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Literature summary for 1.3.1.2 extracted from
- Purification and characterization of dihydropyrimidine dehydrogenase enzyme from sheep liver and determination of the effects of some anaesthetic and antidepressant drugs on the enzyme activity (2016), J. Enzyme Inhib. Med. Chem., 31, 1335-1341.
General Stability
| General Stability | Organism |
|---|---|
| maximum enzyme activity is obtained in 0.28 M phosphate buffer (pH 8.0) | Ovis aries |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| escitalopram | - |
Ovis aries |  |
| fluoxetine | - |
Ovis aries | |
| haloperidol | - |
Ovis aries | |
| lidocaine | - |
Ovis aries | |
| mirtazapine | - |
Ovis aries | |
| propofol | - |
Ovis aries | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01746 | - |
Uracil | at pH 7.4 and 25°C | Ovis aries | |
| 0.02297 | - |
NADPH | at pH 7.4 and 25°C | Ovis aries | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 111000 | - |
gel filtration | Ovis aries |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil + NADPH + H+ | Ovis aries | - |
5,6-dihydrouracil + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ovis aries | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE-cellulose column chromatography, and 2',5'-ADP Sepharose-4B column chromatography | Ovis aries |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00173 | - |
crude enzyme, at pH 7.4 and 25°C | Ovis aries |
| 0.705 | - |
after 407.5fold purification, at pH 7.4 and 25°C | Ovis aries |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil + NADPH + H+ | - |
Ovis aries | 5,6-dihydrouracil + NADP+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DPD | - |
Ovis aries |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Ovis aries |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Ovis aries |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
the enzyme remains stable after 12 h at pH 6.0 | Ovis aries |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Ovis aries | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00021 | - |
at pH 7.4 and 25°C | Ovis aries | propofol | |
| 0.01324 | - |
at pH 7.4 and 25°C | Ovis aries | fluoxetine | |
| 0.01507 | - |
at pH 7.4 and 25°C | Ovis aries | lidocaine | |
| 0.08665 | - |
at pH 7.4 and 25°C | Ovis aries | mirtazapine | |
| 0.09903 | - |
at pH 7.4 and 25°C | Ovis aries | haloperidol | |
| 1.736 | - |
at pH 7.4 and 25°C | Ovis aries | escitalopram | |
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