichia coli | Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ | structure analysis reveals that the enzyme belongs to the aldo-keto reductase superfamily, like 3alpha-hydroxysteroid dehydrogenase, 17beta-hydroxysteroid dehydrogenase, 20alpha-hydroxysteroid dehydrogenase, aldose reductase, and aldehyde reductase | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-acenaphthenol + NAD(P)+ | - |
Bos taurus | ? | - |
? | |
camphorquinone + NAD(P)H | - |
Bos taurus | (1R)-1,7,7-trimethylbicyclo[2.2.1]heptane-2,3-diol + NADP+ | - |
? | |
additional information | metabolic inactivation of exogeneous xenobiotics | Bos taurus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DD | - |
Bos taurus |
dihydrodiol dehydrogenase | - |
Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
mutant H117Q of DD3 | Bos taurus |
9.7 | 10.5 | mutant K84N of DD3 | Bos taurus |
10 | - |
mutant D50N of DD3 | Bos taurus |
10.5 | - |
DD3, wild-type | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Bos taurus | |
NADPH | - |
Bos taurus |