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Literature summary for 1.3.1.19 extracted from

  • Fong, K.P.Y.; Tan, H.M.
    Characterization of a novel cis-benzene dihydrodiol dehydrogenase from Pseudomonas putida ML2 (1999), FEBS Lett., 451, 5-9.
    View publication on PubMed

General Stability

General Stability Organism
unstable Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
cis-1,2-dihydrobenzene-1,2-diol cosubstrate NAD+ Pseudomonas putida
0.22
-
NAD+
-
Pseudomonas putida
46
-
glycerol cosubstrate NAD+ Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal ions requirement Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
4 * 39000, SDS-PAGE Pseudomonas putida
166000
-
PAGE Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cis-1,2-dihydrobenzene-1,2-diol + NAD+ Pseudomonas putida
-
catechol + NADH
-
?
cis-1,2-dihydrobenzene-1,2-diol + NAD+ Pseudomonas putida ML2
-
catechol + NADH
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida ML2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
dye-affinity chromatography and NAD+ agarose chromatography Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
68
-
glycerol, NAD+ Pseudomonas putida
153
-
cis-1,2-dihydrobenzene-1,2-diol, NAD+ Pseudomonas putida
313
-
cis-1,2-cyclohexanediol, NAD+ Pseudomonas putida
513
-
1,2-propanediol, NAD+ Pseudomonas putida

Storage Stability

Storage Stability Organism
less stable at 4°C than at -20°C, loss of activity at 4°C gradually over a few days Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-propanediol + NAD+
-
Pseudomonas putida propen-1,2-diol + NADH
-
?
1,2-propanediol + NAD+
-
Pseudomonas putida ML2 propen-1,2-diol + NADH
-
?
cis-1,2-cyclohexanediol + NAD+
-
Pseudomonas putida cyclohex-1-ene-1,2-diol + NADH
-
?
cis-1,2-cyclohexanediol + NAD+
-
Pseudomonas putida ML2 cyclohex-1-ene-1,2-diol + NADH
-
?
cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
Pseudomonas putida catechol + NADH
-
?
cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
Pseudomonas putida ML2 catechol + NADH
-
?
glycerol + NAD+
-
Pseudomonas putida ?
-
?
glycerol + NAD+
-
Pseudomonas putida ML2 ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 39000, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
cis-benzene dihydrodiol dehydrogenase bedD enzyme Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
enzyme assay Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
with NAD+ as cofactor, 100 mM potassium carbonate buffer Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas putida