Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain MC1061 | Lactococcus lactis |
two different genes | Lactococcus lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34120 | - |
deduced from sequence of cDNA | Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | - |
- |
- |
Lactococcus lactis | A2RJT9 | subspecies Lactococcus lactis cremoris, strain MG1363 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.015 | - |
native wild type enzyme, using NAD+ as acceptor, at 30°C | Lactococcus lactis |
0.05 | - |
recombinant wild type enzyme, using NAD+ as acceptor, at 37°C | Lactococcus lactis |
0.06 | - |
native wild type enzyme, using 2,6-dichlorophenolindophenol as acceptor, at 30°C | Lactococcus lactis |
5.9 | - |
recombinant wild type enzyme, using 2,6-dichlorophenolindophenol as acceptor, at 37°C | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + 2,6-dichlorophenolindophenol | - |
Lactococcus lactis | orotate + reduced 2,6-dichlorophenolindophenol | - |
? | |
(S)-dihydroorotate + NAD+ | - |
Lactococcus lactis | orotate + NADH + H+ | - |
? | |
dihydroorotate + acceptor | acceptor: NAD+ for pyrDa gene product, fumarate for pyrDb gene product | Lactococcus lactis | orotate + reduced acceptor | - |
? | |
additional information | O2, fumarate, and NADP+ do not serve as electron acceptors | Lactococcus lactis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHOdehase A | - |
Lactococcus lactis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Lactococcus lactis |