Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.20 extracted from

  • Asada, Y.; Aoki, S.; Ishikura, S.; Usami, N.; Hara, A.
    Roles of His-79 and Tyr-180 of D-xylose/dihydrodiol dehydrogenase in catalytic function (2000), Biochem. Biophys. Res. Commun., 278, 333-337.
    View publication on PubMed
Search for more literature for 1.3.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutants, H79Q and Y180F, in Escherichia coli Macaca fuscata

Protein Variants

Protein Variants Comment Organism
H79Q site-directed mutagenesis, moderate activity with 190fold increase in Km-value, inhibition by MgCl2 and potassium phosphate Macaca fuscata
additional information sequence analysis reveals high sequence homology between dimeric mammalian dihydrodiol dehydrogenases, but no homology with monomeric or cis-dihydrodiol-specific enzymes Macaca fuscata
Y180F site-directed mutagenesis, very low activity Macaca fuscata

Inhibitors

Inhibitors Comment Organism Structure
MgCl2 inhibition of mutant enzymes H79Q and Y180F Macaca fuscata
phosphate buffer inhibitionn of mutant enzymes H79Q and Y180F Macaca fuscata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 Camphorquinone NADPH Macaca fuscata
0.51
-
 Camphorquinone mutant H79Q, NADPH Macaca fuscata
0.55
-
 NADP+ phosphate buffer 0.1 M Macaca fuscata
0.56
-
 NADPH
-
 Macaca fuscata
2.1
-
 NADP+ Tris buffer 25 mM Macaca fuscata
4.4
-
 D-xylose NADP+, 25 mM Tris buffer 25 mM Macaca fuscata
6.3
-
 D-xylose NADP+, 0.1 M phosphate buffer Macaca fuscata
14
-
 NADP+ mutant H79Q, phosphate buffer 0.1 M Macaca fuscata
28
-
 NADPH mutant H79Q Macaca fuscata
67
-
 D-xylose mutant H79Q, NADP+, 0.1 M phosphate buffer Macaca fuscata
213
-
 D-xylose mutant H79Q, NADP+, Tris buffer 25 mM Macaca fuscata
402
-
 NADP+ mutant H70Q, Tris buffer 25 mM Macaca fuscata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 2 * 36000, SDS-PAGE Macaca fuscata
70000
-
 gel filtration Macaca fuscata

Organism

Organism UniProt Comment Textmining
Macaca fuscata
-
 japanese monkey
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant protein recombinant from Escherichia coli Macaca fuscata

Reaction

Reaction Comment Organism Reaction ID
trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ identical with D-xylose dehydrogenase EC 1.1.1.179 Macaca fuscata
a

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Macaca fuscata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
camphorquinone + NAD(P)H
-
 Macaca fuscata (1R)-1,7,7-trimethylbicyclo[2.2.1]heptane-2,3-diol + NADP+
-
 ?
D-xylose + NADP+
-
 Macaca fuscata D-xylono-1,5-lactone + NADPH + H+
-
 ?
additional information monomeric forms are identical with 3alpha-hydroxysteroid dehydrogenase EC 1.1.1.50 and provide a broad substrate specificity, while dimeric forms are identical with D-xylose dehydrogenase EC 1.1.1.179 and are active with trans-dihydrodiols and dicarbonyl compounds Macaca fuscata additional information
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 36000, SDS-PAGE Macaca fuscata

Synonyms

Synonyms Comment Organism
DD
-
 Macaca fuscata
dihydrodiol dehydrogenase
-
 Macaca fuscata
More sequence analysis revealed high sequence homology between dimeric mammalian dihydrodiol dehydrogenases, but no homology with monomeric or cis-dihydrodiol-specific enzymes Macaca fuscata

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Macaca fuscata
NADPH
-
 Macaca fuscata