Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanothrix harundinacea |
Protein Variants | Comment | Organism |
---|---|---|
Q227E | mutation introduces arogenate dehydrogenase activity | Methanothrix harundinacea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
prephenate | wild-type, pH 7.6, temperature not specified in the publication | Methanothrix harundinacea | |
2.67 | - |
prephenate | mutant Q227E, pH 7.6, temperature not specified in the publication | Methanothrix harundinacea | |
3.29 | - |
L-arogenate | mutant Q227E, pH 7.6, temperature not specified in the publication | Methanothrix harundinacea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothrix harundinacea | A0A101IGG2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arogenate + NADP+ | no substrate of wild-type, reaction of EC 1.3.1.78 | Methanothrix harundinacea | L-tyrosine + NADPH + H+ + CO2 | - |
? | |
prephenate + NADP+ | - |
Methanothrix harundinacea | 4-hydroxyphenylpyruvate + NADPH + H+ + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tyrA | - |
Methanothrix harundinacea |
XD72_2094 | - |
Methanothrix harundinacea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
prephenate | mutant Q227E, pH 7.6, temperature not specified in the publication | Methanothrix harundinacea | |
0.7 | - |
L-arogenate | mutant Q227E, pH 7.6, temperature not specified in the publication | Methanothrix harundinacea | |
2.44 | - |
prephenate | wild-type, pH 7.6, temperature not specified in the publication | Methanothrix harundinacea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | enzyme strongly prefers NADP+ over NAD+ | Methanothrix harundinacea | |
NADP+ | enzyme strongly prefers NADP+ over NAD+ | Methanothrix harundinacea |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
prephenate | mutant Q227E, pH 7.6, temperature not specified in the publication | Methanothrix harundinacea | |
0.21 | - |
L-arogenate | mutant Q227E, pH 7.6, temperature not specified in the publication | Methanothrix harundinacea | |
6.44 | - |
prephenate | wild-type, pH 7.6, temperature not specified in the publication | Methanothrix harundinacea |