Application | Comment | Organism |
---|---|---|
medicine | target for inhibitor design to enhance the cytotoxical effect of 5-fluorouracil in tumor cells by inhibiting the DPD activity with 5-fluorouracil as substrate | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,6-dihydrouracil | competitive | Sus scrofa | |
ATP-ribose | competitive | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Sus scrofa | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5,6-dihydrouracil + NADP+ = uracil + NADPH + H+ | mechanism, rate-determining half-reaction for reduction of flavin by NADPH has minor rate limitation, while the following protonation of flavin at N-1 is slow | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.417 | 0.5 | purified enzyme | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thymine + NADPH | - |
Sus scrofa | dihydrothymine + NADP+ | - |
? | |
uracil + NADPH | - |
Sus scrofa | 5,6-dihydrouracil + NADP+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
investigation of pH-dependency of the reaction and inhibition by 2,6-dihydrouracil and ATP-ribose, kinetics | Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 8.5 | - |
Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | - |
Sus scrofa | |
NADPH | strictly dependent on | Sus scrofa |