Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Nanoarchaeum equitans |
expressed in Escherichia coli BL21(DE3) cells | Ignicoccus hospitalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by L-Tyr | Ignicoccus hospitalis | |
additional information | not inhibited by L-Tyr | Nanoarchaeum equitans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.062 | - |
NADP+ | at pH 8.0 and 80°C | Ignicoccus hospitalis | |
0.319 | - |
prephenate | with NAD+ as cosubstrate, at pH 8.0 and 80°C | Ignicoccus hospitalis | |
0.36 | - |
NADP+ | at pH 8.0 and 80°C | Nanoarchaeum equitans | |
0.443 | - |
prephenate | with NADP+ as cosubstrate, at pH 8.0 and 80°C | Ignicoccus hospitalis | |
0.561 | - |
NAD+ | at pH 8.0 and 80°C | Ignicoccus hospitalis | |
0.69 | - |
prephenate | with NAD+ as cosubstrate, at pH 8.0 and 80°C | Nanoarchaeum equitans | |
0.73 | - |
prephenate | with NADP+ as cosubstrate, at pH 8.0 and 80°C | Nanoarchaeum equitans | |
3.3 | - |
NAD+ | at pH 8.0 and 80°C | Nanoarchaeum equitans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71000 | - |
analytical ultracentrifugation | Nanoarchaeum equitans |
85000 | - |
analytical ultracentrifugation | Ignicoccus hospitalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
prephenate + NAD+ | Nanoarchaeum equitans | - |
4-hydroxyphenylpyruvate + CO2 + NADH | - |
? | |
prephenate + NAD+ | Ignicoccus hospitalis | - |
4-hydroxyphenylpyruvate + CO2 + NADH | - |
? | |
prephenate + NADP+ | Nanoarchaeum equitans | the enzyme prefers NADP+ over NAD+ | 4-hydroxyphenylpyruvate + CO2 + NADPH | - |
? | |
prephenate + NADP+ | Ignicoccus hospitalis | the enzyme prefers NADP+ over NAD+ | 4-hydroxyphenylpyruvate + CO2 + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ignicoccus hospitalis | A8AAX2 | - |
- |
Nanoarchaeum equitans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, and Superdex 200 gel filtration | Nanoarchaeum equitans |
Ni-NTA column chromatography, and Superdex 200 gel filtration | Ignicoccus hospitalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
prephenate + NAD+ | - |
Nanoarchaeum equitans | 4-hydroxyphenylpyruvate + CO2 + NADH | - |
? | |
prephenate + NAD+ | - |
Ignicoccus hospitalis | 4-hydroxyphenylpyruvate + CO2 + NADH | - |
? | |
prephenate + NADP+ | the enzyme prefers NADP+ over NAD+ | Nanoarchaeum equitans | 4-hydroxyphenylpyruvate + CO2 + NADPH | - |
? | |
prephenate + NADP+ | the enzyme prefers NADP+ over NAD+ | Ignicoccus hospitalis | 4-hydroxyphenylpyruvate + CO2 + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Nanoarchaeum equitans |
homodimer | - |
Ignicoccus hospitalis |
Synonyms | Comment | Organism |
---|---|---|
CM-PD | bifunctional enzyme with chorismate mutase and prephenate dehydrogenase activities | Ignicoccus hospitalis |
PD-CM-PDT | trifunctional enzyme with chorismate mutase, prephenate dehydrogenase and prephenate dehydratase activities | Nanoarchaeum equitans |
tyrA | - |
Nanoarchaeum equitans |
tyrA | - |
Ignicoccus hospitalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Nanoarchaeum equitans |
90 | - |
- |
Ignicoccus hospitalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 95 | the enzyme retains full dehydrogenase activity after 50 h incubation at 80°C and greater than 50% of specific activity after 50 h at 95°C | Nanoarchaeum equitans |
80 | 95 | the enzyme retains full dehydrogenase activity after 50 h incubation at 80°C and greater than 50% of specific activity after 50 h at 95°C | Ignicoccus hospitalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Nanoarchaeum equitans | |
NAD+ | - |
Ignicoccus hospitalis | |
NADP+ | the enzyme prefers NADP+ over NAD+ | Nanoarchaeum equitans | |
NADP+ | the enzyme prefers NADP+ over NAD+ | Ignicoccus hospitalis |