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Literature summary for 1.3.1.2 extracted from

  • Rosenbaum, K.; Jahnke, K.; Curti, B.; Hagen, W.R.; Schnackerz, K.D.; Vanoni, M.A.
    Porcine Recombinant Dihydropyrimidine Dehydrogenase: Comparison of the spectroscopic and catalytic properties of the wild-type and C671A mutant enzymes (1998), Biochemistry, 37, 17598-17609.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overproduction of wild-type and mutant enzyme in Escherichia coli Sus scrofa

Protein Variants

Protein Variants Comment Organism
C671A 1% activity compared to that of the wild-type enzyme Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
NADP+
-
Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 16 iron atoms per enzyme, wild-type and mutant C671A, [4Fe-4S] clusters Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
111000
-
2 * 111000, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thymine + NADPH Sus scrofa
-
dihydrothymine + NADP+
-
?
uracil + NADPH Sus scrofa rate limiting enzyme in pyrimidine degradation 5,6-dihydrouracil + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzyme Sus scrofa

Reaction

Reaction Comment Organism Reaction ID
5,6-dihydrouracil + NADP+ = uracil + NADPH + H+ comparison of substrate and cofactor binding to wild-type and mutant enzyme Sus scrofa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0043
-
mutant C671A Sus scrofa
0.43
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,6-dihydrouracil + NADP+ C671A mutant only forms complex with dihydrouracil nearly without oxidizing it Sus scrofa uracil + NADPH
-
r
thymine + NADPH
-
Sus scrofa dihydrothymine + NADP+
-
?
uracil + NADPH
-
Sus scrofa 5,6-dihydrouracil + NADP+
-
r
uracil + NADPH rate limiting enzyme in pyrimidine degradation Sus scrofa 5,6-dihydrouracil + NADP+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 111000, SDS-PAGE Sus scrofa

Cofactor

Cofactor Comment Organism Structure
flavin contains 2 mol FMN and 2 mol FAD per mol of enzyme, tightly associated Sus scrofa
NADPH
-
Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.001
-
NADP+
-
Sus scrofa